한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제28권3호
  • /
  • Pages.272-278
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  • 2013
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
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농산물 접촉 표면 재질에 따른 Escherichia coli O157:H7의 생존 및 상추로의 교차오염도 조사

Survival and Cross-contamination of Escherichia coli O157:H7 on Various Agricultural Product-Contact Surfaces

  • 김세리 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 최송이 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 서민경 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김원일 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 정덕화 (경상대학교 응용생명과학부) ;
  • 류경열 (농촌진흥청 연구정책국 연구성과관리과) ;
  • 윤종철 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김병석 (농촌진흥청 국립농업과학원 기획조정과)
  • Kim, Se-Ri (Microbial Safety Team Department of Crop-Life Safety, NAAS, RDA) ;
  • Choi, Song-Yi (Microbial Safety Team Department of Crop-Life Safety, NAAS, RDA) ;
  • Seo, Min-Kyoung (Microbial Safety Team Department of Crop-Life Safety, NAAS, RDA) ;
  • Kim, Won-Il (Microbial Safety Team Department of Crop-Life Safety, NAAS, RDA) ;
  • Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University) ;
  • Ryu, Kyoung Yul (R&D Evaluation Division, Research Policy Bureau, RDA) ;
  • Yun, Jong-Chul (Microbial Safety Team Department of Crop-Life Safety, NAAS, RDA) ;
  • Kim, Byung-Seok (Planning & Coordination Division, NAAS, RDA)
  • 투고 : 2013.07.01
  • 심사 : 2013.09.06
  • 발행 : 2013.09.30
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초록

본 연구는 polyvinyl chroride (PVC)와 stainless steel 표면에서 E. coli O157:H7의 부착, 바이오필름형성, 온 습도에 따른 생존율을 조사하고, E. coli O157:H7이 오염된 작업대 표면에서 상추로의 교차오염도 조사를 통하여 E. coli O157:H7에 오염된 작업대가 상추의 미생물학적 안전성에 미치는 영향을 평가하고자 수행하였다. 작업대 표면 재질인 PVC와 stainless steel에 E. coli O157:H7 배양액을 1시간 노출시켰을 때, PVC의 경우, stainless steel 보다 10배 정도 부착력이 높았으나, 6시간째는 두 재질간의 차이가 없었다. 또한, E. coli O157:H7의 바이오필름 형성은 TSB > 10% 상추추출액 > 1% 상추추출액 > phosphate buffer 순이었으며, 재질별로는 PVC에서 바이오필름형성능이 높은 것으로 나타났다. 온도 $20^{\circ}C$, $30^{\circ}C$, 습도 43%, 69%, 100%에서 각각 노출 시켰을 때, E. coli O157:H7은 온도 $30^{\circ}C$, 습도 43%, 69% 조건에서 12시간 내에 약 5.0 log CFU/coupon이 감소한데 반해, 상대습도 100%에서는 농도의 큰 변화 없이 5일 이상 생존이 지속되었다. 또한, 유기물이 작업대 표면에 존재 시, E. coli O157:H7의 감소 속도가 느렸다. E. coli O157:H7에 오염된 작업대에 상추를 접촉시키고 상추의 오염도를 조사한 결과, 상추 표면에 수분이 존재할 때 오염된 작업대 표면에서 E. coli O157:H7의 이동수준이 상추에 수분이 없을 때 보다 10배 이상 증가하는 것으로 나타났다. 따라서 E. coli O157:H7에 오염된 작업대는 상추의 안전성에 직접적인 영향을 미칠 수 있어 작업 후에 세척, 소독을 통하여 위생적으로 관리하는 것이 필요하다.

To evaluate the effect of surface contaminated with Escherichia coli O157:H7 (E. coli O157:H7) on the microbiological safety of lettuce, this study was conducted to investigate the attachment, biofilm producing, survival, and cross-contamination of E. coli O157:H7 on stainless steel and polyvinyl chloride (PVC). The attachment rate of E. coli O157:H7 on PVC was 10 times higher than that on stainless steel after exposure 1 h in cell suspension. However, there was not a difference between two types of surface after exposure for 6 h and 24h. The biofilm producing of E. coli O157:H7 was TSB > 10% lettuce extracts > 1% lettuce extracts > phosphate buffer. When two kinds of materials were stored at various conditions ($20^{\circ}C$ and $30^{\circ}C$, relative humidity (RH) 43%, 69%, and 100%), the numbers of E. coli O157:H7 at $30^{\circ}C$, RH 43% or RH 69% were reduced by 5.0 log CFU/coupon within 12 h regardless of material type. Conversely, the survival of E. coli O157:H7 at RH 100% was lasted more than 5 days. In addition, the reduction rate of E. coli O157:H7 was decreased in the presence of organic matter. The transfer efficiency of E. coli O157:H7 from the contaminated surface to lettuce was dependent upon the water amount of the surface of lettuce. Especially, the transfer rate of E. coli O157:H7 was increased by 10 times in the presence of water on the lettuce surface. From this study, the retention of E. coli O157:H7 on produce contact surfaces increase the risk cross-contamination of this pathogen to produce. Thus, it is important that the surface in post harvest facility is properly washed and sanitized after working for prevention of cross-contamination from surface.

키워드

참고문헌

  1. KIM, G.H. and Bang, H.Y.: A survey on consumption pattern of minimally fruits and vegetables. Korean J. Food Culture, 13, 267-274 (1998).
  2. Choi, J.W., Park, S.Y., Yeon, J.H., Lee, M.J., Chung, D.H., Lee, K.H., Kim, M.G., Lee, D.H., Kim, K.S. and Ha, S.D.: Microbial contamination levels of fresh vegetables distributed in markets. Korean J. Fd. Hyg. Safety, 20, 43-47 (2005).
  3. FDA: Guidance for Industry: Guide to Minimize Microbial Food Safety Hazards of Leafy Greens; Draft Guidance. Available from: http://www.fda.gov/Food/GuidanceComplianceRegulatoryInformation/ GuidanceDocuments/ProduceandPlanProducts/ ucm174200.html.
  4. CDC: Update on Multi-State Outbreak of E. coli O157:H7 Infections From Fresh Spinach, October 6, 2006. Available from: http://www.cdc.gov/ecoli/2006/september/updates/100-606.html.
  5. KFDA: Analysis on the trend of food-poising over the last 5 years. Av ailable from: http://www.foodnara.go.kr/portal/site/ kfdaportal/template. Accessed Aug. 25, (2010)
  6. Watanabe, Y., Ozasa, K., Mermin, J.H., Griffin, P.M., Masuda, K., Imashuku, S. and Sawada, T.: Factory outbreak of Escherichia coli O157:H7 infection in Japan. Emerg. Infect. Dis., 5, 424-428 (1999). https://doi.org/10.3201/eid0503.990313
  7. Kim S.R., Lee, J.Y., Lee, S.H., Kim, W.I., Park, K.H., Yun, H.J., Kim, B.S., Chung, D.H., Yun, J.C. and Ryu, K.Y.: Evaluation of microbiological safety of lettuce and cultivation area. Korean J. Fd. Hyg. Safety, 26, 289-295 (2011).
  8. Chai, L.C., Lee, H.Y., Ghazali, F.M., Bakar, F.A., Malakar, P.K., Nishibuchi, M. Nishibuchi, Nakaguchi, Y. and Radu. S.: Simulation of cross-contamination and decontamination of Campylobacter jejuni during handling of contaminated raw vegetables in a domestic kitchen. J. Food Prot., 71, 2448-2452 (2008).
  9. Johnston, L.M., Jaykus, L.A., Moll, D., Martinez, M.C., Anciso, J., Mora, B. and Moe, C.L.:. A field study of the microbiological quality of fresh produce. J. Food Prot., 68, 1840-1847 (2005)
  10. Ailes, E.C., Leon, J.S. Jaykus, L.A., Johnston, L.M., Clayton, H.A., Blanding, S., Kleinbaum, D.G., Backer, L.C. and Moe, C.L.: Microbial concentrations on fresh produce are affected by postharvest processing, importation, and season. J. Food Prot., 71, 2389-2397 (2008)
  11. Bae, Y.M., Heu, S. and Lee, S.Y.: Inhibitiory effect of dryheat treatment and chemical sanitizers against foodborne pathogens contaminated on the surfaces of meterials. J. Korean Soc Food SCi. Nutr., 38, 1265-1270 (2009). https://doi.org/10.3746/jkfn.2009.38.9.1265
  12. Ryu, J.H., Kim, H. and Beuchat, L.R.: Attachment and biofilm formation by Escherichia coli O157:H7 on stainless steel as influence by exopolysaccharide production, nutrient availability, and temperature. J. Food Prot., 67, 2123-2131 (2004).
  13. Park, E.J., Alexander, E., Taylor, G.A., Costa, R. and Kang, D.H.: The decontaminative effects of acidic electrolyzed water for Escherichia coli O157:H7, Salmonella typhimurium, and Listeria monocytogenes on green onions and tomatoes with different organic demands. Food Microbiol., 26, 386-390 (2009). https://doi.org/10.1016/j.fm.2008.10.013
  14. de Rezende, E.E., Anriany, Y., Carr, L.E., Joseph, S.W. and Weiner, R.M.: Capsular polysaccharide surrounds smoothe and rugose types of Salmonella enterica serovar Typhimurium DT104. Appl. Environ. Microbiol., 71, 7345-7351 (2005). https://doi.org/10.1128/AEM.71.11.7345-7351.2005
  15. Paul, J.H., and Jeffrey, W.H.: Evidience for separate adhesion mechanisms for hydrophilic and hydrophobic surfaces in Vibrio proteolytica. Appl. Environ. Microbiol., 50, 431- 437 (1985).
  16. Sinde, E. and Carballo, J.: Attachment of Salmonella spp. and Listeria monocytogenes to stainless steel, rubber and polytetrafluor-ethylene : the influence of free energy and the effect of commercial sanitizers. Food Microbiol., 17, 439- 447 (2000). https://doi.org/10.1006/fmic.2000.0339
  17. Silagyi, K., Kim S.H., Lo, Y.M, and Wei, C.: Production of biofilm and quorum sensing by Escherichia coli O157:H7 and its transfer from contact surface to meat, poultry, readyto- eat deli, and produce products. Food Microbiol., 26, 514- 519 (2009). https://doi.org/10.1016/j.fm.2009.03.004
  18. Bae, Y.M., Baek, S.Y. and Lee, S.Y.: Resistance of pathogenic bacteria on the surface of stainless steel depending on attachment form and efficacy of chemical sanitizers. Int. J. Food Microbiol., 153, 465-473 (2012) https://doi.org/10.1016/j.ijfoodmicro.2011.12.017
  19. Else, T.A., Pantle, C.R. and Amy, P.S.: Boundaries for biofilm formation : humidity and temperature. Appl. Environ. Microbiol., 69, 5006-5010 (2003) https://doi.org/10.1128/AEM.69.8.5006-5010.2003
  20. Takahashi, H., Kuramoto, S., Miya, S. and Kimura, B.: Desiccation survival of Listeria monocytogenes and other potential foodborne pathogens on stainless steel surface is affected by different food soils. Food Control, 22, 633-637 (2011). https://doi.org/10.1016/j.foodcont.2010.09.003
  21. Kusumaningrum, H.D., Riboldi, G., Hazeleger, W.C. and Beumer, R.R.: Survival of foodborne pathogens on stainless steel surfaces and cross-contamination to foods. Int. J. Food Microbiol., 85, 227-236 (2003). https://doi.org/10.1016/S0168-1605(02)00540-8
  22. Kusumaningrum, H.D., van Asselt E.D., Beumer, R.R., and Zwietering, M.H.: A quantitative analysis of cross-contamination of Salmonella and Campylobacter spp. via Domestic kitchen surfaces. J. Food Prot., 67, 1892-1903 (2004).