생명과학회지 (Journal of Life Science)

  • 제23권9호
  • /
  • Pages.1140-1146
  • /
  • 2013
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

Lipopolysaccharide (LPS)에 의해 유도된 염증에 대한 건조 고등어 추출물의 항염증 효과

Protective Effect of Dried Mackerel Extract on Lipopolysaccharide-induced Inflammation

  • 김광혁 (고신대학교 의과대학 미생물학 교실) ;
  • 최명원 (고신대학교 의과대학 미생물학 교실) ;
  • 최향미 (한국해양대학교 해양환경생명과학부) ;
  • 임선영 (한국해양대학교 해양환경생명과학부)
  • Kim, Kwang-Hyuk (Department Microbiology, Kosin University College of Medicine) ;
  • Choi, Myoung Won (Department Microbiology, Kosin University College of Medicine) ;
  • Choi, Hyang Mi (Division of Marine Environment & Bioscience, Korea Maritime and Ocean University) ;
  • Lim, Sun-Young (Division of Marine Environment & Bioscience, Korea Maritime and Ocean University)
  • 투고 : 2013.08.02
  • 심사 : 2013.09.09
  • 발행 : 2013.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 건조 고등어 추출물 및 분획물들에 의한 nitric oxide 생성에 미치는 영향을 살펴보았고 건조 고등어 85% aq. MeOH 분획물을 중심으로 면역과정의 생물학적 작용과 대사적 변화를 유도하는 IL-6, IFN-${\gamma}$ 및 TNF-${\alpha}$ 같은 pro-inflammatory 사이토카인의 생성을 측정하여 건조 고등어 추출물에 의한 항염증 효과에 대하여 검토하였다. 건조 고등어 추출물과 각 분획물은 control보다 낮은 nitric oxide 생성량을 나타내었으며, 특히 85% aq. MeOH 및 n-hexane 분획물에 의한 저해효과가 높았다. 건조 고등어 분획물은 Con-A 보다는 LPS에 의해 자극된 IL-6, TNF-${\alpha}$ 및 IFN-${\gamma}$의 생성을 감소시키는데 더 효과적이었다. IL-6 및 TNF-${\alpha}$의 생성은 배양시간 6시간 후 건조 고등어 85% aq. MeOH 분획물의 모든 첨가농도(1, 3 및 $10{\mu}g$)에서 감소되었다(p<0.05). IFN-${\gamma}$의 경우, 건조 고등어 85% aq. MeOH 분획물을 LPS와 함께 처리했을 때 특히 72시간 배양 시 IFN-${\gamma}$의 생성량이 농도 의존적으로 감소하였고 Con-A에 의해 자극된 IFN-${\gamma}$의 생성량은 6 및 24시간 배양 후 유의적으로 감소하였다(p<0.05). 이상의 결과로부터 건조 고등어 85% aq. MeOH 분획물은 nitric oxide 생성과 pro-inflammatory 사이토카인(IL-6, TNF-${\alpha}$, and IFN-${\gamma}$)을 감소시켜 염증반응을 예방할 것으로 기대된다.

The effect of dried mackerel extract on the production of nitric oxide (NO) and cytokines, including interleukin-6 (IL-6), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), and interferon-${\gamma}$ (IFN-${\gamma}$), was investigated. All extracts and fractions from dried mackerel significantly reduced NO production induced by lipopolysaccharide (LPS). Among the extracts, acetone+methylene chloride (A+M), n-hexane, and 85% aqueous methanol (MeOH) showed the strongest inhibitory effects. The 85% aqueous MeOH fraction at a concentration of $10{\mu}g$ significantly decreased LPS-induced IL-6 and TNF-${\alpha}$ production after 6 hr of incubation. In the case of LPS-induced IFN-${\gamma}$ production, the 85% aqueous MeOH fraction decreased the production of IFN-${\gamma}$ afer 6, 24, and 72 hr of incubation in a dose-dependent manner. The results show that an 85% aqueous MeOH fraction inhibits the production of NO and proinflammatory cytokines (IL-6, TNF-${\alpha}$, IFN-${\gamma}$), suggesting that this fraction acts as a potent immunomodulator.

키워드

참고문헌

  1. Axtelle, T. and Pribble, J. 2001. IC14, a CD14 specific monoclonal antibody, is a potential treatment for patients with severe sepsis. J Endotoxin Res 7, 310-314. https://doi.org/10.1179/096805101101532783
  2. Bouwens, M., van de Rest, O., Dellsscharft, N., Bromhaar, M. G., de Groot, L. C., Geleijinse, J. M., Muller, M. and Afman, L. A. 2009. Fish-oil supplementation induces antiinflammatory gene expression profiles in human blood mononuclear cells. Am J Clin Nutr 90, 415-424. https://doi.org/10.3945/ajcn.2009.27680
  3. Brown, K. L., Cosseau, C., Gardy, J. L. and Hancock, R. E. 2007. Complexities of targeting innate immunity to treat infection. Trends Immunol 28, 260-266. https://doi.org/10.1016/j.it.2007.04.005
  4. Calder, P. C. 2008. The relationship between the fatty acid composition of immune cells and their function. Prostagglandins Leukot Essent Fatty Acids 79, 101-108. https://doi.org/10.1016/j.plefa.2008.09.016
  5. Caughey, G. E., Mantzioris, E., Gibson, R. A., Cleland, L. G. and James, M. J. 1996. The effect on human tumor necrosis factor and a interleukin 1$\beta$ production of diets enriched in n-3 fatty acid from vegetable oil or fish oil. Am J Clin Nutr 63, 116-122.
  6. Delgado, A. V., McManus, A. T. and Chambers, J. P. 2003. Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin-2, and interleukin-6 by rat leukocyte subpopulation after exposure to substance P. Neuropeptides 37, 355-361. https://doi.org/10.1016/j.npep.2003.09.005
  7. De Smedt-Peyrusse, V., Sargueil, F., Moranis, A., Harizi, H., Mongrand, S. and Laye, S. 2008. Docosahexaenoic acid prevents lipopolysaccharide-induced cytokine production in microglial cells by inhibiting lipopolysaccharide receptor presentation but not its membrane subdomain localization. J Neurochem 105, 296-307. https://doi.org/10.1111/j.1471-4159.2007.05129.x
  8. Feldmann, M., Brennan, F. M. and Maini, R. N. 1996. Role of cytokines in rheumatoid arthritis. Annu Rev Immunol 14, 397-440. https://doi.org/10.1146/annurev.immunol.14.1.397
  9. Green, L. C., Wagner, D. A., Logowski, G. J., Skipper, P. L., Wishnok, J. S. and Tannenbaum, S. R. 1982. Analysis of nitrate, nitrite and [15N] nitrate in biological fluids. Anal Biochem 126, 131-138. https://doi.org/10.1016/0003-2697(82)90118-X
  10. Hwang, S. A., Dasgupta, A. and Actor, J. K. 2004. Cytokine production by non-adherent mouse splenocyte cultures to Echinacea extracts. Clin Chim Acta 343, 161-166. https://doi.org/10.1016/j.cccn.2004.01.011
  11. Hwang, W. I., Baik, N. G., Hwang, Y. K. and Lee, S. D. 1992. Antitumor and immunological effects of tuna extracts. J Korean Soc Food Nutr 21, 353-366.
  12. Jang, J. R., Choi, H. J., Kim, K. K. and Lim, S. Y. 2008. Effect of extracts from dried mackerel on antioxidant activity and inhibition of growth of human cancer cell lines. J Life Sci 18, 680-685. https://doi.org/10.5352/JLS.2008.18.5.680
  13. Kaltschmidt, B., Sparna, T. and Kaltschnidt, D. 1999. Activation of NF-kappa B by reactive oxygen intermediates in the nervous system. Antioxid Redox Signal 1, 129-144. https://doi.org/10.1089/ars.1999.1.2-129
  14. Khair-El-Din, T., Sicher, S. C., Vazquez, M. A., Chung, G. W., Stallworth, K. A., Kitamura, K., Miller, R. T. and Lu, C. Y. 1996. Transcription of the murine iNOS gene is inhibited by docosahexaenoic acid, a major constituent of fetal and neonatal sera as well as fish oils. J Exp Med 183, 1241-1246. https://doi.org/10.1084/jem.183.3.1241
  15. Kim, J., Lee, C. M., Jeong, H. J., Kim, D. W. and Lee, K. Y. 2012. Elevated anti-inflammatory effects of eicosapentaenoic acid based self-aggregated glycol chitosan nanoparticles. J Nanosci Nanotechnol 12, 2672-2678. https://doi.org/10.1166/jnn.2012.5685
  16. Kim, K. H., Choi, M. W., Choi, H. M. and Lim, S. Y. 2013. Effect of tuna extracts on production of nitric oxide and inflammatory cytokines. J Korean Food Sci 45, 385-390. https://doi.org/10.9721/KJFST.2013.45.3.385
  17. Kim, K. H., Kim, S. H. and Park, K. Y. 2001. Effects of kimchi extracts on production of nitric oxide by activated macrophages, transforming growth factor-beta 1 of tumor cells and interleukin-6 in splenocytes. J Food Sci 6, 126-132.
  18. Lazarov, S., Balutsov, M. and Ianev, E. 2000. The role of bacterial endotoxins, receptors and cytokines in the pathogenesis of septic (endotoxin) shock. Vutr Boles 32, 33-40.
  19. Medina, I., Auboug, S. P. and Martin, R. P. 1995. Composition of phospholipids of white muscle of six tuna species. Lipids 30, 1127-1135. https://doi.org/10.1007/BF02536613
  20. Miyake, K. 2004. Innate recognition of lipopolysaccharide by Toll-like receptor 4-MD-2. Trends Microbiol 12, 186-192. https://doi.org/10.1016/j.tim.2004.02.009
  21. Murase, T. and Saito, H. 1996. The docosahexaenoic acid content in the lipid of albacore Thunnusalalunga caught in the two separate localities. Fish Sci 62, 634-638.
  22. Palacios, R. 1982. Concanavalin A triggers T lymphocytes by directly interactingwith their receptors for activation. J Immunol 361, 98-106.
  23. Posadas, I., Terencio, M. C., Guilln, I., Ferrndiz, M. L., Coloma, J., Pay, M. and Alcaraz, M. J. 2000. Co-regulation between cyclo-oxygenase-2 and inducible nitric oxide synthase expression in the time course of murine inflammation. Naunyn-Schmiedebergs Arch Pharmacol 361, 98-106. https://doi.org/10.1007/s002109900150
  24. Ruxton, C. H., Reed, S. C., Simpson, M. J. A. and Millington, K. J. 2004. The health benefits of omega- polyunsaturated fatty acids. J Hum Nutr Diet 17, 449-459. https://doi.org/10.1111/j.1365-277X.2004.00552.x
  25. Scott, M. G. and Hancock, R. E. 2000. Cationic antimicrobial peptides and their multifunctional role in the immune system. Crit Rev Immunol 20, 407-431.
  26. Stokes, K. Y., Cooper D., Tailor, A. and Granger, D. N. 2002. Hypercholesterolemia promotes inflammation and microvascular dysfunction: role of niric oxide and superoxide. Free Radical Biol Med 33, 1026-1036. https://doi.org/10.1016/S0891-5849(02)01015-8
  27. Tizard, I. R. 1986. Immunology, pp. 423-441, 2nd eds., Saunders College Publishing: An introduction inflammation, NY, USA.
  28. Weldon, S. M., Mullen, A. C., Loscher, C. E., Hurley, L. A. and Roche, H. M. 2007. Docosahexaenoic acid induces an anti-inflammatory profile in lipopolysaccharide-stimulated human THP-1 macrophages more effectively than eicosapentaemoic acid. J Nutr Biochem 18, 250-258. https://doi.org/10.1016/j.jnutbio.2006.04.003
  29. Wu, D., Meydani, S. N., Meydani, M., Hayek, M. G., Huth, P. and Nicolosi, R. J. 1996. Immunologic effects of marineand plant-derived n-3 polyunsaturated fatty acids in nonhuman primates. Am J Clin Nutr 63, 273-280.

피인용 문헌

  1. Immunostimulatory and Antioxidant Activities of Steamed Garlic Grown in Different Countries vol.31, pp.2, 2016, https://doi.org/10.13103/JFHS.2016.31.2.126