The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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The Effect of Gyogam-dan on Depression and Immunity on Repeated Stress in Ovariectomized Rats

교감단이 우울행동과 면역기능에 미치는 효과

  • Cheong, Hyun-Cheol (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Won-Kwang University) ;
  • Kim, Song-Baek (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Won-Kwang University) ;
  • Seo, Yun-Jung (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Won-Kwang University) ;
  • Cho, Han-Baek (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Won-Kwang University) ;
  • Choi, Chang-Min (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Won-Kwang University)
  • 정현철 (원광대학교 한의과대학 한방부인과학교실) ;
  • 김송백 (원광대학교 한의과대학 한방부인과학교실) ;
  • 서윤정 (원광대학교 한의과대학 한방부인과학교실) ;
  • 조한백 (원광대학교 한의과대학 한방부인과학교실) ;
  • 최창민 (원광대학교 한의과대학 한방부인과학교실)
  • Received : 2013.07.15
  • Accepted : 2013.08.19
  • Published : 2013.08.30
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Abstract

Objectives: In this research, the effect of Gyogam-dan (GGD) on depression and immunity were assessed in ovariectomized rats subjected to repetitive stress. GGD is the prescription consisting of Poria cocos and Cyperi Rhizoma. Methods: Ovariectomized rats were repeatedly stressed over a 2-week period. After GGD (100 or 400 mg/kg) were orally administered, Elevated Plus Maze (EPM) and forced swimming test (FST) were performed to evaluate depressive and anxiety response. As well, the change of corticosterone (CORT) and the change of interleukin-$1{\beta}$ (IL-$1{\beta}$) and interleukin-4 (IL-4) in blood serum and in brain were mesured. Results: 1. In the EPM, there were no statistically significant differences among the groups. 2. In the FST, immobility time significantly decreased in rats of each experiment group compared with the control group (p<0.01). 3. Serum CORT level were decreased in 400 mg GGD group (p<0.05). 4. On IL-$1{\beta}$ and IL-4 measurement in the serum and brain, there were not significant increase or decrease compared with the control group. Conclusions: These results suggest that GGD is effective to reduce depression-behavior in ovariectomized rats. However, GGD do not has significant efficacy to reduce anxiety-behavior in EPM test. Measurement of serum CORT level reveals significant decrease and it shows anti-depressant like effect. Results on immunity are not significant.

목 적: 본 연구에서는 부인과의 흔한 질환인 갱년기 우울증의 모델로 반복적인 스트레스를 가한 난소적출 흰쥐를 설정하고 교감단이 쥐의 항우울 행동과 면역기능에 미치는 영향을 살표보고자 하였다. 방 법: 난소적출 흰쥐에 2주간 반복적인 스트레스를 주고, 교감단(100 mg/kg, 400 mg/kg)을 경구 투여한 후 행동검사로 Elevated plus maze, Forced swimming test를 실시하였고, 혈액검사로 혈청 corticosterone, IL-$1{\beta}$와 IL-4의 변화를 측정하였으며, 뇌내의 IL-$1{\beta}$와 IL-4의 변화를 측정하였다. 결 과: 1. Elevated plus maze에서 교감단 100 mg/kg 투여군은 open arms에서의 보낸시간이 대조군에 비해서 차이가 없었으며, 교감단 400 mg/kg 투여군은 open arms에서 보낸 시간이 대조군에 비하여 증가되었으나 유의성은 없었다. 2. Elevated plus maze에서 교감단 100 mg/kg 투여군은 crossing 횟수가 대조군에 비하여 차이가 없었으며, 교감단 400 mg/kg 투여군은 crossing 횟수가 대조군에 비하여 늘어났으나 유의성은 없었다. 3. Forced swimming test에서 교감단 100 mg/kg, 400 mg/kg 투여군은 각각 대조군에 비해서 immobility 시간이 유의성 있게 감소하였다(p<0.01). 4. Corticosterone 측정에서 교감단 400 mg/kg을 투여한 후 Corticosterone 수준이 유의하게 감소하였다(p<0.05). 5. 혈청내 IL-$1{\beta}$와 IL-4 측정에서 교감단 투여군은 대조군에 비해 유의성 있는 감소나 증가가 관찰되지 않았다. 6. 뇌내 IL-$1{\beta}$와 IL-4 측정에서 교감단 투여군은 대조군에 비해 유의성 있는 감소나 증가가 관찰되지 않았다. 결 론: 이상의 결과를 보면 교감단은 난소적출 흰쥐의 우울행동의 완화에 유의성 있는 결과를 나타내었으며, 불안 행동검사에서는 유효한 효과가 없었다. 혈청 corticosterone 측정에서 유의성 있는 감소를 나타내어 항우울효과를 나타내었으나, 면역기능에 작용하는 유의성 있는 결과는 관찰되지 않았다.

Keywords

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