Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of Hh-Ag1.5 Treatment on the In Vitro Development and Apoptosis of In Vitro Fertilized Embryos in Pigs

Hh-Ag1.5 처리가 돼지 체외수정란의 발육 및 세포사멸에 미치는 영향

  • Kwon, Dae-Jin (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Yeo, Jae-Hun (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Noh, Won-Gun (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Kwak, Tae-Uk (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Oh, Keon Bong (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Ock, SunA (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Im, Seok Ki (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Park, Jin-Ki (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Hwang, Seongsoo (Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • 권대진 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 여재훈 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 노원근 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 곽태욱 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 오건봉 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 옥선아 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 임석기 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 박진기 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 황성수 (농촌진흥청 국립축산과학원 동물바이오공학과)
  • Received : 2013.08.22
  • Accepted : 2013.09.10
  • Published : 2013.09.30
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Abstract

The present study was performed to investigate the effect of Hh-Ag1.5, a small-molecule chemical agonist of SMOothened receptor, on the in vitro maturation and development of in vitro fertilized (IVF) embryos in pigs. Oocytes or fertilized embryos were cultured in a maturation or embryo culture medium supplemented with 0 (control), 25, 50 or 100 nM of Hh-Ag1.5, respectively. Although the maturation rate were not different among treatment groups, the blastocyst formation rate in the group treated with 25 nM Hh-Ag1.5 was significantly increased compared to other groups (P<0.05). While the highest dose of Hh-Ag1.5 (100 nM) did negatively affect to the embryo development and cell number in blastocysts compared to other groups (P<0.05), the apoptotic cell index in blastocysts was significantly lower in 25 and 50 nM groups than in control and 100 nM groups (P<0.05). The mRNA expression of the proapoptotic gene Bax and the ratio of Bax/Bcl-XL decreased in among treatment groups compared to control (P<0.05). The embryo quality related genes, Tert and Zfp42, were significantly decreased in 50 and 100 nM groups compared with control and 25 nM groups (P<0.05). In conclusion, the addition of 25 nM Hh-Ag1.5 to in vitro maturation and culture medium can enhance the developmental potential as well as quality of IVF embryos in pig.

Keywords

References

  1. Abeydeera LR, Wang WH, Cantley TC, Rieke A, Prather RS and Day BN. 1998. Presence of epidermal growth factor during in vitro maturation of pig oocytes and embryo culture can modulate blastocyst development after in vitro fertilization. Mol. Reprod. Dev. 51: 395-401. https://doi.org/10.1002/(SICI)1098-2795(199812)51:4<395::AID-MRD6>3.0.CO;2-Y
  2. Bobe J, Montfort J, Nguyen T and Fostier A. 2006. Identification of new participants in the rainbow trout (Oncorhynchus mykiss) oocyte maturation and ovulation processes using cDNA microarrays. Reprod. Biol. Endocrinol. 4: 39-54. https://doi.org/10.1186/1477-7827-4-39
  3. Carrell DT, Liu L, Huang I and Peterson CM. 2005. Comparison of maturation, meiotic competence, and chromosome aneuploidy of oocytes derived from two protocols for in vitro culture of mouse secondary follicles. J. Assist. Reprod. Gen. 22: 347-354. https://doi.org/10.1007/s10815-005-6793-2
  4. Dahse R, Fiedler W and Ernst G. 1997. Telomeres and telomerase: biological and clinical importance. Clin. Chem. 43: 708-714.
  5. Frank-Kamenetsky M, Zhang XM, Bottega S, Guicherit O, Wichterle H, Dudek H, Bumcrot D, Wang FY, Jones S, Shulok J, Rubin LL and Porter JA. 2002. Small-molecule modulators of Hedgehog signaling: identification and characterization of SMOothened agonists and antagonists. J. Biol. 1: 10-28. https://doi.org/10.1186/1475-4924-1-10
  6. Gandolfi TA and Gandolfi F. 2001. The maternal legacy to the embryo: cytoplasmic components and their effects on early development. Theriogenology 55: 1255-1276. https://doi.org/10.1016/S0093-691X(01)00481-2
  7. Kim S, Lee SH, Kim JH, Jeong YW, Hashem MA, Koo OJ, Park SM, Lee EG, Hossein MS, Kang SK, Lee BC and Hwang WS. 2006. Anti-apoptotic effect of insulin-like growth factor (IGF)-I and its receptor in porcine preimplantation embryos derived from in vitro fertilization and somatic cell nuclear transfer. Mol. Reprod. Dev. 73: 1523-1530. https://doi.org/10.1002/mrd.20531
  8. Lee K, Jeong J, Kwak I, Yu CT, Lanske B, Soegiarto DW, Toftgard R, Tsai MJ, Tsai S, Lydon JP and DeMayo FJ. 2006. Indian hedgehog is a major mediator of progesterone signaling in the mouse uterus. Nat. Genet. 38: 1204-1209. https://doi.org/10.1038/ng1874
  9. Lonergan P, Fair T, Corcoran D and Evans AC. 2006. Effect of culture environment on gene expression and developmental characteristics in IVF-derived embryos. Theriogenology 65: 137-152. https://doi.org/10.1016/j.theriogenology.2005.09.028
  10. Malcuit C, Trask MC, Santiago L, Beaudoin E, Tremblay KD and Mager J. 2009. Identification of novel oocyte and granulosa cell markers. Gene Expr. Patterns. 9: 404-410. https://doi.org/10.1016/j.gep.2009.06.004
  11. Nguyen NT, Lin DPC, Yen SY, Tseng JK, Chuang JF, Chen BY, Lin TA, Chang HH and Ju JC. 2009. Sonic Hedgehog promotes porcine oocyte maturation and early embryo development. Reprod. Fertil. Dev. 21: 805-815. https://doi.org/10.1071/RD08277
  12. Nguyen NT, Lin DP, Siriboon C, Lo NW and Ju JC. 2010. Sonic Hedgehog improves in vitro development of porcine parthenotes and handmade cloned embryos. Theriogenology 74: 1149-1160. https://doi.org/10.1016/j.theriogenology.2010.05.016
  13. Nguyen NT, Lo NW, Chuang SP, Jian YL and Ju JC. 2011. Sonic Hedgehog supplementation of oocyte and embryo culture media enhances development of IVF porcine embryos. Reproduction 142: 87-97. https://doi.org/10.1530/REP-11-0049
  14. Prather RS, Hawley RJ, Carter DB, Lai L and Greenstein JL. 2003. Transgenic swine for biomedicine and agriculture. Theriogenology 59: 115-123. https://doi.org/10.1016/S0093-691X(02)01263-3
  15. Ren Y, Cowan RG, Harman RM and Quirk SM. 2009. Dominant activation of the Hedgehog signaling pathway in the ovary alters theca development and prevents ovulation. Mol. Endocrinol. 23: 711-723. https://doi.org/10.1210/me.2008-0391
  16. Rogers MB, Hosler BA and Gudas LJ. 1991. Specific expression of a retinoic acid-regulated, zinc-finger gene, Rex-1, in preimplantation embryos, trophoblast and spermatocytes. Development 113: 815-824.
  17. Sirard MA, Richard F, Blondin P and Robert C. 2006. Contribution of the oocyte to embryo quality. Theriogenology 65: 126-136. https://doi.org/10.1016/j.theriogenology.2005.09.020
  18. Spicer LJ, Sudo S, Aad PY, Wang LS, Chun SY, Ben-Shlomo I, Klein C and Hsueh AJ. 2009. The Hedgehog-patched signaling pathway and function in the mammalian ovary: a novel role for Hedgehog proteins in stimulating proliferation and steroidogenesis of theca cells. Reproduction 138: 329-339. https://doi.org/10.1530/REP-08-0317
  19. Vandaele L, Mateusen B, Maes DG, de Kruif A and Van Soom A. 2007. Temporal detection of caspase-3 and -7 in bovine in vitro produced embryos of different developmental capacity. Reproduction 133: 709-718. https://doi.org/10.1530/REP-06-0109
  20. Xu J and Yang X. 2001. Telomerase activity in early bovine embryos derived from parthenogenetic activation and nuclear transfer. Biol. Reprod. 64: 770-774. https://doi.org/10.1095/biolreprod64.3.770
  21. Xu XF, Guo CY, Liu J, Yang WJ, Xia YJ, Xu L, Yu YC and Wang XP. 2009. Gli1 maintains cell survival by up-regulating IGFBP6 and Bcl-2 through promoter regions in parallel manner in pancreatic cancer cells. J. Carcinog. 8: 13-37. https://doi.org/10.4103/1477-3163.55429
  22. Zheng P and Dean J. 2007. Oocyte-specific genes affect folliculogenesis, fertilization, and early development. Semin. Reprod. Med. 25: 243-251. https://doi.org/10.1055/s-2007-980218