한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제58권3호
  • /
  • Pages.283-291
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  • 2013
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  • 0252-9777(pISSN)
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  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
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남일벼 돌연변이 후대 계통 'Namil(SA)-flo1'의 분질배유 특성에 대한 유전분석

Genetic Analysis on Floury Endosperm Characteristics of 'Namil(SA)-flo1', a Japonica Rice Mutant Line

  • 투고 : 2013.04.22
  • 심사 : 2013.08.05
  • 발행 : 2013.09.30
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초록

쌀 가공산업을 활성화하고 소비를 촉진하여 국내 쌀 생산 기반을 유지하기 위해서는 쌀가루 제분적성을 보유한 가공용 벼 품종 개발이 시급하다. 농촌진흥청 국립식량과학원에서는 아지드화나트륨을 돌연변이원으로 활용하여 건식제분 적합성을 보유한 분질배유 돌연변이 후대계통인 'Namil(SA)-flo1'를 육성한 바 있다. 본 연구는 염색체 상에서 'Namil(SA)-flo1'의 분질배유 특성을 지배하는 유전자위를 탐색하고자 수행하였다. 주요 결과는 아래와 같다. 1. 'Namil(SA)-flo1' ${\times}$ '밀양23호'로부터 유래한 F2 94 개체로부터 종자 분질립 비율을 검정하고 54개 SSR 마커의 유전자형을 검정하여 연관성분석(association analysis)을 수행한 결과 목표 유전자위는 5번 염색체 중하단 부위로 추정되었다. 2. 목표 부위의 SSR 마커 밀도를 높여 추가 연관성분석을 실시하였고, F2:3 종자 분질립 변이의 79.7%가 5번 염색체 상의 RM164의 유전자형 변이에 의하여 설명된다는 것을 확인하였다. 3. 이를 통하여 분질배유 지배 유전자위를 5번 염색체 17.7~20.7 Mbp 부위로 추정하였으며, 추후 추가 분리집단을 이용하여 목표 유전자를 동정하고 쌀가루용 품종 개발에 활용할 수 있는 핵산정밀표지인자를 개발할 계획이다.

Rice varieties with suitable flour-making quality are required to promote rice processed-food industry and boost rice consumption in Korea. 'Namil (SA)-flo1' is an advanced mutant line with floury endosperm which shows good flour-making quality under dry-milling process. Genetic analysis was carried out to localize the chromosomal region responsible for the floury endosperm of 'Namil (SA)- flo1'. By using 94 F2 progenies, which were derived from 'Namil (SA)-flo1' ${\times}$ 'Milyang 23', floury grains percentage was investigated as phenotypic data, and genotyping was conducted with 54 SSR markers. Association analysis showed that the target genetic region for floury endosperm is on middle-low region of chromosome 5. Through further association analysis with increased number of SSR markers on chromosome 5, we found that genotypic variation in RM164 explains 79.7% of the variation in floury grains percentage of F2:3 seeds. The floury endosperm locus was localized on 17.7-20.7 Mbp region of chromosome 5 and will be further analyzed for fine mapping and gene identification.

키워드

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피인용 문헌

  1. Agronomic and Genetic Evaluation on a Dull Mutant Line Derived from the Sodium Azide Treated ‘Namil’, a Non-Glutinous Japonica Rice vol.60, pp.4, 2015, https://doi.org/10.7740/kjcs.2015.60.4.448
  2. A New SNP in Rice Gene Encoding Pyruvate Phosphate Dikinase (PPDK) Associated with Floury Endosperm vol.11, pp.4, 2013, https://doi.org/10.3390/genes11040465