한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제46권4호
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  • Pages.399-406
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  • 2013
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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사료 내 감귤착즙박 첨가가 저수온에서 사육된 참돔(Pagrus major)의 성장, 비특이적 면역반응 및 수온자극 스트레스에 미치는 영향

Effects of Dietary Supplementation of a Citrus By-product on Growth Performance, Innate Immunity and Tolerance of Low Water Temperature in Red Seabream Pagrus major

  • 송진우 (제주대학교 해양생명과학과) ;
  • 박상현 (제주대학교 해양생명과학과) ;
  • 이초롱 (제주대학교 해양생명과학과) ;
  • 이경준 (제주대학교 해양생명과학과)
  • Song, Jin-Woo (Department of Marine Life Sciences, Jeju National University) ;
  • Park, Sang-Hyeon (Department of Marine Life Sciences, Jeju National University) ;
  • Lee, Cho-Rong (Department of Marine Life Sciences, Jeju National University) ;
  • Lee, Kyeong-Jun (Department of Marine Life Sciences, Jeju National University)
  • 투고 : 2013.05.13
  • 심사 : 2013.07.12
  • 발행 : 2013.08.31
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초록

Our aim was to determine the effects of a citrus by-product (CBP) and CBP fermented by Lactobacillus plantarum (LP-CBP), provided as dietary supplements, on the growth performance, feed utilization, innate immunity and temperature tolerance of red seabream. A diet without inclusion of CBP or LP-CBP was used as a control and four other experimental diets were formulated to replace wheat flour by 4% and 8% of either CBP or LP-CBP (designated as Con, LP-CBP4%, LP-CBP8%, CBP4% and CBP8%, respectively). Experimental diets were fed to triplicate groups of 25 fish (initial body weight, 55.0 g) for 9 weeks. Growth performance and feed utilization were not significantly different among all the groups. Bone collagen content was significantly increased by supplementation with CBP and LP-CBP. Vitamin C concentration tended to be higher in livers of fish fed the supplements than in the control group. Myeloperoxidase, lysozyme and superoxide dismutase activities were higher in fish fed CBP or LP-CBP than in fish fed the control diet. When fish were exposed to low water temperature, cumulative mortalities of those fed CBP or LP-CBP supplemented diets were lower (29%, 33%, 34% and 33% mortalities for LP-CBP4%, LP-CBP8%, CBP4% and CBP8%, respectively) than in the control group (58%). Therefore, inclusion of either CBP or LP-CBP at up to 8% in red seabream diet brings benefits through enhanced innate immunity and better tolerance of low water temperature.

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참고문헌

  1. Ai Q, Mai K, Tan B, Xu W, Zhang W, Ma H and Liufu Z. 2006. Effect of dietary vitamin C on survival, growth, and immune of large yellow croaker, Pseudosciaena corcea. Aquaculture 261, 327-336. https://doi.org/10.1016/j.aquaculture.2006.07.027
  2. AOAC. 1995. Official methods of analysis. 16th ed. Association of Official Analytical Chemists, Arlington, Virginia, U.S.A, 1298.
  3. Bae EA, Kwon TW and Moon GS. 1997. Isoflavone contents and antioxidative effects of soybeans, soybean curd and their by products. J Korean Soc Food Nutr 26, 371-375.
  4. Bampidis VA and Robinson PH. 2006. Citrus by-products as ruminant feeds: a review. Anim Feed Sci Tech 128, 175-217. https://doi.org/10.1016/j.anifeedsci.2005.12.002
  5. Braddock RJ. 1983. Utilization of citrus juice vesicle and peel fiber. Food Technol 37, 85-87.
  6. Cha JH, Yang SY, Woo SH, Song JW, Oh DH and Lee KJ. 2012. Effects of dietary supplementation with Bacillus sp. on growth performance, feed utilization, innate immunity and disease resistance against Streptococcus iniae in olive flounder Paralichthys olivaceus. Kor J Fish Aquat Sci. 45, 35-42. https://doi.org/10.5657/KFAS.2012.0035
  7. Cha JY and Cho YS. 2001. Biofunctional activities of citrus flavonoids. J Korean Soc Agric Chem Biotechnol 44, 122-128.
  8. Chatterjee IB. 1978. Ascorbic acid metabolism. World Rev Nutr Diet 30, 69-87.
  9. Choi HS, Park SR and Jung CG. 2002. Biochemical analysis of blood serum from wintering seabream with green liver syndrome. J Fish Pathol 15, 43-48.
  10. Cullen AJ, Harmon DL and Nagaraja TG. 1986. In vitro fermentation of sugars, grains, and by-product feeds in relation to initiation of ruminal lactate production. J Dairy Sci 69, 2616-2621. https://doi.org/10.3168/jds.S0022-0302(86)80709-3
  11. Dabrowski K and Hinterleitner S. 1989. Applications of a simultaneous analysis of ascorbic acid, dehydroascorbic acid and ascorbic sulfate in biological material. Analyst 114, 83-87. https://doi.org/10.1039/an9891400083
  12. Dabrowski K, Matusiewicz M and Blom JH. 1994. Hydrolysis, absorption and bioavailability of ascorbic acid esters in fish. Aquaculture 124, 169-191. https://doi.org/10.1016/0044-8486(94)90376-X
  13. Das S, Ward LR and Burke C. 2008. Prospects of using marine actinobacteria as probiotics in aquaculture. Appl Microbiol Biotechnol 81, 419-429. https://doi.org/10.1007/s00253-008-1731-8
  14. El Naggar GO and Lovell RT. 1991. L-ascorbyl-2-monophosphate has equal antiscorbutic activity as L-ascorbic acid but L-ascorbyl-2-sulfate is inferior to L-ascorbic acid for channel catfish. J Nutr 121, 1622-1626.
  15. Ellis AE. 1990. Serum antiprotease in fish. In: Techniques in fish Immunology. Stolen JS, Fletcher TC, Anderson DP, Roberson WB and Van Muiswinkel WB, eds SOS Publication, Fair Haven, U.S,A., 95-99
  16. Eo J and Lee KJ. 2008. Effect of dietary ascorbic acid on growth and non-specific immune responses of tiger puffer, Takifugu rubripes. Fish Shellfish Immun 25, 611-616. https://doi.org/10.1016/j.fsi.2008.08.009
  17. Fattman CL, Shaefer LM and Oury TD. 2003. Extracellular superoxide dismutase in biology and medicine. Free Radical Bio Med 35, 236-256. https://doi.org/10.1016/S0891-5849(03)00275-2
  18. Folch J, Lee M and Sloane-Stanley GH. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226, 497-509.
  19. Gouillou-Coustans MF, Bergot P and Kaushik SJ. 1998. Dietary ascorbic acid needs of common carp (Cyprinus carpio) larvae. Aquaculture 161, 453-461. https://doi.org/10.1016/S0044-8486(97)00292-5
  20. Grinde B. 1989. Lysozyme from rainbow trout, Salmo gairdneri Richardson, as an antibacterial agent against fish pathogens. J Fish Dis 12, 95-104. https://doi.org/10.1111/j.1365-2761.1989.tb00281.x
  21. Hardie LJ, Fletcher TC and Secombes CJ. 1991. The effect of dietary vitamin C on the immune responses of Atlantic salmon (Salmo salar L.). Aquaculture 95, 201-214. https://doi.org/10.1016/0044-8486(91)90087-N
  22. Iwata N, Kikuchi K, Honda H, Kiyono M and Kurokura H. 1994. Effects of temperature on the growth of Japanese flounder. Fisheries Sci 60, 527-531. https://doi.org/10.2331/fishsci.60.527
  23. Jung YH, Kim DH, Kim HY, Shin TS, Oh MJ, Lee JH, Kim JH, Im SY and Kim E. 2010. Effects of diets supplemented with yuzu Citrus junos Siebold ex Tanaka on disease resistance of olive flounder, Paralichthys olivaceus. J Fish Pathol 23, 389-398.
  24. Kumari J and Sahoo PK. 2005. Effects of cyclophosphamide on the immune system and disease resistance of Asian catfish Clarias batrachus. Fish Shellfish Immun 19, 307-316. https://doi.org/10.1016/j.fsi.2005.01.008
  25. Lee BJ, Kim SS, Song JW, Oh DH, Cha JH, Jeong JB, Heo MS, Kim KW and Lee KJ. 2013. Effects of dietary supplementation of citrus by-products fermented with a probiotic microbe on growth performance, innate immunity and disease resistance against Edwardsiella tarda in juvenile olive flounder, Paralichthys olivaceus (Temminck & Schlegel). J Fish Dis 36, 617-628. https://doi.org/10.1111/jfd.12035
  26. Lin YH, Lin HY and Shiau SY. 2011. Dietary folic acid requirement of grouper, Epinephelus malabaricus, and its effects on non-specific immune responses. Aquaculture 317, 133-137. https://doi.org/10.1016/j.aquaculture.2011.04.010
  27. Lovell RT. 1973. Essentiality of vitamin C in feeds for inten-sively fed caged channel catfish. J Nutr 103, 134-138.
  28. Mead SW and Guilbert HR. 1926. The digestibility of certain fruit by products as determined for ruminants. Part I. Dried orange pulp and raisin pulp. California Agr Exp Sta Bull 409, 1-11.
  29. Navarre O and Halver JE. 1989. Disease resistance and humoral antibody production in rainbow trout fed high levels of vitamin C. Aquaculture 79, 207-221. https://doi.org/10.1016/0044-8486(89)90462-6
  30. Nayak SK. 2010. Review. Probiotics and immunity: A fish Perspective. Fish Shellfish Immun 29, 2-14. https://doi.org/10.1016/j.fsi.2010.02.017
  31. Nobaek S, Johansson ML, Molin G, Ahrne S and Jeppsson B. 2000. Alteration of intestinal microflora is associated with reduction in abdominal bloating and pain in patients with irritable bowel syndrome. Am J Gastroenterol 95, 1231-1238. https://doi.org/10.1111/j.1572-0241.2000.02015.x
  32. Padh H. 1990. Cellular functions of ascorbic acid. Biochem Cell Biol 68, 1166-1173. https://doi.org/10.1139/o90-173
  33. Palic D, Andreasen CB, Menzel BW and Roth JA. 2005. A rapid, direct assay to measure degranulation of primary granules in neutrophils from kidney of fathead minnow (Pimephales promelas Rafinesque, 1820). Fish Shell Immun 19, 217-227. https://doi.org/10.1016/j.fsi.2004.12.003
  34. Panigrahi A, Kiron V, Satoh S, Hirono I, Kobasyashi T, Sugita H, Puangkaew J and Aoki T. 2007. Immune modulation and expression of cytokine genes in rainbow trout Oncorhynchus mykiss upon probiotic feeding. Dev Comp Immunol 31, 372-382. https://doi.org/10.1016/j.dci.2006.07.004
  35. Sankaran K and Gurnani S. 1972. On the variation in catalytic activity of lysozyme in fishes. Indian J Biochem Bio 9, 162-165.
  36. Seo JY, Kim KD, Son MH and Lee SM. 2010. Growth performance, hematological parameter and fatty acid composition of growing olive flounder (Paralichthys olivaceus) to dietary inclusion of kelp meal, krill meal, garlic powder or citrus meal. Kor J Fish Aquat Sci 43, 557-561. https://doi.org/10.5657/kfas.2010.43.5.557
  37. Song YB, Moon SW, Kim SJ and Lee YD. 2002. Effect of EMfermented orange in commercial diet on growth of juvenile flounder, Paralichthys olivaceus. J of Aquaculture 15, 103-110.
  38. Vatassery GT, Smith WE and Quach HT. 1989. Ascorbic acid, glutathione and synthetic antioxidants prevents the oxidation of vitamin E in platelets. Lipids 24, 1043-1047. https://doi.org/10.1007/BF02544077
  39. Wang Y, Xu N, Xi A, Ahmed Z, Zhang B and Bai X. 2009. Effects of Lactobacillus plantrum MA2 isolated from Tibet kefir on lipid metabolism and intestinal microflora of rats fed on high-cholesterol diet. Appl Microbiol Biotechnol 84, 341-347. https://doi.org/10.1007/s00253-009-2012-x
  40. Wilson RP and Poe WE. 1973. Impaired collagen formation in the scorbutic channel catfish. J Nutr 103, 1359-1364.
  41. Wilson RP. 1973. Absence of ascorbic acid synthesis in channel catfish, Ictalurus punctatus, and blue catfish, Ictalurus frucatus. Comp biochem Physiol 46, 635-638.
  42. Xiao LD, Mai KS, Ai QH, Xu W, Wang XJ, Zhang WB and Liufu ZG. 2010. Dietary ascorbic acid requirement of cobia, Rachycentron canadum Linneaus.Aquacult Nutr 16, 582-589. https://doi.org/10.1111/j.1365-2095.2009.00695.x
  43. Zhou Q, Wang L, Wang H, Xie F and Wang T. 2012. Effect of dietary vitamin C on the growth performance and innate immunity of juvenile cobia (Rachycentron canadum). Fish Shell Immun 32, 969-975. https://doi.org/10.1016/j.fsi.2012.01.024

피인용 문헌

  1. Dietary Supplementation of Citrus and Fermented Citrus By-product for Juvenile Red Seabream Pagrus major at Low Water Temperature vol.48, pp.4, 2015, https://doi.org/10.5657/KFAS.2015.0454