생약학회지 (Korean Journal of Pharmacognosy)

  • 제44권3호
  • /
  • Pages.291-297
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  • 2013
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  • 0253-3073(pISSN)
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  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

LPS로 유도된 RAW264.7 세포주에서 개화정도별 괴화 EtOH 추출물의 항염증 효과 비교연구

A Comparison of Anti-inflammatory Effects of Sophorae Flos EtOH Extracts at the Different Flowering Stages on LPS-induced Inflammation in RAW264.7 cells

  • 고원민 (원광대학교 약학대학 천연물신소재은행) ;
  • 이동성 (원광대학교 한방체액조절연구센터) ;
  • 장미 (원광대학교 약품연구소) ;
  • 김경수 (원광대학교 약학대학 천연물신소재은행) ;
  • 이희숙 (원광대학교 대학원 식품융복합학과) ;
  • 백흠영 (한국인스팜) ;
  • 오현철 (원광대학교 약학대학 천연물신소재은행) ;
  • 김윤철 (원광대학교 약학대학 천연물신소재은행)
  • Ko, Wn-Min (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Lee, Dong-Sung (Hanbang Body-Fluid Research Center, Wonkwang University) ;
  • Jang, Mi (Institute of Pharmaceutical Research and Development,College of Pharmacy, Wonkwang University) ;
  • Kim, Kyoung-Su (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Lee, Hee-Suk (College of human environmental sciences, Department of food industry convergence, Wonkwang University) ;
  • Baek, Hum-Young (Korea INSpharm Research Institute) ;
  • Oh, Hyuncheol (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Kim, Youn-Chul (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
  • 투고 : 2013.08.06
  • 심사 : 2013.09.13
  • 발행 : 2013.09.30
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초록

Sophorae Flos (SF), a composite of flowers and flower-buds of Sophora japonica, has long been used in traditional Korean and Chinese medicines for the treatment of hemostasis and inflammation. In this study, we investigated anti-inflammatory effect of four EtOH extracts at the difference in blooming stages of flowers on LPS-induced inflammation in RAW264.7 cells. We classified the flowers of Sophora japonica with SF-1 (length of flower is shorter than calyx), SF-2 (length of calyx is shorter than flower), SF-3 (full bloom), and SF-4 (not blooming at all). We examined HPLC analysis, whether quercetin and rutin are major component of these Sophorae Flos extracts or not. As a result, SF-1 contained quercetin, but the others did not. In addition, quercetin, SF-1, and SF-4 act on the suppression of pro-inflammatory mediators including inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, nitric oxide (NO), prostaglandin E2 ($PGE_2$) against lipopolysaccharide (LPS)-induced activation in RAW264.7 cells. Of these, SF-1 showed the best anti-inflammatory effect. These results suggest that Sophorae Flos with the highest content of quercetin would be used for the treatment of various inflammation diseases.

키워드

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