DOI QR코드

DOI QR Code

Liquid Chromatography-Tandem Mass Spectrometric Determination of Geniposide in Rat Plasma and its Pharmacokinetic Application

  • Jin, Ming Ji (Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University) ;
  • Kim, In Sook (Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University) ;
  • Kim, Dong-Hyun (College of Pharmacy, Kyung-Hee University) ;
  • Yoo, Hye Hyun (Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University)
  • 투고 : 2013.01.28
  • 심사 : 2013.06.26
  • 발행 : 2013.09.20

초록

Geniposide is a biologically active ingredient of gardenia fruit. A liquid chromatography-tandem mass spectrometric method was developed and validated for the determination of geniposide in rat plasma. The plasma samples were pretreated by solid-phase extraction and introduced into a BDS Hypersil $C_{18}$ column ($50{\times}2.1mm$, $5{\mu}m$) for chromatographic separation. The mobile phase consisted of 0.1% formic acid and 0.1% formic acid in acetonitrile, and gradient elution was performed at a flow rate of 0.25 mL/min. For mass spectrometric detection, multiple reaction monitoring was performed via an electrospray ionization source in positive mode. The calibration curve for geniposide was linear ($r^2=0.997$) in the concentration range of $0.005-1{\mu}g/mL$. The intra- and inter-day accuracies and precisions fulfilled the required criteria (${\pm}15%$). The developed method was subsequently used for pharmacokinetic analysis of geniposide after oral administration to rats at a dose of 50 mg/kg. The mean maximum plasma concentration of geniposide was $0.68{\pm}0.29{\mu}g/mL$ at $0.44{\pm}0.13h$, and the mean area under the plasma concentration versus time curve was $1.46{\mu}g{\cdot}h/mL$.

키워드

참고문헌

  1. Zhou, T.; Zhao, W.; Fan, G.; Chai, Y.; Wu, Y. J. Chromatogr. B 2007, 858, 296. https://doi.org/10.1016/j.jchromb.2007.08.031
  2. Hsu, H. Y.; Yang, J. J.; Lin, S. Y.; Lin, C. C. Cancer Letters 1997, 113, 31. https://doi.org/10.1016/S0304-3835(96)04572-7
  3. Liu, J.; Yin, F.; Zheng, X.; Jing, J.; Hu, Y. Neurochem. Int. 2007, 51, 361. https://doi.org/10.1016/j.neuint.2007.04.021
  4. Wu, S.; Wang, G.; Liu, Z.; Rao, J.; Lu, L.; Xu, W.; Zhang, J. Acta Pharmacologica Sinica 2009, 30, 202. https://doi.org/10.1038/aps.2008.17
  5. Suzuki, Y.; Kondo, K.; Ikeda, Y.; Umemura, K. Planta Medica 2001, 67, 807. https://doi.org/10.1055/s-2001-18842
  6. Koo, H. J.; Lim, K. H.; Jung, H. J.; Park, E. H. J. Ehnopharmacol. 2006, 103, 496. https://doi.org/10.1016/j.jep.2005.08.011
  7. Lee, M. J.; Hsu, J. D.; Wang, C. J. Anticancer Research 1995, 15, 411.
  8. Sun, Y.; Feng, F.; Yu, X. Phytotherapy Research 2011, 26, 67.
  9. Wang, X.; Lv, H.; Sun, H.; Sun, W.; Liu, L.; Wang, P.; Cao, H. Arzneimittel-Forschung 2008, 58, 336.
  10. Ye, G.; Zhu, H. Y.; Zhao, H. L.; Xu, B.; Huang, C. G. Biomedical Chromatography 2006, 20, 743. https://doi.org/10.1002/bmc.590
  11. Long, Z. M.; Bi, K. S.; Huo, Y. S.; Yan, X. Y.; Zhao, X.; Chen, X. H. J. Sep. Sci. 2011.
  12. Ran, X.; Liang, Q.; Luo, G.; Liu, Q.; Pan, Y.; Wang, B.; Pang, C. J. Cromatogr. B 2006, 842, 22. https://doi.org/10.1016/j.jchromb.2006.05.001
  13. Wang, S. C.; Huang, C. M.; Tsai, T. H. Microchem. J. 2007, 86, 174. https://doi.org/10.1016/j.microc.2007.02.005
  14. US Food and Drug Administration. FDA Guidance for Industry: Bioanalytical Method Validation. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research: Rockville, MD, 2001. Available from: http://www.fda.gov/downloads/Drugs/GuidanceComplianceRegulatoryInformation/Guidances/ucm070107.pdf

피인용 문헌

  1. Effects of Intestinal Microbiota on the Bioavailability of Geniposide in Rats vol.62, pp.40, 2014, https://doi.org/10.1021/jf502557f
  2. A review through recovery, purification and identification of genipin vol.15, pp.1, 2016, https://doi.org/10.1007/s11101-014-9383-z
  3. A Review on the Phytochemistry, Pharmacology, Pharmacokinetics and Toxicology of Geniposide, a Natural Product vol.22, pp.10, 2017, https://doi.org/10.3390/molecules22101689
  4. Plant-Derived Anticancer Agents: Lessons from the Pharmacology of Geniposide and Its Aglycone, Genipin vol.6, pp.2, 2018, https://doi.org/10.3390/biomedicines6020039