Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antioxidant and Anti-inflammatory Effects of Bletilla striata Reichenbach fil. Fractions as Cosmetic

백급(Bletilla striata Reichenbach fil.) 분획물의 항산화, 항염증 효과와 화장품소재로서의 연구

  • Yoon, Ji-Hun (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Park, Seong-Geun (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Mi-Ji (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Park, Jin-Young (Skin science R&D Center, Cosmelab Co. Ltd.) ;
  • Seo, Kyo-Seong (Small and Medium Business Administration) ;
  • Woo, Kyung-Chul (Korean Research institute for Skin science, Newlife Cosmetics Ltd.) ;
  • Lee, Chang-Eon (Department of Cosmeceutical Science, Daegu Haany University)
  • 윤지훈 (대구한의대학교 화장품약리학과) ;
  • 박성근 (대구한의대학교 화장품약리학과) ;
  • 이미지 (대구한의대학교 화장품약리학과) ;
  • 박진영 ((주)코스메랩) ;
  • 서교성 (대구경북지방중소기업청) ;
  • 우경철 ((주)신생활피부과학연구소) ;
  • 이창언 (대구한의대학교 화장품약리학과)
  • Received : 2013.03.30
  • Accepted : 2013.09.10
  • Published : 2013.09.30
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Abstract

The antioxidant and anti-inflammatory effects of active ingredients in cosmetics are very important. The effects are closely related to the prevention of skin aging. Among medicinal plants, Bletilla striata Reichenbach fil. has well-known pharmacological activity. Extracted samples were prepared using sequential fractionation of ethyl acetate, butanol, and water. The antioxidant effect of the fractions was confirmed by DPPH and ABTS+ radical scavenging. Among the various fractions of B. striata Reichenbach fil., ethyl acetate was associated with a reduction in nitric oxide production, which was induced by LPS (lipopolysaccharide) treatment in a dose-dependent manner. In addition, the production of iNOS (inducible nitric oxide synthase) and COX-2 (cyclooxygenase-2), which are upstream regulators of nitric oxide production, was also inhibited. Thus, the ethyl acetate fraction of B. striata Reichenbach fil. appears to be a potential active ingredient for use in cosmetics.

항산화, 항염증 효과는 화장품 소재에서 중요한 요소이다. 항산화, 항염증은 피부노화 예방과 밀접한 관련이 있다. 백급은 이전부터 약리활성이 뛰어난 것으로 알려져 왔다. 실험재료는 백급의 에틸아세테이트, 부탄올, 물분획물을 사용하였다. 항산화 효과는 DPPH, ABTS+ radical scavenging 실험으로 확인하였다. 다양한 분획물중, 백급의 에틸아세테이트 분획물이 LPS에 유도된 nitric oxide의 생성을 농도 의존적으로 감소시키는 것을 증명하였다. 게다가, nitric oxide 생성의 상위 기작인 iNOS, COX-2 단백질 발현도 저해하였다. 우리는 백급 분획물의 항산화, 항염증 효과가 항 노화 및 피부염증 개선 화장품 소재로서의 가능성을 기대한다.

Keywords

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