한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제28권3호
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  • Pages.257-263
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  • 2013
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
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돼지의 배란 전 자궁내막 상피세포 내 Plasminogen Activators의 발현

Expression of Plasminogen Activators in Uterine Epithelial Cells of Pre-ovulatory Phase in Pigs

  • 황보용 (강원대학교 동물생명과학대학) ;
  • 이상희 (강원대학교 동물생명과학대학) ;
  • 차혜진 (강원대학교 동물생명과학대학) ;
  • 송은지 (강원대학교 동물생명과학대학) ;
  • 이승태 (강원대학교 동물생명과학대학) ;
  • 이은송 (강원대학교 수의과대학) ;
  • 정희태 (강원대학교 수의과대학) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 박춘근 (강원대학교 동물생명과학대학)
  • HwangBo, Yong (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Sang-Hee (College of Animal Life Sciences, Kangwon National University) ;
  • Cha, Hye-Jin (College of Animal Life Sciences, Kangwon National University) ;
  • Song, Eun-Ji (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Seung-Tae (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Eun-Song (College of Veterinary Medicine, Kangwon National University) ;
  • Cheong, Hee-Tae (College of Veterinary Medicine, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
  • 투고 : 2013.08.16
  • 심사 : 2013.09.03
  • 발행 : 2013.09.30
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초록

The endometrium undergoes a cyclic growth and tissue remodeling as changes of epithelial cells, and plasminogen activators (PAs) are related to endometrium tissue remodeling. This study was to evulate expression of urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) in porcine uterine epithelial cells. In results, the uPA and tPA were expressed in uterine tissue, epithelium and secretory glands in porcine endometrial cell. In addition, the uPA and tPA were expressed in cultured epithelial cells, and it were mainly expressed in cytoplasm. In porcine uterine tissue and epithelial cells, uPA activity was higher than activity in tPA. In PAs mRNA expression levels, uPA mRNA level was significantly higher than tPA mRNA level (P<0.05). The fluorescence intensity of uPA protein was also higher than fluorescence intensity of tPA protein, and uPA protein expression was significantly higher than in tPA protein expression (P<0.05). Therefore, we suggest that a physiological function in porcine uterine epithelial cells should be more influenced by uPA than in tPA during pre-ovulatory phase.

키워드

참고문헌

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