한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제26권5호
  • /
  • Pages.613-618
  • /
  • 2013
  • /
  • 1226-3591(pISSN)
  • /
  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

희귀수종 먹넌출 엽의 형태적 특성과 유전변이

Characteristics of Leaf Morphology and Genetic Variation of the Rare Woody Plant, Berchemia racemosa var. magna

  • 송정호 (국립산림과학원 산림유전자원부 특용자원연구과) ;
  • 임효인 (국립산림과학원 산림유전자원부 산림유전자원과) ;
  • 장경환 (국립산림과학원 산림유전자원부 산림유전자원과) ;
  • 한진규 (국립산림과학원 산림유전자원부 산림유전자원과)
  • Song, Jeong-Ho (Division of Special-purpose Trees, Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Lim, Hyo-In (Division of Forest Genetic Resources, Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Jang, Kyung-Hwan (Division of Forest Genetic Resources, Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Han, Jingyu (Division of Forest Genetic Resources, Department of Forest Genetic Resources, Korea Forest Research Institute)
  • 투고 : 2013.04.11
  • 심사 : 2013.10.16
  • 발행 : 2013.10.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

우리나라에서 안면도에만 분포하는 먹넌출 집단의 생명자원보존을 위하여 엽의 형태적 특성과 I-SSR 표지자를 이용한 유전변이를 조사하였다. 10가지 엽특성에 대한 ANOVA 분석결과 모든 특성에서 개체 간에 통계적인 유의성이 인정되었다. 조사된 39개체의 평균특성은 엽신장 11.8 cm, 최대엽폭 7.1 cm, 엽지수 1.67, 상1/3폭 5.4 cm, 하1/3폭 6.2 cm, 엽병길이 3.6 cm, 엽두께 0.19 mm, 엽맥수(좌) 11.5개, 엽맥수(우) 11.4개, 엽면적 61.7 $cm^2$로 나타났다. 변이계수 값은 엽두께, 엽병길이, 엽면적이 각각 18.8%, 21.7%, 22.0%로 높게 나타났으며, 나머지 특성들에서는 15% 이내의 비교적 낮은 변이를 나타냈다. 선발된 8개 I-SSR Primer에서 총 50개의 증폭산물을 얻었으며, 유효대립 유전자의 수 1.719개, 다형적 유전자좌의 비율 26.0%, 이형접합도의 기대치 0.410 및 Shannon의 다양성지수 0.598로 각각 나타났다. 안면도 먹넌출 집단은 제한된 지역에 분포하며 개체수가 적음에도 불구하고 높은 유전다양성을 유지하는 것으로 나타났다.

This study was conducted to investigate the morphological characteristics of leaf and the genetic diversity of Berchemia racemosa var. magna which is only found in Anmyeon Island of South Korea. ANOVA test showed that there were significant differences among individuals within population in all 10 leaf characteristics. Average characteristics of 39 individuals were 11.8 cm in leaf length, 7.1 cm in leaf width, 1.67 in leaf index, 5.4 cm in upper 1/3 width, 6.2 cm in lower 1/3 width, 3.6 cm in petiole length, 0.19 mm in leaf thickness, 11.5 ea. in number of veins (left), 11.4 ea. in number of veins (right) and 61.7 $cm^2$ in leaf area, respectively. Except for leaf thickness (18.8%), petiole length (21.7%) and leaf area (22.0%), the coefficients of variation of most leaf characteristics were relatively low (<15.0%). A total of 50 bands was generated from 8 selected I-SSR primers. The estimates of genetic variation were 1.719 in effective number of alleles ($A_e$), 26.0% in proportion of polymorphic bands (P), 0.410 in expected heterozygosity ($H_e$) and 0.598 in Shannon's diversity index (S.I.), respectively. In spite of the small number and the limited distribution, the B. racemosa var. magna population in Anmyeon Island showed high genetic diversity.

키워드

참고문헌

  1. Ahn, Y.S., S.H. Kim, H.K. Jung, Y.S. Jang, Y.C. Choi and K.I. Oh. 2002. The variation of leaf characters among natural populations of Kalopanax septemlobus Koidz. Jour. Korean For. Soc. 91(6):755-764.
  2. Baali-Cherif, D. and G. Besnard. 2005. High genetic diversity and clonal growth in relict populations of Olea europaea subsp. laperrinei (Oleaceae) from Hoggar, Algeria. Ann. Bot. London 96(5):823-830. https://doi.org/10.1093/aob/mci232
  3. Beon, M.S. and Y.H. Kim. 2008. Vegetation structure and population dynamics of Berchemia racemosa habits. Kor. J. Env. Eco. 22(6):679-690 (in Korean).
  4. Chen, T.L. and S. Carsten. 2007. Berchemia. In Wu, Z.Y., P.H. Raven and D.Y. Hong (eds.), Flora of China, Vol. 12, Science Press, Beijing and Missouri Botanical Garden Press, St. Louis, USA. pp. 124-130.
  5. Choi, H.S., K.N. Hong, J.M. Chung, B.Y. Kang and W.W. Kim. 2004. Genetic diversity and spatial genetic structure of Empetrum nigrum var. japonicum in Mt. Halla, South Korea. Jour. Korean For. Soc. 93(3):175-180 (in Korean).
  6. Hamrick, J.L. M.J.W. Godt and S.L. Sherman-Broyles. 1992. Factors influencing levels of genetic diversity in woody plant species. New Forests 6(1):95-124. https://doi.org/10.1007/BF00120641
  7. Hong, Y.P., H.Y. Kwon, K.S. Kim, K.N. Hong and Y.Y. Kim. 2004. Discordance between geographical distribution and genetic relationship among populations of Japanese red pine in Korea revealed by analysis of I-SSR markers. Silvae Genet. 53(3):89-92.
  8. Jeong, J.H., K.S. Kim, C.H. Lee and Z.S. Kim. 2007. Genetic diversity and spatial structure in populations of Abelia tyaihyoni. Jour. Korean For. Soc. 96(6):667-675 (in Korean).
  9. Ji, D.H., Y.T. Kim, K.N. Kang, Y.Y. Lee and S.H. Cho. 2012. A composition comprising extract of Berchemia racemosa var. magna having antioxidant and whitening effect. Korea Intellectual Property Rights 10-2011-0083528 (in Korean).
  10. Jump, A.S., R. Marchant and J. Penuelas. 2008. Environmental change and the option value of genetic diversity. Trends Plant Sci. 14(1):51-58.
  11. Kang, B.Y., K.N. Hong, J.M. Chung and Y.P. Hong. 2003. Spatial genetic structure of Korean black raspberry (Rubus coreanus) at Mt. Chiak using I-SSR markers. Jour. Korean For. Soc. 92(6):558-566 (in Korean).
  12. Kim, S.Y., Y.D. Kim, J.S. Kim, B.H. Yang, S.H. Kim and B.C. Lee. 2009. Genetic diversity of Forsythia ovata Nakai (Oleaceae) based on inter-simple sequence repeats (ISSR). Korean J. Pl. Taxon 39(1):48-54 (in Korean).
  13. Kim, Y.J., K.C. Kim, B.S. Lee, G.Y. Lee, K.J. Cho, J.T. Kang and T.D. Kim. 2005. The variation of leaf characteristics in 6 natural populations of Stewartia koreana Nakai. Jour. Korean For. Soc. 94(6):446-452 (in Korean).
  14. Kimura, M. and J.F. Crow. 1964. The number of alleles that can be maintained in a finite population. Genetics 49(4):725-738.
  15. KNA (Korea National Arboretum). 2008. Rare Plants Data Book in Korea. GEOBOOK, Seoul, Korea. p. 186 (in Korean).
  16. Koidzumi, G. 1916. Decades plantarum novarum vel minus cognitarum. Bot. Mag. Tokyo 30:325-326. https://doi.org/10.15281/jplantres1887.30.325
  17. Lee, T.B. 2003. Coloured Flora of Korea. Hyangmoonsa Publishing Co., Seoul, Korea. p. 716.
  18. Lewontin, R.C. 1972. The apportionment of human diversity. Evol. Biol. 6:381-398.
  19. Lynch, M., J. Conery and R. Burger. 1995. Mutation accumulation and the extinction of small populations. Am. Nat. 146(4):489-518. https://doi.org/10.1086/285812
  20. Makino, T. 1892. Notes on Japanese plants X VI. Bot. Mag. Tokyo 6:169-170 (in Japanese). https://doi.org/10.15281/jplantres1887.6.169
  21. Moon, A.R., H.J. Kim, J.M. Park, S.H. Kang and C.G. Jang. 2012. The study for the flora of 6 islands area in the western sea of Chungnam province. Korean J. Plant Res. 25(1):105-122 (in Korean). https://doi.org/10.7732/kjpr.2012.25.1.105
  22. Pearman, P., A. Guisan., O. Broenninmann and C.F. Randin. 2007. Niche dynamics in space and time. Trends in Ecology and Evolution 23(3):149-158.
  23. SAS Institute Inc. 1999. SAS/STAT user's guide, version 8.01. SAS Institute Inc., Cary, North Carolina, USA.
  24. Shaffer, M.L. 1981. Minimum population sizes for species conservation. BioScience 31(2):31-134.
  25. Song, J.H., M.H. Park, H.K. Moon, S.U. Han and J.S. Yi. 2000. The variation of leaf form of natural populations of Quercus variabilis in Korea. Jour. Korean For. Soc. 89(5):666-676 (in Korean).
  26. Song, J.H., J.J. Lee, K.S. Kang and S.D. Hur. 2008. The variation of leaf form of rare endemic Berchemia berchemiaefolia populations. Jour. Korean For. Soc. 97(4):431-436.
  27. Song, J.H., K.H. Jang, B.H. Yang and H.I. Lim. 2011. Propagation method of natural monument Berchemia racemosa var. magna Makino using hardwood cutting. Korea Intellectual Property Rights 10-2011-0026543 (in Korean).
  28. Song, J.H., H.I. Lim, K.N. Hong, K.H. Jang and Y.P. Hong. 2012. Genetic diversity and spatial genetic structure of dwarf stone pine in Daecheongbong Area, Mt. Seorak. Korean J. Plant Res. 25(4):407-415 (in Korean). https://doi.org/10.7732/kjpr.2012.25.4.407
  29. Tsukaya, H. 2005. Leaf shape: genetic controls and environmental factors. Int. J. Dev. Biol. 49(5/6):547-555. https://doi.org/10.1387/ijdb.041921ht
  30. UNEP. 2010. Conference of Parties to the Convention on Biological Diversity. Decision X/17. Consolidated update of the Global Strategy for Plant Conservation 2011-2020. (2013. 4. 5).
  31. Wang, Y.F., J.X. Cao, T. Efferth, G.F. Lai and S.D. Luo. 2006. Cytotoxic and new tetralone derivatives from Berchemia floribunda (Wall.) Brongn. Chem. Biodivers. 3(6):646-653. https://doi.org/10.1002/cbdv.200690067
  32. Wolfe, A.D. 2005. ISSR techniques for evolutionary biology. Methods Enzymol. 395: 134-144. https://doi.org/10.1016/S0076-6879(05)95009-X
  33. Yeh, F.C., R.C. Yang, T.B.J. Boyle, Z.H. Ye and J.X. Mao. 1997. POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta. Edmonton, Canada.
  34. Zietkiewicz, E., A. Rafalski and D. Labuda. 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20(2):176-183. https://doi.org/10.1006/geno.1994.1151
  35. 송정호. 2009. 희귀 및 멸종위기수종 먹넌출의 유전자원 보존. 월간산림 5월. pp. 88-89.

피인용 문헌

  1. Seasonal Variation in Photosynthetic Characteristics and Chlorophyll Content of the Loranthus tanakae, Viscum album var. coloratum and its Hosts in Korea vol.104, pp.1, 2015, https://doi.org/10.14578/jkfs.2015.104.1.50
  2. 인간 대장암 세포에 대한 먹넌출 추출물의 GSK3β 의존성 threonine-286 인산화를 통한 Cyclin D1 분해 vol.33, pp.4, 2013, https://doi.org/10.7732/kjpr.2020.33.4.271
  3. Anti-Inflammatory Effects of Berchemia floribunda in LPS-Stimulated RAW264.7 Cells through Regulation of NF-κB and MAPKs Signaling Pathway vol.10, pp.3, 2013, https://doi.org/10.3390/plants10030586