한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제36권3호
  • /
  • Pages.193-201
  • /
  • 2013
  • /
  • 3022-7372(pISSN)
  • /
  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지
DOI QR코드

DOI QR Code

돼지고기 제품 내 닭고기 검출을 위한 TaqMan® real-time PCR의 적용

Application of the TaqMan® real-time PCR assay for the detection of chicken (Gallus gallus) meat in pork products

  • 고바라다 (광주광역시보건환경연구원) ;
  • 김지연 (광주광역시보건환경연구원) ;
  • 나호명 (광주광역시보건환경연구원) ;
  • 박성도 (광주광역시보건환경연구원) ;
  • 김용환 (광주광역시보건환경연구원)
  • Koh, Ba-Ra-Da (Health & Environment Research Institute of Gwangju) ;
  • Kim, Ji-Yeon (Health & Environment Research Institute of Gwangju) ;
  • Na, Ho-Myung (Health & Environment Research Institute of Gwangju) ;
  • Park, Seong-Do (Health & Environment Research Institute of Gwangju) ;
  • Kim, Yong-Hwan (Health & Environment Research Institute of Gwangju)
  • 투고 : 2013.08.05
  • 심사 : 2013.09.03
  • 발행 : 2013.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Many consumers are increasingly concerned about the meat they eat, and accurate labelling is important due to public health, economic and legal concerns. Meat species adulteration is a common problem in the retail markets. In this study, a TaqMan$^{(R)}$ quantitative real-time polymerase chain reaction (PCR) assay was applied for its ability to quantify chicken meat, which was not indicated on the label, in 79 commercial pork products (ham, sausages, bacon and ground meat) producted by 10 different manufacturers. The amplification efficiency was 82.05% and the square regression coefficient ($R^2$) was 0.995. PCR results showed that 38.6% of ham samples, 50.0% of sausages samples, and 50.0% of ground meat samples were contaminated with chicken residuals, while the bacon samples were not contaminated with chicken residuals. Only twelve pork products of one of the manufacturers were in accordance with indicated in their labels. The PCR assay reported in this work could be particularly useful in inspection programs to verify the food labelling of commercial processed meats and to gain consumers' trust.

키워드

참고문헌

  1. Ballin NZ. 2010. Authentication of meat and meat products. Meat Science 86: 577-587. https://doi.org/10.1016/j.meatsci.2010.06.001
  2. Ballin NZ, Vogensen FK, Karlsson AH. 2009. Species determination - Can we detect and quantify meat adulteration? Meat Science 83: 165-174. https://doi.org/10.1016/j.meatsci.2009.06.003
  3. Bottero MT, Dalmasso A. 2011. Animal species identification in food products: evolution of biomolecular methods. Vet J 190: 34-38. https://doi.org/10.1016/j.tvjl.2010.09.024
  4. Buntjer JB, Lamine A, Haagsma N, Lenstra JA. 1999. Species identification by oligonucleotide hybridisation: the influence of processing of meat products. J Sci Food Agric 79: 53-57. https://doi.org/10.1002/(SICI)1097-0010(199901)79:1<53::AID-JSFA171>3.0.CO;2-E
  5. Camma C, Domenico MD, Monaco F. 2012. Development and validation of fast Real-Time PCR assays for species identification in raw and cooked meat mixtures. Food Control 23: 400-404. https://doi.org/10.1016/j.foodcont.2011.08.007
  6. Di Pinto A, Forte VT, Conversano MC, Tantillo GM. 2005. Duplex polymerase chain reaction for detection of pork meat in horse meat fresh sausages from Italian retail sources. Food Control 16: 391-394. https://doi.org/10.1016/j.foodcont.2004.04.004
  7. Dooley JJ, Paine KE, Garrett SD, Brown HM. 2004. Detection of meat species using TaqMan real-time PCR assays. Meat Science 68: 431-438. https://doi.org/10.1016/j.meatsci.2004.04.010
  8. Fajardo V, Gonzalez I, Rojas M, Garcia T, Martin R. 2010. A review of current PCR-based methodologies for the authentication of meats from game animal species. Trends in Food Science & Technology 21: 408-421. https://doi.org/10.1016/j.tifs.2010.06.002
  9. Jonker KM, Tilburg JJ, Hagelea GH, de Boer E. 2008. Species identification in meat products using real-time PCR. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 25: 527-533. https://doi.org/10.1080/02652030701584041
  10. Ghovvati S, Nassiri MR, Mirhoseini SZ, Moussavi AH, Javadmanesh A. 2009. Fraud identification in industrial meat products by multiplex PCR assay. Food Control 20: 696-699. https://doi.org/10.1016/j.foodcont.2008.09.002
  11. Koh BRD, Kim JY, Na HM, Park SD, Kim YH. 2011. Development of species-specific multiplex PCR assays of mitochondrial 12S rRNA and 16S rRNA for the identification of animal species. Korean J Vet Serv 34: 297-301. https://doi.org/10.7853/kjvs.2011.34.4.297
  12. Koh BRD, Kim JY, Na HM, Park SD, Kim YH. 2012. Development of $TaqMan^{(R)}$ probe-based real-time PCR for rapid identification of beef, pork and poultry meat. Korean J Vet Serv 35: 215-222. https://doi.org/10.7853/kjvs.2012.35.3.215
  13. Laube I, Spiegelberg A, Butschke A, Zagon J, Schauzu M, Kroh L, Broll H. 2003. Methods for the detection of beef and pork in foods using real-time polymerase chain reaction. Food Sci Technol Int 38: 111-118. https://doi.org/10.1046/j.1365-2621.2003.00651.x
  14. Laube I, Zagon J, Broll H. 2007a. Quantitative determination of commercially relevant species in foods by real-time PCR. Food Sci Technol Int 42: 336-341. https://doi.org/10.1111/j.1365-2621.2006.01249.x
  15. Laube I, Zagon J, Spiegelberg A, Butschke A, Kroh LW, Broll H. 2007b. Development and design of a 'ready-to-use' reaction plate for a PCR-based simultaneous detection of animal species used in foods. Food Sci Technol Int 42: 9-17. https://doi.org/10.1111/j.1365-2621.2006.01154.x
  16. Lopez-Andreo M, Lugo L, Garrido-Pertierra A, Prieto MI, Puyet A. 2005. Identification and quantitation of species in complex DNA mixtures by real-time polymerase chain reaction. Anal Biochem 339: 73-82. https://doi.org/10.1016/j.ab.2004.11.045
  17. Lopparelli RM, Cardazzo B, Balzan S, Giaccone V, Novelli E. 2007. Real-time TaqMan polymerase chain reaction detection and quantification of cow DNA in pure water buffalo mozzarella cheese: method validation and its application on commercial samples. J Agric Food Chem 55: 3429-3434. https://doi.org/10.1021/jf0637271
  18. Meyer R, Hofelein C, Luthy J, Candrian U. 1995. Polymerase chain reaction-restriction fragment length polymorphism analysis: a simple method for species identification in food. J AOAC Int 78: 1542-1551.
  19. Montiel-Sosa JF, Ruiz-Pesini E, Montoya J, Roncales P, Lopez-Perez MJ, Perez-Martos A. 2000. Direct and highly species-specific detection of pork meat and fat in meat products by PCR amplification of mitochondrial DNA. J Agric Food Chem 48: 2829-2832. https://doi.org/10.1021/jf9907438
  20. Prado M, Fumiere O, Boix A, Marien A, Berben G, von Holst C. 2009. Novel approach for interlaboratory transfer of real-time PCR methods: detecting bovine meat and bone meal in feed. Anal Bioanal Chem 394: 1423-1431. https://doi.org/10.1007/s00216-009-2796-7
  21. Rojas M, Gonzalez I, Pavon MA, Pegels N, Hernandez PE, Garcia T, Martin R. 2011. Application of a real-time PCR assay for the detection of ostrich (Struthio camelus) mislabelling in meat products from the retail market. Food Control 22: 523-531. https://doi.org/10.1016/j.foodcont.2010.09.039
  22. Tanabe S, Hase M, Yano T, Sato M, Fujimura T, Akiyama H. 2007. A real-time quantitative PCR detection method for pork, chicken, beef, mutton, and horseflesh in foods. Biosci Biotechnol Biochem 71: 3131-3135. https://doi.org/10.1271/bbb.70683