Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Growth and Cyanide Degradation of Azotobacter vinelandii in Cyanide-Containing Wastewater System

  • Koksunan, Sarawut (Department of Biotechnology, Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University) ;
  • Vichitphan, Sukanda (Department of Biotechnology, Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University) ;
  • Laopaiboon, Lakkana (Department of Biotechnology, Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University) ;
  • Vichitphan, Kanit (Department of Biotechnology, Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University) ;
  • Han, Jaehong (Metalloenzyme Research Group and Department of Biotechnology, Chung-Ang University)
  • Received : 2012.09.10
  • Accepted : 2012.12.29
  • Published : 2013.04.28
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Abstract

Azotobacter vinelandii, a strict aerobic nitrogen-fixing bacterium, has been extensively studied with regard to the ability of $N_2$-fixation due to its high expression of nitrogenase and fast growth. Because nitrogenase can also reduce cyanide to ammonia and methane, cyanide degradation by A. vinelandii has been studied for the application in the bioremediation of cyanide-contaminated wastewater. Cyanide degradation by A. vinelandii in NFS (nitrogen-free sucrose) medium was examined in terms of cell growth and cyanide reduction, and the results were applied for cyanide-contaminated cassava mill wastewater. From the NFS medium study in the 300 ml flask, it was found that A. vinelandii in the early stationary growth phase could reduce cyanide more rapidly than the cells in the exponential growth phase, and 84.4% of cyanide was degraded in 66 h incubation upon addition of 3.0 mM of NaCN. The resting cells of A. vinelandii could also reduce cyanide concentration by 90.4% with 3.0 mM of NaCN in the large-scale (3 L) fermentation with the same incubation time. Finally, the optimized conditions were applied to the cassava mill wastewater bioremediation, and A. vinelandii was able to reduce the cyanide concentration by 69.7% after 66 h in the cassava mill wastewater containing 4.0 mM of NaCN in the 3 L fermenter. Related to cyanide degradation in the cassava mill wastewater, nitrogenase was the responsible enzyme, which was confirmed by methane production. These findings would be helpful to design a practical bioremediation system for the treatment of cyanide-contaminated wastewater.

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References

  1. Adjei, M. D. and Y. Ohta. 2000. Factors affecting the biodegradation of cyanide by Burkholderia cepacia strain C-3. J. Biosci. Bioeng. 89: 274-277.
  2. APHA, AWWA, & WPCF. 1995. Standard Methods for the Examination of Water and Wastewater. 19th Ed. In A. D. Enton, L. S. Uesceri, and A. E. Greenberg (eds.). American Public Health Association, Washington, DC.
  3. Baxter, J. and S. P. Cummings. 2006. The current and future applications of microorganism in the bioremediation of cyanide contamination. Antonie Van Leeuwenhoek 90: 1-7. https://doi.org/10.1007/s10482-006-9057-y
  4. Burgess, B. K. and D. J. Lowe. 1996. Mechanism of molybdenum nitrogenase. Chem. Rev. 96: 2983-3011. https://doi.org/10.1021/cr950055x
  5. Drochioiu, G. 2002. Fast and highly selective determination of cyanide with 2,2-dihydroxy-1,3-indanedione. Talanta 56: 1163- 1165. https://doi.org/10.1016/S0039-9140(01)00609-9
  6. Ehaliotis, C., K. Papadopoulou, M. Kotsou, I. Mari, and C. Balis. 1999. Adaptation and population dynamics of Azotobacter vinelandii during aerobic biological treatment of olive-mill wastewater. FEMS Microbiol. Ecol. 30: 301-311. https://doi.org/10.1111/j.1574-6941.1999.tb00658.x
  7. Eisler, R. 1991. Cyanide hazards to fish, wildlife and invertebrates. Biological Report Vol 85. U.S. Department of the Interior, Fish and Wildlife Service, Washington, DC.
  8. Gapes, D. J., N. M. Frost, T. A. Colark, P. Dare, R. G. Hunter, and A. H. Slade. 1999. Nitrogen fixation in treatment of pulp and paper wastewaters. Water Sci. Technol. 40: 85-92.
  9. Han, J. and W. E. Newton. 2004. Differentiation of acetylenereduction sites by stereoselective proton addition during Azotobacter vinelandii nitrogenase-catalyzed $C_2D_2$ reduction. Biochemistry 43: 2947-2956. https://doi.org/10.1021/bi035247y
  10. Kaewkannetra, K., T. Imai, F. J. Garcia-Garcia, and T. Y. Chiu. 2009. Cyanide removal from cassava mill wastewater using Azotobactor vinelandii TISTR 1094 with mixed microorganisms in activated sludge treatment system. J. Hazard. Mater. 172: 224-228. https://doi.org/10.1016/j.jhazmat.2009.06.162
  11. Kao, C. M., J. K. Liu, H. R. Lou, C. S. Lin, and S. C. Chen. 2003. Biotransformation of cyanide to methane and ammnonia by Klebsiella oxytoca. Chemosphere 50: 1055-1061. https://doi.org/10.1016/S0045-6535(02)00624-0
  12. Kim, M. and J. Han. 2007. Synthesis, structure and physical properties of trinuclear $ M_3tdt_3(PEt_3)_3$ (M=$Fe^{II}$, $Co^{II}$) clusters containing metal-metal bonds. Polyhedron 26: 4557-4566. https://doi.org/10.1016/j.poly.2007.06.011
  13. Li, J., B. K. Burgess, and J. L. Corbin. 1982. Nitrogenase reactivity: Cyanide as substrate and inhibitor. Biochemistry 21: 4393-4402. https://doi.org/10.1021/bi00261a031
  14. Newton, W. E. 1993. Nitrogenases: Distribution, composition, structure and function, pp. 5-18. In R. Palacios, J. Mora, and W. E. Newton (eds.). New Horizons in Nitrogen Fixation. Kluwer Academic, Dordrecht, The Netherlands.
  15. Shin, B. K. and J. Han. 2008. Structure and physical properties of copper thiomolybdate complex, $(^{n}Bu_{4}N)_{3}[MoS_{4}Cu_{3}Cl_{4}]$. Bull. Korean Chem. Soc. 29: 2299-2302. https://doi.org/10.5012/bkcs.2008.29.11.2299
  16. Stranberg, G. W. and P. W. Wilson. 1968. Formation of the nitrogen-fixing enzyme system in Azotobacter vinelandii. Can. J. Microbiol. 14: 25-31. https://doi.org/10.1139/m68-005
  17. Watanabe, A., K. Yano, K. Ikebukuro, and I. Karube. 1998. Cyanide hydrolysis in a cyanide-degrading bacterium, Pseudomonas stutzeri AK61, by cyanidase. Microbiology 144: 1677-1682. https://doi.org/10.1099/00221287-144-6-1677
  18. White, J. M., D. D. Jones, D. Huang, and J. J. Gauthier. 1988. Conversion of cyanide to formate and ammonia by Pseudomonas from industrial wastewater. J. Ind. Microbiol. Biotechnol. 3: 263-272.

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