Weed & Turfgrass Science

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Occurrence of Pythium Blight Caused by Pythium aphanidermatum on Chewing Fescue

Pythium aphanidermatum에 의한 Chewing Fescue에 잎마름병 발생

  • Chang, Taehyun (School of Ecology & Environmental System, College of Ecology & Environmental Sciences, Kyungpook National University) ;
  • Lee, Yong Se (Division of Life and Environmental Science, Daegu University)
  • 장태현 (경북대학교 생태환경대학 생태환경시스템학부) ;
  • 이용세 (대구대학교 생명환경과학부)
  • Received : 2013.08.26
  • Accepted : 2013.09.09
  • Published : 2013.09.30
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Abstract

Pythium blight occurred by Pythium aphanidermatum on chewing fescue cv. "Jamestowm II" from early June, 2010 and 2011 at the test field in Daegu University in Gyeongbuk Province, Korea. Disease symptoms on the turfgrass were leaf blights dying from the leaf tip and root rot, which appeared patches of brown to dark brown color or gray brown color in the field. The pathogens (40-1 isolate) of Pythium blight was isolated from the diseased leaf and crown tissue and cultured on potato-dextrose agar (PDA) for identification. Lobulate sporangia were inflated, complex structures, and filamentous sporangia were usually indistinguishable from vegetative hyphae. Sequences of ribosomal RNA gene of the fungus were homologous with similarity of 100% to those of P. aphanidermatum isolates in GenBank database. Pathogenicity was also confirmed on the chewing fescue, creeping betgrass and Kentucky bluegrass by Koch's postulates. This is the first report of Pythium blight on chewing fescue caused by P. aphanidermatum in Korea.

Pythium aphanidermatum에 의한 잎마름병(pythium blights)이 chewing fescue "Jamestowm II" 품종에서 2011년 6월 상순에 대구대학교 시험포장에서 발생하였다. 잔디에 발병증상은 잎 끝으로부터 잎마름 증상과 뿌리 썩음증상이 나타나고, 잎색은 진한갈색,회 갈색으로 나타났다. 병원균은 병든 잎과 지제부에서 분리를 하였으며, 동정을 위하여 PDA에서 배양하였다. 균사에는 잎 같은 포자가 복잡한 구조로 부풀어져 있고, 균사로부터 구별되지 않은 실같은 포자낭이 있다. 리보소옴 DNA의 염기서열을 분석한 결과, 유전자은행 DB에 P. aphanidermatum 균주 와의 100% 상동성을 나타낸다는 것을 확인하였다. 병원성은 chewing fescue, creeping bentgrass 및 Kentucky bluegrass에서 코흐의 원칙에 따라 확인하였다. 이것은 chewing fescue 종에서 P. aphanidermatum 에 의한 잎마름병을 국내에서 처음보고 한다.

Keywords

References

  1. Abad, Z.G., Shew, H.D. and Lucas, L.T. 1994. Characterization and pathogenicity of Pythium, species isolated from turfgrass with symp-toms of root and crown rot in North Carolina. Pytopathology 84:913-921. https://doi.org/10.1094/Phyto-84-913
  2. Andrews, E.A. 1943. Seedling blight and root rot of grasses in Minnesota. Phytopathology 33:234-239.
  3. Chen, W. and, Hoy, J.W. 1993. Molecular and morphological comparison of Pythium arrhenomanes and P. graminicola. Mycol. Res. 97:371-1378.
  4. Couch, H.B. 1995. Disease of Turfgrass. 3rd (eds). Krieger Publishing Co., Malabar, FL, USA.
  5. Chang, T. 2011. Difference of susceptibility on bentgrass cultivars to pink snow mold caused by Microdochium nivale. Asian J. Turfgrass Sci. 25(2):177-183
  6. Chang, T. and Lee, Y.S. 2013.Occurrence of brown patch on Kentucky bluegrass caused by Rhizoctonia solani AG-1 IB. Weed Turf. Sci. 2(1):88-94. (In Korean) https://doi.org/10.5660/WTS.2013.2.1.088
  7. Dick, M.W. 1990. Keys to Pythum. University of Reading Press, Reading, UK.
  8. Feng, Y. and Dernoeden, P.H. 1999. Pythum species associated with root dysfunction of creeping bentgrass in Maryland. Plant Dis. 83:516-520. https://doi.org/10.1094/PDIS.1999.83.6.516
  9. Freeman, T.E. 1980. Seedling disease of turf-grasses incited by Pythium. pp. 41-44 in: Advances in turfgrass pathology. Larsen, P.O. and Joyner, B.G. (eds). Harcourt Brace Jovanovich, Duluth, MN, USA.
  10. Hendrix, Jr. F.F., Campbell, W.A. and Mon-crief, J.B. 1970. Pythium species associated with golf turfgrass in the South and Southeast. Plant Dis. Rep. 54: 419-421.
  11. Hodges, C.F. and Coleman, L.W. 1985. Pythium-induced root dysfunction of secondary roots of Agrostis palustris. Plant Dis. 69:336-340.
  12. Hsiang, T., Wu, C., Yang, L. and Liu, L. 1995. Pythium root rot associated with cool-season dieback of turfgrass in Ontario and Quebec. Can. Plant Dis. Surv. 75:191-195.
  13. Kageyama, K., Uchino, H. and Hyakumachi, M. 1998. Characterization of hyphal swelling group of Pythium: DNA polymorphisms and cultural and morphological characteristics. Plant Dis. 82:218-222. https://doi.org/10.1094/PDIS.1998.82.2.218
  14. Kim, J.W. and Park, E.W. 1997. Pythium spp. isolated from turfgrass at golf courses in Korea. Kor. J. Mycol. 25(4):276-290. (In Korean)
  15. Matthews, V.D. 1931. Studies on the genus Pythium. University of North Carolina Press, Chapel Hill, NC, USA.
  16. Matumoto, C., Kageyama, K., Suga, H. and Hyakumachi, M. 2000. Intraspecific DNA polymorphisms of Pythium irregular. Mycol. Res. 104:1333-1341. https://doi.org/10.1017/S0953756200002744
  17. Montheith, J. 1933. A Pythium disease of turf. Phytopathology 23:23-24
  18. Muse, R.R., Schmitthenner, A.F. and Parthka, R.E. 1974. Pythium spp. Associated with foliar blighting of creeping bentgrass. Phytopathology 64:252-253. https://doi.org/10.1094/Phyto-64-252
  19. Saladini, J.L., Schmitthenner, A.F. and Larsen, P.O. 1983. Prevalence of Pythium species as-sociated with cottonyblighted and healthy turfgrass in Ohio. Plant Dis. 67:517-519. https://doi.org/10.1094/PD-67-517
  20. Uzuhashi, S., Tojo, M. and Kakishima, M. 2010. Phylogeny of the genus Pythium and description of new genera. Mycoscience 51(5):337-365 https://doi.org/10.1007/S10267-010-0046-7
  21. Van der Plaats-Niterink, A.J. 1981. Monograph of the genus Pythium. Studies in Mycology No. 21, Centraalbureau Voor Schimmelcul-tures, Baarn, Netherlands. p. 242.
  22. White, T.J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. and, White, T.J. (eds) PCR protocols: a guide to methods and applications. Academic, San Diego, USA. pp. 315-322.

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