Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Molecular and Histopathological Evidence of Mycobacteriosis in Paradise Fish Macropodus opercularis Imported into Korea

  • Han, Hyun-Ja (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Jeong-Ho (Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University) ;
  • Jeon, Chan-Hyeok (Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University) ;
  • Kim, Wi-Sik (The Fisheries Science Institute, Chonnam National University) ;
  • Kim, Do-Hyung (Department of Aqualife Medicine, Chonnam National University) ;
  • Jung, Sung-Ju (Department of Aqualife Medicine, Chonnam National University) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, Chonnam National University)
  • Received : 2013.02.05
  • Accepted : 2013.06.17
  • Published : 2013.09.30
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Abstract

We report on mycobacteriosis in an imported tropical ornamental fish Macropodus opercularis commonly known as the paradise fish. Mass mortality occurred in paradise fish imported to Korea from Southeast Asia in 2008. The affected fish did not show any outward clinical signs, but enlargement of the spleen, kidneys, and liver was observed on dissection. Histopathological examination revealed numerous granulomas in the spleen, and acid-fast bacilli were observed in the centers of the granulomas. About 65% of spleen DNA samples were PCR positive using mycobacteria-specific primers targeting the 16S rRNA and hsp65 genes. The nucleotide identities of the 16S rRNA and hsp65 genes with those of Mycobacterium marinum were 99.5% and 99.4%, respectively. Although the bacterium was not cultured, the molecular diagnosis and histopathological findings were consistent with mycobacteriosis in paradise fish.

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References

  1. Adekambi T and Drancourt M. 2004. Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing. Int J Syst Evol Microbiol 54, 2095-2105. http://dx.doi.org/10.1099/ijs.0.63094-0.
  2. Aranaz A, Gibello A, Alvarez J, Mata AI, Rodriguez A, Fallola C, Fernandez-Garayzabal JF and Dominguez L. 2008. Mycobacterium peregrinum infection in farmed European tench (Tinca tinca L.). Vet Microbiol 131, 393-399. http://dx.doi.org/10.1016/j.vetmic.2008.04.017.
  3. Astrofsky KM, Schrenzel MD, Bullis RA, Smolowitz RM and Fox JG. 2000. Diagnosis and management of atypical Mycobacterium spp. infections in established laboratory zebrafish (Brachydanio rerio) facilities. Comp Med 50, 666-672.
  4. Bland CS, Ireland JM, Lozano M, Alvarez ME and Primm TP. 2005. Mycobacterial ecology of the Rio Grande. Appl Environ Microbiol 71, 5719-5727. http://dx.doi.org/10.1128/AEM.71.10.5719-5727.2005.
  5. Chinabut S. 1999. Mycobacteriosis and nocardiosis. In: Fish Diseases and Disorders. Vol. 3. Viral, Bacterial and Fungal Infections. Woo PTK and Bruno DW, eds. CABI Publishing, Wallingford, GB, pp. 319-340.
  6. Corner LA and Trajstman AC. 1988. An evaluation of 1-hexadecylpyridinium chloride as decontaminant in the primary isolation of Mycobacterium bovis from bovine lesions. Vet Microbiol 18, 127-134. http://dx.doi.org/10.1016/0378-1135(88)90058-2.
  7. Decostere A, Hermans K and Haesebrouck F. 2004. Piscine mycobacteriosis: a literature review covering the agent and the disease it causes in fish and humans. Vet Microbiol 99, 159-166. http://dx.doi.org/10.1016/j.vetmic.2003.07.011.
  8. Frevel T, Schafer KL, Totsch M, Bocker W and Dockhorn-Dworniczak B. 1999. PCR based detection of mycobacteria in paraffin wax embedded material routinely processed for morphological examination. Mol Pathol 52, 283-288. http://dx.doi.org/10.1136/mp.52.5.283.
  9. Gauthier DT and Rhodes MW. 2009. Mycobacteriosis in fishes: a review. Vet J 180, 33-47. http://dx.doi.org/10.1016/j.tvjl.2008.05.012.
  10. Gomez S. 2008. Prevalence of microscopic tubercular lesions in freshwater ornamental fish exhibiting clinical signs of non-specific chronic disease. Dis Aquat Org 80, 167-171. http://dx.doi.org/10.3354/dao01939.
  11. Kolk AHJ, Schuitema ARJ, Kuijper S, van Leeuwen J, Hermans PWM, van Embden JDA and Hartskeerl RA. 1992. Detection of Mycobacterium tuberculosis in clinical samples by using polymerase chain reaction and a nonradioactive detection system. J Clin Microbiol 30, 2567-2575.
  12. Kox LFF, van Leeuwen J, Kuijper S, Jansen HM and Kolk AHJ. 1995. PCR assay based on DNA coding for 16S rRNA for detection and identification of mycobacteria in clinical samples. J Clin Microbiol 33, 3225-3233.
  13. Kusunoki S and Ezaki T. 1992. Proposal of Mycobacterium peregrinum sp. nov., nom. rev., and elevation of Mycobacterium chelonae subsp. abscessus (Kubica et al.) to species status: Mycobacterium abscessus comb. nov. Int J Syst Evol Microbiol 42, 240-245. http://dx.doi.org/10.1099/00207713-42-2-240.
  14. Lescenko P, Matlova L, Dvorska L, Bartos M, Vavra O, Navratil S, Novotny L and Pavlik I. 2003. Mycobacterial infection in aquarium fish. Vet Med 48, 71-78.
  15. Levi MH, Bartell J, Gandolfo L, Smole SC, Costa SF, Weiss LM, Johnson LK, Osterhout G and Herbst LH. 2003. Characterization of Mycobacterium montefiorense sp. nov., a novel pathogenic Mycobacterium from moray eels that is related to Mycobacterium triplex. J Clin Microbiol 41, 2147-2152. http://dx.doi.org/10.1128/JCM.41.5.2147-2152.2003.
  16. Man SH and Hodgkiss IJ. 1981. Hong Kong Freshwater Fishes. Urban Council, Wishing Printing Company, Hong Kong, CH.
  17. Marci D, Lo Verde V, Mancuso I, Reale S, Passantino A and Marino F. 2008. Mycobacteriosis in ornamental fish: case reports in Sicily and medical-legal considerations. Vet Res Commun 32, S215-S217. http://dx.doi.org/10.1007/s11259-008-9157-4.
  18. Noordhoek GT, Kaan JA, Mulder S, Wilke H and Kolk AHJ. 1995. Routine application of the polymerase chain reaction for detection Mycobacterium tuberculosis in clinical samples. J Clin Pathol 48, 810-814. http://dx.doi.org/10.1136/jcp.48.9.810.
  19. Pate M, Jencic V, Zolnir-Dovc M and Ocepek M. 2005. Detection of mycobacteria in aquarium fish in Slovenia by culture and molecular methods. Dis Aquat Org 64, 29-35. http://dx.doi.org/10.3354/dao064029.
  20. Rhodes MW, Kator H, Kotob S, van Berkum P, Kaattari I, Vogelbein W, Quinn F, Floyd MM, Butler WR and Ottinger CA. 2003. Mycobacterium shottsii sp. nov., a slowly growing species isolated from Chesapeake Bay striped bass (Morone saxatilis). Int J Syst Evol Microbiol 53, 421-424. http://dx.doi.org/10.1099/ijs.0.02299-0.
  21. Talaat AM, Reimschuessel R and Trucksis M. 1997. Identification of mycobacteria infecting fish to the species level using polymerase chain reaction and restriction enzyme analysis. Vet Microbiol 58, 229-237. http://dx.doi.org/10.1016/S0378-1135(97)00120-X.
  22. Tamura K, Dudley J, Nei M and Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24, 1596-1599. http://dx.doi.org/10.1093/molbev/msm092.
  23. Telenti A, Marchesi F, Balz M, Bally F, Bottger EC and Bodmer T. 1993. Rapid identification of Mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31, 175-178.
  24. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F and Higgins DG. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876-4882. http://dx.doi.org/10.1093/nar/25.24.4876.
  25. Whipps CM, Dougan ST and Kent ML. 2007. Mycobacterium haemophilum infections of zebrafish (Danio rerio) in research facilities. FEMS Microbiol Lett 270, 21-26. http://dx.doi.org/10.1111/j.1574-6968.2007.00671.x.
  26. Whittington RJ and Chong R. 2007. Global trade in ornamental fish from an Australian perspective: the case for revised import risk analysis and management strategies. Prev Vet Med 81, 92-116. http://dx.doi.org/10.1016/j.prevetmed.2007.04.007.

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