Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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The Expression Patterns of Connexin Isoforms in the Rat Caput Epididymis During Postnatal Development

  • Han, Su-Yong (College of Liberal Arts and Sciences, Anyang University) ;
  • Lee, Ki-Ho (Department of Biochemistry and Molecular Biology, College of Medicine, Eulji University)
  • Received : 2013.07.06
  • Accepted : 2013.08.21
  • Published : 2013.08.31
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Abstract

Intercellular interactions are important for the proper development and regulation of tissue function. This is especially necessary in the epididymis, a part of male reproductive tract where sperm become mature and acquire their fertilizing capacity. The caput region of the epididymis consists of several types of cells, including principal, basal, and apical cells. Direct intercellular communication is thus required to precisely regulate the functions of the caput epididymis. In this regard, connexin (Cx) is a molecule that forms channels, which allow the direct exchange of small molecules between cells, enabling intercellular communication. In this study, the expression of Cx isoforms in the caput epididymis at different postnatal ages was determined by using quantitative real-time polymerase chain reaction analysis. Nine of 13 Cx isoforms were detected. The transcript levels of Cx30.3, 31, 31.1, 32, and 40 were highest at 45 days of age, while the expression of Cx43 and 45 gradually decreased with age. A substantial fluctuation of Cx26 expression was detected, with significant decreases before and during puberty, followed by a transient increase at adult-hood and rapid decreases at an old age. A significant increase in Cx37 transcript was observed at 25 days of age, followed by gradual decreases at adult and old ages. These results indicate the significant differential expression of various Cx isoforms in the caput epididymis during postnatal development. It further suggests that the functional regulation and developmental maturation of the caput epididymis are highly related to the postnatal age-related differential expression of Cx isoforms.

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References

  1. Arroteia, K. F., Garcia, P. V., Barbieri, M. F., Justino, M. L. and Pereira, L. A. V. 2012. The epididymis: embryology, structure, function and its role in fertilization and infertility. In: Embryology-Updates and Highlights on Classic Topics. (Ed: L. A. V. Pereira) In Tech. Croatia pp.41-66.
  2. Bosco, D., Haefliger, J. A. and Meda, P. 2011. Connexins: key mediators of endocrine function. Physiol. Rev. 91:1393-1445. https://doi.org/10.1152/physrev.00027.2010
  3. Brown-Grant, K., Fink, G., Greig, F. and Murray, M. A. 1975. Altered sexual development in male rats after oestrogen administration during the neonatal period. J. Reprod. Fertil. 44:25-42. https://doi.org/10.1530/jrf.0.0440025
  4. Cornwall, G. A. 2009. New insights into epididymal biology and function. Hum. Reprod. Update. 15:213-227.
  5. Cyr, D. G., Hermoand, L. and Laird, D. W. 1996. Immunocytochemical localization and regulation of connexin 43 in the adult rat epididymis. Endocrinology. 137:1474-1484. https://doi.org/10.1210/en.137.4.1474
  6. Dube, E., Dufresne, J., Chan, P. T. and Cyr, D. G. 2012. Epidermal growth factor regulates connexin 43 in the human epididymis: role of gap junctions in azoospermia. Hum. Reprod. 27:2285-2296. https://doi.org/10.1093/humrep/des164
  7. Dufresne, J., Finnson, K. W., Gregory, M. and Cry, D. G. 2003. Expression of multiple connexins in the rat epididymis indicates a complex regulation of gap junctional communication. Am. J. Physiol. Cell. Physiol. 284:33-43. https://doi.org/10.1152/ajpcell.00111.2002
  8. Goodenough, D. A. and Paul, D. L. 2009. Gap junctions. Cold Spring Harb. Perspect. Biol. 1:a003061.
  9. Guo, W., Qu, F., Xia, L., Guo, Q., Ying, X. and Ding, Z. 2007. Identification and characterization of ERp29 in rat spermatozoa during epididymal transit. Reproduction. 133:575-584. https://doi.org/10.1530/REP-06-0301
  10. Hannema, S. E. and Hughes, I. A. 2007. Regulation of Wolffian duct development. Hormone Res. 67:142-151. https://doi.org/10.1159/000096644
  11. Hejmej, A., Kotula-Balak, M., Sadowska, J. and Bilinska, B. 2007. Expression of connexin 43 protein in testes, epididymides and prostates of stallions. Equine Vet. J. 39:122-127. https://doi.org/10.2746/042516407X169393
  12. Hermo, L., Chong, D. L., Moffatt, P., Sly, W. S., Waheed, A. and Smith, C. E. 2005. Region- and cell-specific differences in the distribution of carbonic anhydrases II, III, XII, and XIV in the adult rat epididymis. J. Histochem. Cytochem. 53:699-713. https://doi.org/10.1369/jhc.4A6575.2005
  13. Hinton, B. T., Galdamez, M. M., Sutherland, A., Bomgardner, D., Xu, B., Abdel-Fattah, R. and Yang, L. 2011. How do you get six meters of epididymis inside a human scrotum? J. Androl. 32:558-564. https://doi.org/10.2164/jandrol.111.013029
  14. Lasserre, A., Barrozo, S., Tezon, J. G., Miranda, P. V. and Vazquez-Levin, M. H. 2001. Human epididymal proteins and sperm function during fertilization: an update. Biol. Res. 34:165-178.
  15. Lydka, M., Kopera-Sobota, I., Kotula-Balak, M., Chojnacka, K., Zak, D. and Bilinska, B. 2011. Morphological and functional alterations in adult boar epididymis: effects of prenatal and postnatal administration of flutamide. Acta Vet. Scand. 53:12. doi: 10.1186/1751-0147-53-12.
  16. Robaire, B. and Hermo, L. 1988. Efferent ducts, epididymis and vas deferens: structure, functions and their regulation. In: The Physiology of Reproduction. (Eds: E. Knobil and J. Neil) Raven Press. New York pp. 999-1080.
  17. Robaire, B., Syntin, P. and Jervis, K. 2000. The coming of age of the epididymis. In: Testis, Epididymis and Technologies in the Year 2000. (Eds: B. Jegou, C. Pineau and J. Saez) Springer: Hildenberg. New York pp. 229-262.
  18. Robaire, B., Hinton, B. T. and Orgebin-Crist, M. C. 2006. The epididymis. In: Knobil and Neill's Physiology of Reproduction. (Eds: J. D. Neill) Elsevier. New York pp. 1071-1148.
  19. St-Pierre, N., Dufresne, J., Rooney, A. A. and Cyr, D. G. 2003. Neonatal hypothyroidism alters the localization of gap junctional protein connexin 43 in the testis and messenger RNA levels in the epididymis of the rat. Biol. Reprod. 68:1232-1240. https://doi.org/10.1095/biolreprod.102.010504
  20. Sullivan, R. 2004. Male fertility markers, myth or reality. Ani. Reprod. Sci. 82-83:341-347. https://doi.org/10.1016/j.anireprosci.2004.05.007
  21. Sun, E. L. and Flickinger, C. J. 1979. Development of cell types and of regional differentiation in the postnatal rat epididymis. Am. J. Anat. 154:27-55. https://doi.org/10.1002/aja.1001540104