Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Synthesis of NBD-Labeled DOTAP Analog to Track Intracellular Delivery of Liposome

  • Doh, Kyung-Oh (Department of Physiology, College of Medicine, Yeungnam University) ;
  • Kim, Bieong-Kil (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Lee, Tae-Jin (Department of Anatomy, College of Medicine, Yeungnam University) ;
  • Park, Jong-Won (Department of Internal Medicine, College of Medicine, Yeungnam University) ;
  • Seu, Young-Bae (School of Life Sciences and Biotechnology, Kyungpook National University)
  • Received : 2012.08.31
  • Accepted : 2012.11.12
  • Published : 2013.01.28
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Abstract

A DOTAP analog labeled by NBD on the head group (DTNBD) was designed and synthesized to label DOTAP liposome. The structure was confirmed by $^1H$ NMR and FAB-MS, and the fluorescence of the newly synthesized DT-NBD was observed by fluorescent microscopy. The transfection efficiency of DOTAP liposome containing DT-NBD was comparable to commonly used NBD PE in COS7 and MCF7 cells. Furthermore, the level of cellular uptake and fluorescent intensity of fluorescent liposome containing DT-NBD was higher than NBD PE. Therefore, the novel NBD-labeled DOTAP analog seems to be effectively used for investigation of the cellular interaction and transfection mechanism of DOTAP liposome.

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References

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