Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Bioactivity Analysis of Curcuminoids from Turmeric using On-line Screening HPLC-ABTS

On-line Screening HPLC-ABTS를 이용한 강황으로부터 Curcuminoids의 생물활성 분석

  • Choi, Sun Do (Department of Chemical Engineering, Kangwon National University Samcheok Campus)
  • Received : 2013.04.16
  • Accepted : 2013.04.25
  • Published : 2013.09.30
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Abstract

Free radical scavengers in the bisdemethoxycurcumin (BDMC), demethoxycurcumin (DMC) and curcumin of turmeric (Curcuma longa) were screened, identified, quantified and isolation using coupled off-line-2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and on-line screening high-performance liquid chromatography (HPLC)-ABTS assay. There was a very small margin of error between the off-line-ABTS method and the on-line screening HPLC-ABTS method.

강황(Curcuma longa)으로부터 bisdemethoxycurcumin (BDMC), demethoxycurcumin (DMC) 및 curcumin의 생물 활성을 offline-ABTS 측정법과 on-line screening high-performance liquid chromatography (HPLC)-ABTS 측정법을 적용한 빠른 스크리닝을 통해 정량 및 성분 분리를 하였다. 이때, off-line-ABTS와 on-line screening HPLC-ABTS 비교는 미미한 오차를 보여주었다.

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References

  1. Choi HY (2009) Isolation and Identification of Antimicrobial Compound from Ulgeum (Curcuma longa L.) J Korean Soc Food Sci Nutr 38, 1202-9. https://doi.org/10.3746/jkfn.2009.38.9.1202
  2. Inoue K, Kitade M, Hino T, and Oka H (2011) Screening assay of angiotensin-converting enzyme inhibitory activity from complex natural colourants and foods using high-throughput LC-MS/MS. Food Chemistry 126, 1909-15. https://doi.org/10.1016/j.foodchem.2010.12.019
  3. Jiang JL, Jin XL, Su HZX, Qiao B, and Yuan YJ (2012) Identification of antitumor constituents in curcuminoids from Curcuma longa L. based on the composition-activity relationship. J Pharm Biomed Anal 70, 664-70. https://doi.org/10.1016/j.jpba.2012.05.011
  4. Lee KJ, Liang C, Yang HJ, and Ma JY (2012) Rapid Identification of Homoorientin from Phyllostachys bambusoides Leaves by HPLC Online ABTS+ Screening Method. Yakhak Hoeji 56, 217-21.
  5. Lee KJ, Ma JY, Kim YS, Kim DS, and Jin YZ (2012) Solid Phase Extraction (SPE) of Curcuminoids from Turmeric by Optimization Analytical Condition. J of the Korea Academia Industrial cooperation Society 13, 4927-35. https://doi.org/10.5762/KAIS.2012.13.10.4927
  6. Lee KJ, Kim YS, and Ma JY (2013) Separation and Identification of Curcuminoids from Asian Turmeric (Curcuma long L.) Using RP-HPLC and LC-MS. Asian Journal of Chemistry 25, 909-12.
  7. Lee KJ, Choi SD, and Ma JY (2013) Phytochemical Analysis of Curcumin from Turmeric by RP-HPLC. Asian Journal of Chemistry 25, 995-8.
  8. Singh G, Kapoor IPS, Singh P, Heluani CS, Lampasona MP, and Catalan CAN (2010) Comparative study of chemical composition and antioxidant activity of fresh and dry rhizomes of turmeric (Curcuma longa Linn.). Food Chem Toxicol 48, 1026-31. https://doi.org/10.1016/j.fct.2010.01.015
  9. Zhao XC, Zhang L, Yu HX, Sun Z, Lin XF, Tan C et al. (2011) Curcumin protects mouse neuroblastoma Neuro-2A cells against hydrogenperoxide-induced oxidative stress. Food Chemistry 129, 387-94. https://doi.org/10.1016/j.foodchem.2011.04.089
  10. Zhang JS, Guan J, Yang FQ, Liu HG, Cheng XJ, and Li SP (2008) Qualitative and quantitative analysis of four species of Curcuma rhizomes using twice development thin layer chromatography. J Pharm & Bio Anal 48, 1024-8. https://doi.org/10.1016/j.jpba.2008.07.006