Journal of Applied Biological Chemistry

  • 제56권3호
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  • Pages.137-139
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  • 2013
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  • 1976-0442(pISSN)
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  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
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On-line Screening HPLC-ABTS를 이용한 강황으로부터 Curcuminoids의 생물활성 분석

Bioactivity Analysis of Curcuminoids from Turmeric using On-line Screening HPLC-ABTS

  • Choi, Sun Do (Department of Chemical Engineering, Kangwon National University Samcheok Campus)
  • 투고 : 2013.04.16
  • 심사 : 2013.04.25
  • 발행 : 2013.09.30
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초록

강황(Curcuma longa)으로부터 bisdemethoxycurcumin (BDMC), demethoxycurcumin (DMC) 및 curcumin의 생물 활성을 offline-ABTS 측정법과 on-line screening high-performance liquid chromatography (HPLC)-ABTS 측정법을 적용한 빠른 스크리닝을 통해 정량 및 성분 분리를 하였다. 이때, off-line-ABTS와 on-line screening HPLC-ABTS 비교는 미미한 오차를 보여주었다.

Free radical scavengers in the bisdemethoxycurcumin (BDMC), demethoxycurcumin (DMC) and curcumin of turmeric (Curcuma longa) were screened, identified, quantified and isolation using coupled off-line-2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and on-line screening high-performance liquid chromatography (HPLC)-ABTS assay. There was a very small margin of error between the off-line-ABTS method and the on-line screening HPLC-ABTS method.

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참고문헌

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