Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지
DOI QR코드

DOI QR Code

Analysis of Plasminogen Activators Activity and Three Dimensional (3D) Culture of Endometrial Cells in Pigs

돼지 자궁내막 세포의 3차원 배양과 Plasminogen Activator 활성화 분석

  • Cha, Hye-Jin (Collage of Animal Life Science, Kangwon National University) ;
  • Lee, Sang-Hee (Collage of Animal Life Science, Kangwon National University) ;
  • Cheong, Hee-Tae (Collage of Veterinary Medicine, Kangwon National University) ;
  • Yang, Boo-Keun (Collage of Animal Life Science, Kangwon National University) ;
  • Park, Choon-Keun (Collage of Animal Life Science, Kangwon National University)
  • 차혜진 (강원대학교 동물생명과학대학) ;
  • 이상희 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의과대학) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 박춘근 (강원대학교 동물생명과학대학)
  • Received : 2013.08.19
  • Accepted : 2013.09.03
  • Published : 2013.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to establish a three dimensional (3D) culture system of endometrial cells and to examine the plasminogen activators (PAs) activity in porcine uterine. The 3D culture system in porcine endometrial cells was composed to mixture 3D gel, stromal cells and epithelial cells. The 3D culture system was used to identify normal structure search as uterine tissue and PAs expression in this study. In results, porcine endometrium epithelial cells forming a top monolayer and endometrium stromal cells developed as fibroblast-like within 3D matrix scaffold. Expression of urokinase-type PA (uPA) and tissue-type PA (tPA) were observed during the 3D culture using immunofluorescence. PA activity in 3D-cultured endometrial cells was no significant difference between the tissue type, but 2D culture system were significantly lower than in 3D-cultured endometrial cells (P<0.05). Therefore, basic system and functional aspect of 3D culture could be established with similar system of endometrium tissue. We suggest that this study was assumed applicable as baseline data to investigate mechanism between porcine uterus cells in vitro.

Keywords

References

  1. Ashkenas J, Muschler J and Bissell MJ. 1996. The extracellular matrix in epithelial biology: shared molecules and common themes in distant phyla. Dev. Biol. 180: 433-444. https://doi.org/10.1006/dbio.1996.0317
  2. Birgitta O, Eija K, Michael SP, Stefano JM, Karin A. Vijay K, Yuji G, Michael M, Jeltsch MS, Kari A and Ulf E. 1998. Vascular endothelial growth factor B (VEGF-B) binds to VEGF receptor-1 and regulates plasminogen activator activity in endothelial cells Proc. Natl. Acad. Sci. USA. 95: 11709-11714. https://doi.org/10.1073/pnas.95.20.11709
  3. Boudreau NJ. 2003. Organized living: from cell surfaces to basement membranes. Sci. STKE. 196: 34.
  4. Casslen B, Nordengren J, Gustavsson B, Nilbert M and Lund LR. 1995. Progesterone stimulates degradation of urokinase plasminogen activator (u-PA) in endometrial stromal cells by increasing its inhibitor (PAI1) and surface expression of the u-PA receptor. J. Clin. Endocrinol. Metab. 80: 2776-2784.
  5. Choi BK. 2011. Thermal gelling polyalanine-poloxamine-polyalanine aqueous solution for chondrocytes 3D culture: Initial concentration effect. Soft Matter 7: 456-462. https://doi.org/10.1039/c0sm00611d
  6. Choi CW, Cho WM, Yeon SH, Hwang HS and Song MG. 2012. Comparison between single and co-culture of adipocyte and muscle cell lines in cell morphology and cytosolic substances. Annals of Animal Resource Sciences 54: 103-109.
  7. Gething MJ, Adler B, Boose JA, Gerard RD, Madison EL, McGookey D, Meidell RS, Roman LM and Sambrook J. 1988. Variants of human tissue-type plasminogen activator that lack specific structural domains of the heavy chain. The EMBO. 7: 2731-2740.
  8. Hai W, Federica P, Sandra A, Sebastia ME, Isabel H, Juan MMM, Sirisha M, Silvina B, Sergio O, and JoseAH. 2012. A novel model of human implantation: 3D endometrium-like culture system to study attachment of human trophoblast (Jar) cell spheroids. Mol. Hum. Reprod. 18: 33-43. https://doi.org/10.1093/molehr/gar064
  9. Hart DA and Rehemtulla A. 1988. Plasminogen activators and their inhibitors: regulators of extracellular proteolysis and cell function. Comparative Biochem. and Physiol. B 90: 691-708. https://doi.org/10.1016/0305-0491(88)90323-9
  10. Hay ED. 2005. The mesenchymal cell, its role in the embryo, and the remarkable signaling mechanisms that create it. Dev. Dyn. 233: 706-720. https://doi.org/10.1002/dvdy.20345
  11. Hearn JP. 1986. The embryo-maternal dialogue during early pregnancy in primates. J. Reprod. Fertil. 76: 809-19. https://doi.org/10.1530/jrf.0.0760809
  12. Hilde V, Paul S and Henri L. 2000. Immunohistochemical detection of the estrogen receptora and progesterone receptor in the canine pregnant uterus and placental labyrinth. The Anatomical Record. 260: 42-50. https://doi.org/10.1002/1097-0185(20000901)260:1<42::AID-AR50>3.0.CO;2-8
  13. Inaba T, Wiest WG, Strickler RC and Mori J. 1988. Augmentation of the response of mouse uterine epithelial cells to estradiol by uterine stroma. Endocrinology 123: 1253-1258. https://doi.org/10.1210/endo-123-3-1253
  14. Kalluri R. 2003. Basement membranes: structure, assembly and role in tumour angiogenesis. Nat. Rev. Cancer 3: 422-433. https://doi.org/10.1038/nrc1094
  15. Kim KH, Lee YS, Gu HN, Yang BK, Cheong HT and Park CK. 2011. Changes in plasminogen activity in uterus tissue during the estrous cycle in the pigs. Reprod. Dev. Biol. 35: 463-468.
  16. Kwon HC, Yang HW, Kim HK, Kim SK and Cho DJ. 2001. Establishment of three dimensional in vitro culture system with human endometrial cells: induction of molecular biologic differentiation & characterization by sex steroid hormone. Korean J. Obstet. Gynecol. 44: 65-73.
  17. Liu Y. 1999. Role of plasminogen activators and inhibitors in reproduction. Chinese Science Bulletin 44: 673-685. https://doi.org/10.1007/BF02909700
  18. Martin O and Arias F. 1982. Plasminogen activator production by trophoblast cells in vitro. Effect of steroid hormones and protein synthesis inhibitors. J. Obstetrics and Gynecology 142: 402-409.
  19. Park DW, Yang HW, Kwon HC, Jang KH, Kim SG, Cho DJ and Oh GS. 1999. 3-Dimensional culture system of endometrial cells for studying the human implantation mechanism. Korean J. Reprod. Med. 26: 1-4.
  20. Sandberg T, Casslen B, Gustavsson B and Benraad TJ. 1998. Human endothelial cell migration is stimulated by urokinase plasminogen activator: plasminogen activator inhibitor 1 complex released from endometrial stromal cells stimulated with transforming growth factor ${\beta}1$; possible mechanism for paracrine stimulation of endometrial angiogenesis. Biol. Reprod. 59: 759-767. https://doi.org/10.1095/biolreprod59.4.759
  21. Jean DV, Daniel B and Dominique B. 1985. A cellular binding site for the Mr 55,000 form of the human plasminogen activator, urokinase. J. Cell Biol. 100: 86-92. https://doi.org/10.1083/jcb.100.1.86
  22. Victoria S, Svetlana M, Eleonore K, Theese JR, Paul E and Vsevolod AT. 1999. Urokinase plasminogen activator induces human smooth muscle cell migration and proliferation via distinct receptor-dependent and proteolysis dependent mechanisms. Mol. Cell Biochem. 195: 199-206. https://doi.org/10.1023/A:1006936623106