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삼지구엽초 추출물의 항균 및 항산화 활성에 대한 연구

Antibacterial and Antioxidative Activities of Epimedium koreanum Nakai Extracts

  • 원두현 (서울과학기술대학교 정밀화학과, 나노바이오화장품연구실, 화장품종합기술연구소) ;
  • 구현아 (서울과학기술대학교 정밀화학과, 나노바이오화장품연구실, 화장품종합기술연구소) ;
  • 김혜진 (서울과학기술대학교 정밀화학과, 나노바이오화장품연구실, 화장품종합기술연구소) ;
  • 한샛별 (서울과학기술대학교 정밀화학과, 나노바이오화장품연구실, 화장품종합기술연구소) ;
  • 박진오 (대봉엘에스(주)) ;
  • 박수남 (서울과학기술대학교 정밀화학과, 나노바이오화장품연구실, 화장품종합기술연구소)
  • Won, Doo Hyun (Department of Fine Chemistry, Nanobiocosmetic Laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Gu, Hyun A (Department of Fine Chemistry, Nanobiocosmetic Laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Kim, Hye Jin (Department of Fine Chemistry, Nanobiocosmetic Laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Han, Saet Byeol (Department of Fine Chemistry, Nanobiocosmetic Laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Park, Jino (DAEBONG LS) ;
  • Park, Soo Nam (Department of Fine Chemistry, Nanobiocosmetic Laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology)
  • 투고 : 2012.12.18
  • 심사 : 2013.05.21
  • 발행 : 2013.09.28

초록

본 연구에서는 화장품 원료로서 삼지구엽초의 응용가능성을 확인하기 위해 항균 효과와 항산화 효과를 확인하였다. 피부상재균에 대한 disc diffusion assay에서 높은 항균 효과를 나타낸 분획-균에 대해 최소저해농도를 측정하였다. Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis), Propionibacterium acnes (P. acnes)에 대한 에틸아세테이트 분획의 항균 활성과, S. aureus에 대한 50% 에탄올 분획의 항균 활성은 대표적인 방부제인 methyl paraben보다 우수한 것으로 확인되었다. DPPH (1,1-diphenyl-2-picrylhydrazyl)를 이용한 라디칼 소거 활성에서는 삼지구엽초의 세 분획 모두 대표적 항산화제인 (+)-${\alpha}$-토코페롤보다 낮은 것으로 나타났다. $Fe^{3+}-EDTA/H_2O_2$ 계를 이용해 다양한 ROS에 대한 총 항산화 활성($OSC_{50}$)을 실험한 결과, 50% 에탄올 추출물($OSC_{50}=2.46{\pm}0.06{\mu}g/ml$)과 아글리콘 분획($OSC_{50}=1.45{\pm}0.02{\mu}g/ml$)은 비교 물질인 L-ascorbic acid($OSC_{50}=1.50{\pm}0.85{\mu}g/ml$)과 유사한 높은 활성을 보여주었다. 광증감 반응을 이용한 세포 보호 효과(${\tau}_{50}$) 실험에서 50% 에탄올 추출물(${\tau}_{50}=37.0{\pm}0.3$ min)은 비교 물질인 (+)-${\alpha}$-토코페롤(${\tau}_{50}=38.0{\pm}1.8$ min)과 유사한 값을 보여주었다. 특히 아글리콘 분획의 ${\tau}_{50}$$165.9{\pm}7.2$ min으로 비교 물질인 (+)-${\alpha}$-토코페롤에 비해 4배가 넘는 높은 세포 보호 효과를 나타내었다. 이러한 결과들을 종합해보았을 때, 삼지구엽초 추출물은 항균 또는 항산화 활성을 갖는 화장품 원료로서 응용가능성이 높은 것으로 확인하였다.

In this study, the antibacterial and antioxidative activities of Epimedium koreanum Nakai were investigated for applications as cosmetic ingredients. Minimum inhibitory concentrations (MICs) of fraction-bacterium, that showed high antibacterial activity from disc diffusion assay on human skin pathogens, were tested. The ethyl acetate fraction on Staphylococcus aureus, Bacillus subtilis, Propionibacterium acnes and 50% ethanol extract on S. aureus exhibited higher antibacterial activities than methyl paraben, well known as a preservative. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities of 3 fractions of E. koreanum Nakai were lower than (+)-${\alpha}$-tocopherol, known as a typical antioxidant. From the results of the scavenging activities of various ROS generated in $Fe^{3+}-EDTA/H_2O_2$ systems ($OSC_{50}$), 50% ethanol extract ($OSC_{50}=2.46{\pm}0.06{\mu}g/ml$) and aglycone fraction ($OSC_{50}=1.45{\pm}0.02{\mu}g/ml$) showed high activities similar to L-ascorbic acid ($OSC_{50}=1.50{\pm}0.85{\mu}g/ml$), used as reference. The cellular protective effects (${\tau}_{50}$) on photohemolysis by $^1O_2$ generated by photosensitization reaction were tested. The cellular protective effect of 50% ethanol extract (${\tau}_{50}=37.0{\pm}0.3$ min) was similar to (+)-${\alpha}$-tocopherol (${\tau}_{50}=38.0{\pm}1.8$ min), used as reference. In particular, the ${\tau}_{50}$ of aglycone fraction results were $165.9{\pm}7.2$ min. This is a high cellular protective effect, more than 4 times that of (+)-${\alpha}$-tocopherol. These results indicate that E. koreanum Nakai extract, and its fractions, could be utilized as a cosmetic ingredient possessing antibacterial and antioxidative activities.

키워드

참고문헌

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