한국패류학회지 (The Korean Journal of Malacology)

한국패류학회 (The Malacological Society of Korea)
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삼배체와 이배체 굴, Crassostrea gigas의 형태 및 생리학적 특성 비교

Morphological and physiological comparison between triploid and diploid Pacific Oyster, Crassostrea gigas

  • 김수경 (국립수산과학원 서해수산연구소) ;
  • 심나영 (국립수산과학원 서해수산연구소) ;
  • 이원영 (국립수산과학원 서해수산연구소) ;
  • 최민섭 (국립수산과학원 서해수산연구소) ;
  • 최은희 (국립수산과학원 서해수산연구소) ;
  • 임현정 (국립수산과학원 서해수산연구소)
  • 투고 : 2013.08.29
  • 심사 : 2013.09.26
  • 발행 : 2013.09.30
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초록

The morphological relations and physiological characteristics of the triploid and diploid oysters (Crassostrea gigas) in Taean area, west coast of Korea, were investigated from May 2012 to April 2013. Mophometric analysis indicated that the triploid oysters have the same shell length to shell height ratio but higher shell depth to shell height ratio than diploids. Consistent with morphological characteristics, triploid oysters showed greater values of fatness, condition index and RNA/DNA ratio during the period of experiment. The DNA concentration in adductor muscle and mantle of triploid were either lower or equal to the nucleic acids of diploid. However, RNA/DNA ratio were significantly higher than diploid. It appears that RNA/DNA ratio could be a useful indicator of health condition of triploid and diploid oysters when taken in correlation with the morphological indices.

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참고문헌

  1. Akashige, S. and Fushimi, T. (1992). Growth, survival and glycogen content of triploid Pacific oyster Crassostrea gigas in the waters of Hiroshima, Japan. Nippon Suisan Gakkaishi, 58: 1063-1071. https://doi.org/10.2331/suisan.58.1063
  2. Allen Jr., S.K. (1983). Flow cytometry: Assaying experimental polyploid fish and shellfish. Aquaculture, 33:317-328. https://doi.org/10.1016/0044-8486(83)90412-X
  3. Allen Jr., S.K. and Downing, S.L. (1986). Performance of triploid pacific oysters, Crassostrea gigas (Thunberg). I. Survival, growth, glycogen content and sexual maturation in yearlings. Journal of Experimental Marine Biology and Ecology, 102: 197-208. https://doi.org/10.1016/0022-0981(86)90176-0
  4. Benfey, T.J. and Sutterlin, A.M. (1984). The haematology of triploid landlocked Atlantic salmon Salmo salar L. Journal of Fish Biology, 24: 333-338. https://doi.org/10.1111/j.1095-8649.1984.tb04804.x
  5. Evseev, G.A., Yakovlev, Y.M. and Li, X. (1996). The anatomy of the Pacific oyster, Crassostrea gigas (Thunberg) (Bivalvia: Ostreidea), Publications of the Seto Marine Biological Laboratory, 37:239-255. https://doi.org/10.5134/176265
  6. Chation, J.A. and Allen Jr, S.K. (1985). Early detection of triploidy in the larvae of Pacific oysters, Crassostrea gigas, by flow cytometry. Aquaculture, 48: 35-43. https://doi.org/10.1016/0044-8486(85)90050-X
  7. Hawkins, A.J.S., Day, A.J., Gerard, A., Naciri, Y., Ledu, C., Bayne, B.L. and Heral, M. (1994). A genetic and metabolic basis for faster growth among triploids induced by blocking meiosis I but not meiosis II in the larviparous european flat oyster, Ostrea edulis L., Journal of Experimental Marine Biology and Ecology, 184: 21-40 https://doi.org/10.1016/0022-0981(94)90164-3
  8. Hur, Y.B., Min, K.S. Kim, T.E. Lee, S.J. and Hur, S.B. (2008) Larvae growth and biochemical composition change of the Pacific oyster, Crassostrea gigas, larvae during artificial seed production. Journal of Aquaculture, 21(4): 203-212 (in Korean).
  9. Frankauser, G. (1941). Cell size, organ and body size in triploid newts (Triturus viridescens). Journal of Morphology, 68: 161-177. https://doi.org/10.1002/jmor.1050680109
  10. Fankhauser, G. (1945). The effects of changes in chromosome number on amphibian development. Quarterly Review of Biology, 20: 20-78. https://doi.org/10.1086/394703
  11. Garnier-Gere, P.H., Naciri-Graven, Y., Bougrier, Heral, M., Kotoulas, G., Haqwkins, A., and Gerard, A. (2002). Influences of triploidy, parentage and genetic diversity on growth of the Pacific oyster Crassostrea gigas reared in contrasting natural environments. Molecular Ecology, 11: 1499-1514. https://doi.org/10.1046/j.1365-294X.2002.01531.x
  12. Greenlee, A.R., Kersten, C.A. and Cloud, J.G. (1995). Effects of triploidy on rainbow trout myogenesis in vitro. Journal of Fish Biology, 46: 381-388. https://doi.org/10.1111/j.1095-8649.1995.tb05978.x
  13. Kim, S.K., Rosenthal, H., Clemmesen, C., Park, K.Y, Kim, D.H., Choi, Y.S. and Seo, H.C., (2005) Various methods to determine the gonadal development and spawning season of the purplish washington clam, Saxidomus purpuratus (Sowerby). Journal of applied lchthyology, 21: 101-106. https://doi.org/10.1111/j.1439-0426.2004.00636.x
  14. Kim, S.K., Choi, E.H., Han, H.S. and Lim, H.J. (2012). Ecophysiological characteristics changes in the Pacific oyster, Crassostrea gigas, after spawning season in off-bottom culture. Korean Journal of Malacology, 28(3): 215-223. https://doi.org/10.9710/kjm.2012.28.3.215
  15. Li, Y., Qin, J.G., Li, X. and Benkendorff, K. (2009). Monthly variation of condition index, energy reserves and antibacterial activity in Pacific oysters, Crassostrea gigas, in Stansbury (South Australia). Aquaculture, 286: 64-71. https://doi.org/10.1016/j.aquaculture.2008.09.004
  16. Lim, H.J., Lee, T.S., Cho, P.G., Back, S.H., Byun, S.G. and Choi, E.H. (2011). The production efficiency of cupped oyster, Crassostrea gigas Spat according to clutch aqnd growth comparing diploid and triploid oysters in off-bottom culture for tidal flat utilization. Korean Society of Fisheries and Aquatic Science, 44(3): 259-266. https://doi.org/10.5657/KFAS.2011.0259
  17. Maguire, G.B., Boocock, B., Kent, G.N., Gardner, N.C. (1994). Studies on triploid oysters in Australia: IV. Sensory evaluation of triploid and diploid Pacific oysters, Crassostrea gigas (Thunberg), in Tasmania. In: Nell, J.A., Maguire, G.B. (Eds.), Evaluation of triploid Sydney Rock Oysters (Saccostrea commercialis) and Pacific Oysters (Crassostrea gigas) on Commercial Leases in New South Wales and Tasmania, Final Report to FRDC, September, 1994, NSW Fisheries, Port Stephens Research Centre, Taylors Beach, NSW and University of Tasmania, Launceston, Tas., pp. 178-193.
  18. Mugnier, C., Justou, C., Lemonnier, H., Patrois, J., Ansquer, D., Goarant, C. and Lecoz, J.R. (2013). Biological, physiological, immunological and nutritional assessment of farm-reared Litopenaeus stylirostris shrimp affected or unaffected by vibriosis. Aquaculture, 388-391: 105-114. https://doi.org/10.1016/j.aquaculture.2013.01.010
  19. Nell, J.A. (2002). Farming triploid oysters. Aquaculture, 210: 69-88. https://doi.org/10.1016/S0044-8486(01)00861-4
  20. Nell, J.A. and Perkins, B. (2005). Studies on triploid oysters in Australia: farming potential of all triploid Pacific oysters, Crassostrea gigas (Thunberg), in Port Stephens, New South Wales, Australia Aquaculture Research, 36: 530-536. https://doi.org/10.1111/j.1365-2109.2005.01229.x
  21. NFRDI (2012). Research for aquaculture productivity improvement of Pacific oyster, Crassostrea gigas in tidalflat. 2/2 Technical report of NFRDI, 607-624.
  22. Ning, J., Li, C., Yang, G., Wan, A. and Sun, S. (2013). Use of RNA:DNA ratios to evaluate the condition and growth of the copepod Calanus sinicus in the southern Yellow Sea. Deep-Sea Research II. (in progress).
  23. Small, S. A. and Benfey, T.J. (1987). Cell size in triploid salmon. Journal of Experimental Zoology, 241: 339-342. https://doi.org/10.1002/jez.1402410309
  24. Suresh, A.V. and Sheehan, R.J. (1998a). Muscle fiber growth dynamics in diploid and triploid rainbow trout, Oncorhynchus mykiss. Journal of Fish Biology, 52: 570-587. https://doi.org/10.1111/j.1095-8649.1998.tb02018.x
  25. Suresh, A.V. and Sheehan, R.J. (1998b). Biochemical and morphological correlates of growth in diploid and triploid rainbow trout. Journal of Fish Biology, 52: 588-599. https://doi.org/10.1111/j.1095-8649.1998.tb02019.x
  26. Swarup, H. (1959). Effect of triploidy on the body size, general organization and cellular structure in Gasterosteus aculeatus (L.). Journal of Genetics, 56: 143-155. https://doi.org/10.1007/BF02984741
  27. Yang H., Gallivan T., Guo X. and Allen S., 2000. A method for preserving oyster tissue samples for flow cytometry. Journal of shellfish Research, 19: 835-839.
  28. Westerman, M. and Holt, G.J. (1994). RNA:DNA ratio during the critical period and early larval growth of the red drum Sciaenops ocellatus. Marine Biology, 121: 1-9. https://doi.org/10.1007/BF00349468
  29. Wright, D.A. and Hetzel, E.W. (1985). Use of RNA:DNA ratios as an indicator of nutritional stress in the American oyster Crassostrea virginica. Marine Ecological Progress Series, 25: 199-206. https://doi.org/10.3354/meps025199
  30. Writgth, D.A. and Martin, F.D. (1985). The effect of starvation on RNA-DNA ratios and growth of larval striped bass, Morone saxatilis. Journal of Fish Biology, 27(4): 479-485. https://doi.org/10.1111/j.1095-8649.1985.tb03195.x

피인용 문헌

  1. 서해안 굴, Crassostrea gigas의 건강도 평가를 위한 Condition index와 혈구 apoptosis 분석 vol.30, pp.3, 2013, https://doi.org/10.9710/kjm.2014.30.3.189
  2. 통영-거제해역 수하연 양식 참굴(Crassostrea gigas)의 비만도 장기변화와 영향 요인 고찰 vol.54, pp.4, 2013, https://doi.org/10.5657/kfas.2021.0434