Natural Product Sciences

  • 제19권3호
  • /
  • Pages.242-250
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  • 2013
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  • 1226-3907(pISSN)
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  • 2288-9027(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

Isolation and Identification of an Autophagy-inducing Compound from Raphani Semen

  • Gu, Ming-Yao (Natural Medicine Center, Korea Institute of Science and Technology) ;
  • Kwon, Hak Cheol (Natural Medicine Center, Korea Institute of Science and Technology) ;
  • Song, Min Ok (Natural Medicine Center, Korea Institute of Science and Technology) ;
  • Ko, Hyeonseok (Natural Medicine Center, Korea Institute of Science and Technology) ;
  • Cha, Jin-Wook (Natural Medicine Center, Korea Institute of Science and Technology) ;
  • Lee, Won Jong (Gangneung-Wonju National University) ;
  • Yang, Hyun Ok (Natural Medicine Center, Korea Institute of Science and Technology)
  • 투고 : 2013.05.24
  • 심사 : 2013.07.16
  • 발행 : 2013.09.30
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초록

The autophagy-lysosomal pathway is an important protein degradation system, and its dysfunction has been implicated in a number of neurodegenerative diseases, including Parkinson's disease. Raphani Semen, one of the herbs of Yeoldahanso-tang (YH), has neuroprotective effects via the autophagy pathway. The activity-guided method was used to isolate and identify the components of Raphani Semen. In this experiment, the total extract of Raphani Semen was partitioned to n-butanol, methylene chloride, and water fractions. Flow cytometry data showed that only the water fraction showed autophagy-inducing activity in vitro. Compounds 1 and 2 were isolated from this water fraction by preparative HPLC separation. The structures of compounds 1 and 2 were identified as stachyose and raffinose, respectively, by the analysis of various spectral data ($^1H$ NMR, $^{13}C$ NMR, and MS) and comparisons with standard stachyose and raffinose. Of these two compounds, raffinose showed autophagy-inducing activity in PC12 cells through the mTOR pathway.

키워드

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