Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Combined Genotoxic Effects of Aflatoxin B1, Ochratoxin A and Zearalenone in Rat Bone Marrow and Blood Leukocytes

  • Tigran, Harutyunyan (Yerevan State University, Faculty of Biology, Department of Genetics and Cytology) ;
  • Anna, Karapetyan (Yerevan State University, Faculty of Biology, Department of Genetics and Zoology) ;
  • Galina, Hovhannisyan (Yerevan State University, Faculty of Biology, Department of Genetics and Cytology) ;
  • Rouben, Aroutiounian (Yerevan State University, Faculty of Biology, Department of Genetics and Cytology)
  • Received : 2013.04.13
  • Accepted : 2013.08.12
  • Published : 2013.09.30
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Abstract

Mycotoxins such as aflatoxin B1 (AFB1), ochratoxin A (OTA) and zearalenone (ZEA) are widespread contaminants of food and feedstuffs. It is very likely, that humans and animals are always exposed to mixtures of mycotoxins rather than to individual compounds. Therefore, risk assessments should consider mixture toxicity data. In the present study the combination of AFB1, OTA and ZEA was tested for genotoxicity in rat bone marrow and blood leukocytes after 15, 30 and 60 days treatment. The level of DNA damage was determined by the comet assay. The tail intensity and Olive tail moment in leukocytes and bone marrow cells were significantly higher than in controls. At the same time, the level of DNA damage in bone marrow cells was higher than in leukocytes. The data suggests that prolonged exposure to mycotoxins combination through food consumption can induce DNA damage contributing to the harmful effects in vivo.

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References

  1. Ben Salah-Abbes J, S Abbes, Z Ouanes, MA Abdel-Wahhab, H Bacha and R Oueslati. 2009. Isothiocyanate from the Tunisian radish (Raphanus sativus) prevents genotoxicity of Zearalenone in vivo and in vitro. Mutat. Res. 677:59-65. https://doi.org/10.1016/j.mrgentox.2009.05.017
  2. Brahmi D, C Bouaziz, Y Ayed, H Ben Mansour, L Zourgui and H Bacha. 2011. Chemopreventive effect of cactus Opuntia ficusindica on oxidative stress and genotoxicity of aflatoxin B1. Nutr. Metab. 8:73. https://doi.org/10.1186/1743-7075-8-73
  3. Kamp HG, G Eisenbrand, C Janzowski, J Kiossev, JR Latendresse, J Schlatter and RJ Turesky. 2005. Ochratoxin A induces oxidative DNA damage in liver and kidney after oral dosing to rats. Mol. Nutr. Food Res. 49:1160-1167. https://doi.org/10.1002/mnfr.200500124
  4. Ouanes Z, I Ayed-Boussema, T Baati, EE Creppy and H Bacha. 2005. Zearalenone induces chromosome aberrations in mouse bone marrow: preventive effect of 17 beta-estradiol, progesterone and Vitamin E. Mutat. Res. 565:139-149. https://doi.org/10.1016/j.mrgentox.2004.10.005
  5. Pfohl-Leszkowicz A and RA Manderville. 2007. Ochratoxin A: an overview on toxicity and carcinogenicity in animals and humans. Mol. Nutr. Food Res. 51:61-99. https://doi.org/10.1002/mnfr.200600137
  6. Segvic Klaric M. 2012. Adverse effects of combined mycotoxins. Arh. Hig. Rada. Toksikol. 63:519-530.
  7. Tice RR, E Agurell, D Anderson, B Burlinson, A Hartmann, H Kobayashi, Y Miyamae, E Rojas, JC Ryu and YF Sasaki. 2000. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35:206-221. https://doi.org/10.1002/(SICI)1098-2280(2000)35:3<206::AID-EM8>3.0.CO;2-J
  8. Zeljezic D, AM Domijan and M Peraica. 2006. DNA damage by ochratoxin A in rat kidney assessed by the alkaline comet assay. Braz. J. Med. Biol. Res. 39:1563-1568. https://doi.org/10.1590/S0100-879X2006005000029