International Journal of Oral Biology

  • 제38권3호
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  • Pages.127-134
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  • 2013
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
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Xylitol Down-Regulates $1{\alpha},25$-Dihydroxy Vitamin D3-induced Osteoclastogenesis via in Part the Inhibition of RANKL Expression in Osteoblasts

  • 투고 : 2013.07.17
  • 심사 : 2013.08.10
  • 발행 : 2013.09.30
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초록

Xylitol is a sugar alcohol with a variety of functions including bactericidal and anticariogenic effects. However, the cellular mechanisms underlying the role of xylitol in bone metabolism are not yet clarified. In our present study, we exploited the physiological role of xylitol on osteoclast differentiation in a co-culture system of osteoblastic and RAW 264.7 cells. Xylitol treatment of these co-cultures reduced the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells induced by 10 nM $1{\alpha},25(OH)_2D_3$ in a dose-dependent manner. A cell viability test revealed no marked cellular damage by up to 100 mM of xylitol. Exposure of osteoblastic cells to xylitol decreased RANKL, but not OPG, mRNA expression in the presence of $10^{-8}M$ $1{\alpha},25(OH)_2D_3$ in a dose-dependent manner. Furthermore, bone resorption activity, assessed on bone slices in the coculture system, was found to be dramatically decreased with increasing xylitol concentrations. RANKL and OPG proteins were assayed by ELISA and the soluble RANKL (sRANKL) concentration was decreased with an increased xylitol concentration. In contrast, OPG was unaltered by any xylitol concentration in this assay. These results indicate that xylitol inhibits $1{\alpha},25(OH)_2D_3$-induced osteoclastogenesis by reducing the sRANKL/OPG expression ratio in osteoblastic cells.

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참고문헌

  1. Kitchens DH. Xylitol in the prevention of oral diseases. Spec Care Dentist. 2005;25(3):140-4. https://doi.org/10.1111/j.1754-4505.2005.tb01424.x
  2. Wunder D, Bowen WH. Action of agents on glucosyltransferases from Streptococcus mutans in solution and adsorbed to experimental pellicle. Arch Oral Biol. 1999;44(3):203-14. https://doi.org/10.1016/S0003-9969(98)00129-0
  3. Svanberg M, Hietala EL, Knuuttila M. The effect of dietary xylitol on dentin formation in ovariectomized rats. Acta Odontol Scand. 1994;52(2):82-5. https://doi.org/10.3109/00016359409029059
  4. Svanberg M, Knuuttila M. The effect of dietary xylitol on recalcifying and newly formed cortical long bone in rats. Calcif Tissue Int. 1993;53(2):135-8. https://doi.org/10.1007/BF01321892
  5. Svanberg M, Knuuttila M. Dietary xylitol prevents ovariectomy induced changes of bone inorganic fraction in rats. Bone Miner. 1994;26(1):81-8. https://doi.org/10.1016/S0169-6009(08)80164-X
  6. Svanberg M, Knuuttila M. Dietary xylitol retards bone resorption in rats. Miner Electrolyte Metab. 1994;20(3):153-7.
  7. Svanberg M, Mattila P, Knuuttila M. Dietary xylitol retards the ovariectomy-induced increase of bone turnover in rats. Calcif Tissue Int. 1997;60(5):462-6. https://doi.org/10.1007/s002239900263
  8. Burgess TL, Qian Y, Kaufman S, Ring BD, Van G, Capparelli C, et al. The ligand for osteoprotegerin (OPGL) directly activates mature osteoclasts. J Cell Biol. 1999;145(3):527-38. https://doi.org/10.1083/jcb.145.3.527
  9. Fuller K, Wong B, Fox S, Choi Y, Chambers TJ. TRANCE is necessary and sufficient for osteoblast-mediated activation of bone resorption in osteoclasts. J Exp Med. 1998;188(5):997-1001. https://doi.org/10.1084/jem.188.5.997
  10. Kong YY, Yoshida H, Sarosi I, Tan HL, Timms E, Capparelli C, et al. OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis. Nature. 1999;397(6717):315-23. https://doi.org/10.1038/16852
  11. Lacey DL, Timms E, Tan HL, Kelley MJ, Dunstan CR, Burgess T, et al. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell. 1998;93(2):165-76. https://doi.org/10.1016/S0092-8674(00)81569-X
  12. Jeong HJ, Tian Y, Kim BH, Nam MY, Lee HA, Yoo YJ, et al. Hypertonicity Down-regulates the $1{\alpha}$,25(OH)2 Vitamin D3-induced Osteoclastogenesis Via the Modulation of RANKL Expression in Osteoblast. Int J Oral Biol. 2005;30(1):23-30.
  13. Eriksen EF. Cellular mechanisms of bone remodeling. Rev Endocr Metab Disord. 2010;11(4):219-27. https://doi.org/10.1007/s11154-010-9153-1
  14. Aubin JE. Advances in the osteoblast lineage. Biochem Cell Biol. 1998;76(6):899-910. https://doi.org/10.1139/o99-005
  15. Kim MJ, Jun YJ, Yu HI, Yang SY, Oh YM, Kim SH, et al. Altered Expression of RANKL/OPG after Alendronate Administration in the Developing Teeth of Postnatal Rats. Int J Oral Biol. 2011;36(1):37-42.
  16. Suda T, Udagawa N, Nakamura I, Miyaura C, Takahashi N. Modulation of osteoclast differentiation by local factors. Bone. 1995;17(2 Suppl):87S-91S. https://doi.org/10.1016/8756-3282(95)00185-G
  17. Takahashi N, Udagawa N, Suda T. A new member of tumor necrosis factor ligand family, ODF/OPGL/TRANCE/RANKL, regulates osteoclast differentiation and function. Biochem Biophys Res Commun. 1999;256(3):449-55. https://doi.org/10.1006/bbrc.1999.0252
  18. Tsukii K, Shima N, Mochizuki S, Yamaguchi K, Kinosaki M, Yano K, et al. Osteoclast differentiation factor mediates an essential signal for bone resorption induced by 1 alpha, 25-dihydroxyvitamin D3, prostaglandin E2, or parathyroid hormone in the microenvironment of bone. Biochem Biophys Res Commun. 1998;246(2):337-41. https://doi.org/10.1006/bbrc.1998.8610
  19. Jimi E, Akiyama S, Tsurukai T, Okahashi N, Kobayashi K, Udagawa N, et al. Osteoclast differentiation factor acts as a multifunctional regulator in murine osteoclast differentiation and function. J Immunol. 1999;163(1):434-42.
  20. Wong BR, Rho J, Arron J, Robinson E, Orlinick J, Chao M, et al. TRANCE is a novel ligand of the tumor necrosis factor receptor family that activates c-Jun N-terminal kinase in T cells. J Biol Chem. 1997;272(40):25190-4. https://doi.org/10.1074/jbc.272.40.25190
  21. Udagawa N, Takahashi N, Yasuda H, Mizuno A, Itoh K, Ueno Y, et al. Osteoprotegerin produced by osteoblasts is an important regulator in osteoclast development and function. Endocrinology. 2000;141(9):3478-84.
  22. Rani CS, MacDougall M. Dental cells express factors that regulate bone resorption. Mol Cell Biol Res Commun. 2000;3(3):145-52. https://doi.org/10.1006/mcbr.2000.0205
  23. Wise GE, Que BG, Huang H, Lumpkin SJ. Enhancement of gene expression in rat dental follicle cells by parathyroid hormone-related protein. Arch Oral Biol. 2000;45(10):903-9. https://doi.org/10.1016/S0003-9969(00)00053-4
  24. Kartsogiannis V, Zhou H, Horwood NJ, Thomas RJ, Hards DK, Quinn JM, et al. Localization of RANKL (receptor activator of NF kappa B ligand) mRNA and protein in skeletal and extraskeletal tissues. Bone. 1999;25(5):525-34. https://doi.org/10.1016/S8756-3282(99)00214-8
  25. Choi BK, Ohk SH, Lee HJ, Kang JH, Jeong GJ, Yoo YJ. Effects of whole cell sonicates of Treponema lecithinolyticum on osteoclast differentiation. J Periodontol. 2001;72(9):1172-7. https://doi.org/10.1902/jop.2000.72.9.1172
  26. Lee HA, Jeong H, Kim EY, Nam MY, Yoo YJ, Seo JT, et al. Bumetanide, the specific inhibitor of Na+-K+-2Cl- cotransport, inhibits 1alpha,25-dihydroxyvitamin D3-induced osteoclastogenesis in a mouse co-culture system. Exp Physiol. 2003;88(5):569-74. https://doi.org/10.1113/eph8802558