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Unanticipated Gene Deletion in the Transgenic Chicken Employing Ovalbumin Promoter for Oviduct Specific Expression

  • Jang, Tae Young (Department of Zootechnical Science, KyungPook National University) ;
  • Koo, Bon Chul (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Kwon, Mo Sun (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Roh, Ji Yeol (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Kim, Teoan (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Park, Young Sik (Department of Zootechnical Science, KyungPook National University)
  • Received : 2013.08.28
  • Accepted : 2013.09.05
  • Published : 2013.09.30

Abstract

Transgenic chickens have been spotlighted as an highly potent bioreactor for their fecundity, short generation time, and eggs associated with mass production of protein. In this study, we generated transgenic chickens exhibiting oviduct specific expression of human growth hormone fused to human transferrin for oral administration. Gene of the modified growth hormone located at downstream ovalbumin promoter (~3.6 kb) was introduced to stage X blastodermal cell employing retrovirus vector system. Several transgenic chickens were successfully generated. However, genomic analyses showed unexpected deletion within the transgene. The modification of the transgene seemed to occur during germ cell formation because the deletion was detected only from the sperm DNA of the G0 founder animal. There was no evidence of deletion in the somatic cell DNA samples of the same chicken. Consequently, same pattern of the deletion was confirmed in both somatic and germ cells of the G1 progeny.

Keywords

References

  1. Amet N, Lee HF, Shen WC (2009): Insertion of the designed helical linker led to increased expression of tf-based fusion proteins. Pharm Res 26:523-528. https://doi.org/10.1007/s11095-008-9767-0
  2. Amet N, Wang W, Shen WC (2010): Human growth hormone-transferrin fusion protein for oral delivery in hypophysectomized rats. J Control Release 141:177-182. https://doi.org/10.1016/j.jconrel.2009.09.007
  3. Eyal-Giladi H, Kochav S (1976): From cleavage to primitive streak formation: A complementary normal table and a new look at the first stages of the development of the chick. Dev Biol 49:321-337. https://doi.org/10.1016/0012-1606(76)90178-0
  4. Ivarie R (2003): Avian transgenesis: progress towards the promise. Trends Biotechnol 21:14-19. https://doi.org/10.1016/S0167-7799(02)00009-4
  5. Kamihira M, Ono KI, Esaka K, Nishijima KI, Kigaku R, Komatsu H, Yamashita T, Kyogoku K, Iijima S 2005 High-level expression of single-chain Fv- Fc fusion protein in serum and egg white of genetically manipulated chickens by using a retroviral vector. J Virol 79:10864-10874. https://doi.org/10.1128/JVI.79.17.10864-10874.2005
  6. Kim T (2002): Retrovirus-mediated gene transfer. In: Pinkert CA (eds.). Transgenic Animal Technology. 2nd ed. Academic Press, San Diego, CA, pp 173- 193.
  7. Koo BC, Kwon MS, Lee H, Kim M, Kim D, Roh JY, Park Y-Y, Cui XS, Kim NH, Byun SJ, Kim T (2010): Tetracycline-dependent expression of the human erythropoietin gene in transgenic chickens. Transgenic Res 19:437-447. https://doi.org/10.1007/s11248-009-9327-3
  8. Lillico SG, Sherman A, McGrew MJ, Robertson CD, Smith J, Haslam C, Barnard P, Radcliffe PA, Mitrophanous KA, Elliot EA, Sang HM (2007): Oviduct- specific expression of two therapeutic proteins in transgenic hens. Proc Natl Acad Sci USA 104:1771- 1776. https://doi.org/10.1073/pnas.0610401104
  9. Miller AD, Rosman GJ (1989): Improved retroviral vectors for gene transfer and expression. Biotechniques 7:980-990.
  10. Mozdziak PE, Petitte JN (2004): Status of transgenic chicken models for developmental biology. Dev Dyn 229:414-421. https://doi.org/10.1002/dvdy.10461
  11. Muramatsu T, Mizutani Y, Ohmori Y, Okumura J (1997): Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo. Biochiem Biophys Res Commun 230: 376-380. https://doi.org/10.1006/bbrc.1996.5882
  12. Paar M, Klein D, Salmons B, Günzburg WH, Renner M, Portsmouth D (2009): Influence of vector design and host cell on the mechanism of recombination and emergence of mutant subpopulations of replicating retroviral vectors. BMC Mol Biol 10:8. https://doi.org/10.1186/1471-2199-10-8
  13. Raju TS, Briggs JB, Borge SM, Jones AJ (2000): Species-specific variation in glycosylation of IgG: evidence for the species-specific sialylation and branch-specific galactosylation and importance for engineering recombinant glycoprotein therapeutics. Glycobiology 10:477-486. https://doi.org/10.1093/glycob/10.5.477
  14. Rapp JC, Harvey AJ, Speksnijder GL, Hu W, Ivarie R (2003): Biologically active human interferon $\alpha$-2b produced in the egg white of transgenic hens. Transgenic Res 12:569-575. https://doi.org/10.1023/A:1025854217349
  15. Rapp JC, Harvey AJ, Speksnijder GL, Hu W, Ivarie R (2003): Biologically active human interferon-2b produced in the egg white of transgenic hens. Transgenic Res 12:569-575. https://doi.org/10.1023/A:1025854217349
  16. Thoraval P, Afanassiefff M, Cosset FL, Lasserre F, Verdier G, Coudert F, Dambrine G (1995): Germline transmission of exogenous genes in chickens using helper-free ecotropic avian leucosis virus-based vectors. Transgenic Res 4:369-377. https://doi.org/10.1007/BF01973755
  17. Zufferey R, Donello JE, Trono D, Hope TJ (1999): Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. J Viol 73:2886-2892. (Received: 28 August 2013/ Accepted: 5 September 2013)