DOI QR코드

DOI QR Code

Diagnostic Calculation of Trace Calcium Ions in Food Using a DNA doped Sensor

  • Yang, Young-Kyun (Department of Basic Education Seoul National University of Technology) ;
  • Ly, Suw-Young (Biosensor Research Institute in Seoul National University of Technology)
  • Received : 2013.03.14
  • Accepted : 2013.06.28
  • Published : 2013.06.30

Abstract

The diagnostic assay of calcium ion was sought using a modified sensor with square-wave stripping voltammetry (SWSV) and cyclic voltammetry (CV). In this study, simple graphite pencil was used as working, reference, and auxiliary electrodes. By coating the working electrodes with DNA, their sensitivity was very much improved, and good results were yielded. Moreover, clean seawater was used as an electrolyte solution instead of acid and base electrolytes to lessen the expenses involved in the experiment. The analytical optimum conditions were also examined. These conditions were attained at the low detection limit of $0.6ugL^1$. After that, the results were applied to drinking water of milk contain.

Keywords

References

  1. W J Lee, G R. Monteith, Sarah J. R Thomson, Calcium transport and signaling in the mammary gland:Targets for breast cancer, Biochimica et Biophysica Acta 235 (2006)
  2. J. H. Kleibeuker, R. V Meer, E.G.E. Vries, Calcium and Vitamin D: Possible Protective Agents Against Colorectal Cancer, European journal of Cancer.718, 1081 (1995)
  3. I N. Sergeev, Calcium signaling in cancer and vitamin D, Journal of Steroid Biochemistry & Molecular Biology 97 145 (2005)
  4. J. V Salas, P. B-Palau, C. P Felis, S. M Garcia, S. P-Holanda, Calcium Inhibits Colon Carcinogenesis in an Experimental Model in the Rat, European Journal of Cancer, 34,. 1941 (1998) https://doi.org/10.1016/S0959-8049(98)00197-X
  5. H S Park, C J Kim, Effect of dietary fat and calcium level on the biomarkers of dimethylhydrazinetreated rats, Nutrition Research 22 365 (2002) https://doi.org/10.1016/S0271-5317(01)00394-3
  6. CY Yang, S S Tsai, T C Lai, C F Hung, H F Chiu, Rectal Cancer Mortality and Total Hardness Levels in Taiwan''s Drinking Water, Environmental Research Section A 80, 311 (1999) https://doi.org/10.1006/enrs.1998.3921
  7. A M. Beccaglia, C A. Rinaldi, J C. Ferrero, Analysis of arsenic and calcium in soil samples by laser ablation mass spectrometry, Analytica Chimica Acta 11 (2006)
  8. J.F. van Staden, R.E. Taljaard, Determination of calcium in water, urine and pharmaceutical samples by sequential injection analysis, Analytica Chimica Acta 75 (1996)
  9. J Kaloustian, T F.Moselhy, H Portugal, Determination of calcium oxalate (monoand dihydrate) in mixtures with magnesium ammonium phosphate or uric acid: the use of simultaneous thermal analysis in urinary calculi, Clinica Chimica Acta 117 (2003)
  10. I I. Maes, G Gryglewicz, J Yperman, D V. Franco, J Mullens, C. V Poucke, Effect of calcium and calcium minerals in coal on its thermal analysis, 76, 143 (1997)
  11. M Trojanowicza, P W. Alexanderb, D. B Hibbert, Flow-injection analysis wit potentiometric detection for the speciation of uoride and calcium, Analytica Chimica Acta 366 23 (1998) https://doi.org/10.1016/S0003-2670(97)00637-5
  12. C. F. Lin, C.H. Wang, W. Y. Liao, T.C. Chou- B. Lee, Hydrogen and calcium ion electrochemical detecting systems using microfluidic technology, Micro & Nano Letters, 1 29 (2006) https://doi.org/10.1049/mnl:20065031
  13. N.V. Vagenas, A. Gatsouli, C.G. Kontoyannis, Quantitative analysis of synthetic calcium carbonate polymorphs using FT-IR spectroscopy, Talanta 59 831 (2003) https://doi.org/10.1016/S0039-9140(02)00638-0
  14. J Nyman, A Ivaska, Spectrophotometric determination of calcium in paper machine white water by sequential injection analysis, Analytica Chimica Acta 308 286 (1995) https://doi.org/10.1016/0003-2670(94)00476-3
  15. R. Traversi, S. Becagli, E. Castellano, V. Maggi, A. Morganti , M. Severi, R. Udisti, Ultra-sensitive Flow Injection Analysis (FIA) determination of calcium in ice cores at ppt level, Analytica Chimica Acta 594 219 (2007) https://doi.org/10.1016/j.aca.2007.05.022
  16. Y Nie, J Lv, Utilizing multibubble sonophotoluminscence for calcium quantitative analysis in pharmaceuticals, Ultrasonics Sonochemistry 15 344 (2008) https://doi.org/10.1016/j.ultsonch.2007.07.011
  17. G Jo, M S Won, Y B Shim, Amperometric Determination of Calcium Ion with an Alizarin /Nafion Modified Electrode, Department of Chemistry and Chemistry Institute of Functional Materials, Pusan National University, Pusan 609, Korea
  18. J Zheng, Y Zhang, P Yang,, An ionic liquid-type carbon paste electrode for electrochemical investigation and determination of calcium dobesilate, Talanta 73, 920 (2007) https://doi.org/10.1016/j.talanta.2007.05.016
  19. A Kumar, S. K. Mittal, PVC based dibenzo-18-crown-6 electrode for Ca(II) ions , Sensors and Actuators 340 (2004)
  20. X Zhang, S Wang, L Jia, Z Xu, Y Zeng, "An electrochemical sensor for determination of calcium dobesilate based on PoPD/MWNTs composite film modified glassy carbon electrode", J. Biochem. Biophys. Methods, 70, 1203 (2008) https://doi.org/10.1016/j.jbbm.2007.10.002