한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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TMT 유도성 인지 기능 상실에 대한 대보(밤 품종) 내피 추출물의 효과

Effect of Daebo (Castanea crenata) Inner Skin Extract on TMT-induced Learning and Memory Injury

  • 김현주 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 정지희 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 조유나 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 진동은 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 진수일 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 김만조 (산림과학원 특용자원연구과) ;
  • 허호진 (경상대학교 응용생명과학부, 농업생명과학연구원)
  • Kim, Hyeon-Ju (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Ji Hee (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jo, Yu Na (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jin, Dong Eun (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jin, Su Il (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Man-Jo (Department of Special Purpose Trees, Korea Forest Research Institute) ;
  • Heo, Ho Jin (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University)
  • 투고 : 2013.04.15
  • 심사 : 2013.06.30
  • 발행 : 2013.10.31
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초록

본 연구에서는 국내 대표 밤 품종인 대보(Daebo)의 내피를 활용하여 TMT 유도성 인지 기능 상실에 대한 개선 효과를 연구하였다. 실험에서 각 농도별(5, 10, 20 mg/kg of body weight)로 대보 내피 에틸아세테이트 분획물을 섭취한 mouse를 TMT로 인지기능 손상을 유발하여 Y-maze test와 passive avoidance test한 결과, Y-maze test에서 분획물을 섭취한 group이 TMT 단독 처리군과 비교하였을 때 공간 인지기능을 개선시켰고, passive avoidance test 또한 latency time이 증가한 것으로 나타나 TMT에 의해 유발되는 뇌 신경독성 동물 모델로부터 기억 및 학습능력 개선 효과를 갖는 것으로 확인되었다. 또한 in vivo 동물 실험 후 mouse로부터 적출된 whole brain tissue를 대상으로 ex vivo AChE 활성 및 MDA 함량측정 실험한 결과, 에틸아세테이트 분획물이 TMT효과 대비 AChE의 활성을 일부 유의적으로 억제하는 것을 알 수 있었다. 결론적으로 대보 내피 에틸아세테이트 분획물은 신경전달물질인 AChE의 활성을 저해하고 뇌 신경세포 보호 효과를 통하여 인지기능 개선 효과를 유도할 수 있는 것으로 사료된다.

The aim of this study was to investigate the anti-amnesic effect of daebo (Castanea crenata) extract on trimethyltin chloride (TMT)-induced learning and memory impairment, in vivo. The inner skin of daebo was extracted using distilled water under reflux conditions. At the end of the adaptation period, ICR mice were divided into a control group, a TMT injection group (negative control), and a sample group (C5: 5 mg/kg body weight; C10: 10 mg/kg body weight; and C20: 20 mg/kg body weight), and were tested with learning and memory tests. The ethylacetate fraction of the daebo inner skin extract was found to increase TMT-induced memory deficit in the Y-maze and passive avoidance test. Brain tissue analysis showed that the ethylacetate fraction of daebo extract lowered the acetylcholine esterase (AChE) activity and malondialdehyde (MDA) content of neuronal cells, both of which are indicative of lipid peroxidation.

키워드

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