Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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A Comparative Study of Physiological Activity of Glycyrrhiza uralensis Fischer Stems and Leaves by Processing Methods

감초 경엽(莖葉)의 포제방법에 따른 생리활성 비교

  • Park, Jeong Seob (Korea Agricultural Management Technique Institute) ;
  • Park, Sun Hee (Department of Oriental Medicine Resources, Chonbuk National University) ;
  • Oh, Il Soo (Department of Oriental Medicine Resources, Chonbuk National University) ;
  • Chang, Young Nam (Department of Oriental Medicine Resources, Chonbuk National University) ;
  • Bang, Keuk Soo (Department of Oriental Medicine Resources, Chonbuk National University) ;
  • Byeon, Eun Ju (Department of Oriental Medicine Resources, Chonbuk National University) ;
  • Lee, Jeong Ho (Department of Oriental Medicine Resources, Chonbuk National University)
  • 박정섭 (한국농업경영기술연구원) ;
  • 박선희 (전북대학교 한약자원학과) ;
  • 오일수 (전북대학교 한약자원학과) ;
  • 장영남 (전북대학교 한약자원학과) ;
  • 방극수 (전북대학교 한약자원학과) ;
  • 변은주 (전북대학교 한약자원학과) ;
  • 이정호 (전북대학교 한약자원학과)
  • Received : 2013.08.30
  • Accepted : 2013.10.28
  • Published : 2013.10.31
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Abstract

This study investigates the change of chemical components, antioxidant and antimutagenic activity in Glycyrrhiza uralensis Fischer stems and leaves (GU) by the various processing methods, as follows: fresh (GU-1), dried under the shade (GU-2), blanched (GU-3), roasted 3 times (GU-4), roasted 4 times (GU-5). The components have been identified and quantified through the use of gas chromatograph-mass spectrometry (GC-MS). At results, cis-1,3-dimethyl-2-methylenecyclohexaneat at 19.7 min and n-hexadecanoic acid at 21.5 min were detected in five kinds of extract. 3-O-Methyl-D-fructose at 17.8 min was observed in four extracts except in GU-1 and aminopyrazine at 19.8min was observed in four extracts except in GU-4. The total phenolics contents was high in GU-4(3.38 g/100 g). ABTS radical scavenging was high in GU-5($EC_{50}$, 0.57 ug) and DPPH radical scavenging was high in GU-4($EC_{50}$, 2.66 ug). The extracts of GU-3, GU-4, and GU-2 were most potent in anti-mutagenicity activity against 1-NP, Trip-P-1, and Trip-P-2, respectively. GU-3 and GU-4 also showed most potent effect of anti-mutagenicity on 2-AA and AFB1, respectively.

포제 방법에 따른 감초경엽의 성분변화 및 항산화, 항돌연변이원성을 평가하였다. 성분변화를 관찰하기 위해 GC-Mass의 분석한 결과 각 추출물에서 공통으로 분석된 성분은 19.7분에서 cis-1,3-dimethyl-2-methylene-cyclohexane과 21.5에서 n-hexadecanoic acid이 모두 분석되었으며, 17.8분에서는 3-O-methyl-D-Fructose가 끓는 물에 데친 감초경엽에서 19.8분에서는 aminopyrazine이 4회 볶은 감초 잎과 줄기 추출물만 분석되지 않고 4가지 모두에서 분석되었다. 항산화 활성 측정에서는 DPPH 라디칼 소거활성에서 3회 볶은 감초경엽에서 $EC_{50}$이 $2.66{\pm}0.09{\mu}g$으로 가장 높은 활성을 나타났으며, ABTS 라디칼 소거활성은 4회 볶은 감초경엽에서 $EC_{50}$이 $0.57{\pm}0.22{\mu}g$으로 가장 높았다. 환원력 측정에서는 3회 볶은 감초 잎과 줄기 추출물에서 가장 높게 나타났으며, 폴리페놀 함량 3회 볶은 감초경엽에서 $3.38{\pm}0.12g/100g$으로 가장 높았다. 항돌연변이원성 평가에 있어서 직접변이원인 1-NP에 대해 높은 돌연변이 억제효과를 나타내었으며, 간접변이원인인 Trp-P-1, Trp-P-2, $AFB_1$, 2-AA에 대해서는 3번 볶은 감초경엽(GU-1)에서 가장 높은 돌연변이 억제효과를 나타냈다.

Keywords

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