생명과학회지 (Journal of Life Science)

  • 제23권8호
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  • Pages.1032-1040
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  • 2013
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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발효 동충하초의 이화학적 특성

Chemical Characteristics in Fermented Cordycepin-enriched Cordyceps militaris

  • 안희영 (동아대학교 대학원 의생명과학과) ;
  • 박규림 (동아대학교 대학원 의생명과학과) ;
  • 김유라 (동아대학교 생명공학과) ;
  • 차재영 (대선주조(주) 기술연구소) ;
  • 조영수 (동아대학교 생명공학과)
  • Ahn, Hee-Young (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Park, Kyu-Rim (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Kim, Yu-Ra (Department of Biotechnology, Dong-A University) ;
  • Cha, Jae-Young (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Cho, Young-Su (Department of Biotechnology, Dong-A University)
  • 투고 : 2013.08.21
  • 심사 : 2013.08.28
  • 발행 : 2013.08.30
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초록

유용발효미생물에 의한 cordycepin 고함유 동충하초 분말의 생리활성 작용 및 물질을 개발하기 위한 기초 자료를 얻을 목적으로 2 종의 유산균 Lactobacillus hilgardii (Lh), Lactobacillus acidophilus (La)와 1 종의 효모 Saccharomyces cerevisiae (Sc), 1 종의 바실러스 Bacillus subtilis (Bs), 4 종의 곰팡이 Aspergillus oryzae (Ao), Aspergillus kawachii (Ak) 및 Monascus purpureus (Mp), Rhizopus oryzae (Ro)를 본 실험에 사용하였으며, 이들에 의한 발효 cordycepin 고함유 동충하초 분말의 미네랄 함량, 지방산 조성, 단백질 함량, 단백질 패턴, 항산화 활성, 혈전용해 활성을 비교 검토하였다. 발효 cordycepin 고함유 동충하초분말의 주요 미네랄은 미네랄 성분 조성은 K, Mg, Ca, Na, Fe, Zn로 나타났으며, 전체적으로 K, Mg, Ca 순으로 높은 비율을 차지하였고, 주요 지방산 성분으로 palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid 이다. 동충하초 분말과 cordycepin 고함유 동충하초분말 및 발효시킨 cordycepin 고함유 동충하초 분말 단백질을 native-PAGE로 비교분석 한 결과 발효에 의해 균주에 상관없이 특정 부분의 단백질 분해를 확인하였고, SDS-PAGE 상의 단백질 패턴 분석에서는 66 kDa 크기의 단백질 밴드가 유산균 발효에 의해 분해 되어 거의 관찰되지 않았으며, Aspergillus oryzae에서 새로운 단백질 밴드를 확인하였다. Cordycepin 고함유 동충하초 분말을 유용미생물로 발효시킨 결과 Bacillus subtilis 균주 발효에서 혈전용해 활성이 17.1 unit으로 높게 나왔으며, Lactobacillus hilgardii 균주 발효에 의해서는 7.2 unit으로 낮은 혈전용해 활성이 나왔다. 항산화 활성은 발효시킨 cordycepin 고함유 동충하초 분말에서 균주 종류에 관계없이 거의 비슷하게 나왔지만 시판 항산화제 BHT 처리구의 90% 이상의 높은 항산화 활성보다는 낮았다. 이상의 실험결과에서 유용미생물 발효에 의한 cordycepin 고함유 동충하초 분말의 이화학적 특성이 발효 전 cordycepin 고함유 동충하초 분말보다 강화됨으로써 건강식품의 소재로서의 이용 가능성이 높다고 할 수 있다.

The comparative effects of fibrinolytic, antioxidative activity and electrophoretical protein patterns with Cordyceps militaris powder (CM) and cordycepin-enriched Cordyceps militaris JLM0636 powder (CCM) and fermented cordycepin-enriched Cordyceps militaris JLM0636 powder by several microscopic organisms were investigated. In addition, nutritional materials such as mineral, protein, and fatty acids were also measured. The protein concentration was higher in CCM than that in CM. The protein concentration in fermented CCM was the highest in CCM treated by Aspergillus kawachii among the various samples. When total protein patterns of CM, CCM, and both fermented CCMs were analyzed by native- and SDS-PAGE, there were slightly varietal differences in electrophoretical protein patterns. Major minerals were K, Ca, Mg, and Zn. Major fatty acids were palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid. Fibrinolytic activity was the highest in the fermented CCM by Bs treatment among the various samples. The ${\alpha},{\alpha}^{\prime}$-diphenyl-${\beta}$-picrylhydrazyl (DPPH) radical scavenging activity was slightly stronger in the CCM treated with Aspergillus kawachii among the various samples; however, these samples all exhibited relatively low levels of activity compared with the butylated hydroxytoluene (BHT). These results may provide the basic data necessary to understand the biological activities and chemical characteristics of Cordyceps militaris JLM0636 powder fermented by several microscopic organisms to develop functional foods.

키워드

참고문헌

  1. A.O.A.C. 1975. Official methods of analysis. 12th ed., Association of official analytical chemists. Washington, DC, USA.
  2. Astrup, T. and Mullertz, S. 1991. The fibrin plate method for estimating fibrinolytic activity. Arch Biochem Biophys 40, 346-351.
  3. Bate-Smith, E. C. 1954. Flavonoid compounds in foods. Adv food Res 5, 216.
  4. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1204.
  5. Bok, J. W., Ller, J. C., Klin, H. G. and Neil, G. H. 1999. Antitumor sterols from the mycelia of Cordyceps sinensis. Phytochemistry 51, 891-898. https://doi.org/10.1016/S0031-9422(99)00128-4
  6. Cabras, P. and Angioni, A. 2000. Pesticide residues in grapes, wine, and their processing products. J Agric Food Chem 48, 967-973. https://doi.org/10.1021/jf990727a
  7. Cha, J. Y., Kim, H. J., Chung, C. H. and Cho, Y. S. 1999. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J Korean Soc Food Sci Nutr 28, 1310-1315.
  8. Cha, J. Y., Yang, H. J., Jeong, J. J., Seo, W. S., Park, J. S., Ok, M. and Cho, Y. S. 2010. Tyrosinase inhibition activity and antioxidant capacity by fermented products of some medicinal plants. J Life Sci 20, 940-947. https://doi.org/10.5352/JLS.2010.20.6.940
  9. Cha, J. Y., Park, J. C., Ahn, H. Y., Eom, K. E., Park, B. K., Jun, B. S. and Cho, Y. S. 2009. Effect of Monascus purpureus fermented Korean red ginseng powder on the serum lipid levels and antioxidative activity in rats. J Korean Soc Food Sci Nutr 38, 1153-1160. https://doi.org/10.3746/jkfn.2009.38.9.1153
  10. Cha, J. Y., Cho, Y. S., Kim, I., Anno, T., Rahman, S. M. and Yanagita, T. 2001. Effect of hesperetin, a citrus flavonoid, on the liver triacylglycerol content and phosphatidate phosphohydrolase activity in orotic acid-fed rats. Plant Foods Human Nutr 56, 349-358. https://doi.org/10.1023/A:1011884200848
  11. Cha, J. Y., Kim, Y. S., Ahn, H. Y., Eom, K. E., Park, B. K. and Cho, Y. S. 2009. Biological activity of fermented silkworm powder. J Life Sci 19, 1468-1477. https://doi.org/10.5352/JLS.2009.19.10.1468
  12. Cho, H. J., Cho, J. Y., Rhee, M. H., Kim, H. S., Lee, H. S. and Park, H. J. 2007. Inhibitory effects of cordycepin (3'-deoxyadenosine), a component of Cordyceps militaris, on human platelet aggregation induced by thapsigargin. J Microbiol Biotechnol 17, 1134-1138.
  13. Choi, H. J., Lee, W. S., Hwang, S. J., Lee, I. J., Shin, D. H., Kim, H. Y. and Kim, K. U. 2000. Changes in chemical compositions of green tea under the different extraction conditions. Korean J Life Sci 10, 202-209.
  14. Choi, Y. M., Gu, J. B., Kim, M. H. and Lee, J. S. 2008. Antioxidant and antiproliferative activities of methanolic extracts from thirty Korean medicinal plants. Food Sci Biotechnol 17, 1235-1239.
  15. Davis, B. J. 1964. Disc electrophoresis-II. Method and application to human serum proteins. Ann N Y Acad Sci 28, 404-427.
  16. Duncan, D. B. 1959. Multiple range and multiple F test. Biometrics 1, 1-42.
  17. Fuller, R. 1991. Probiotics in human medicine. Gut 32, 439-442. https://doi.org/10.1136/gut.32.4.439
  18. Garces, R. and Mancha, M. 1993. One-step lipid extraction and fatty acid methyl esters preparation from fresh plant tissues. Anal Biochem 211, 139-143. https://doi.org/10.1006/abio.1993.1244
  19. Jia, Z., Tang, M. and Wu, J. 1999. The determination of flavonoid contents in mulberry and thier scavenging effects on superoxide radicals. Food Chem 64, 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
  20. Kang, P. D., Yoon, H. J., Ryu, K. S., Sohn, B. H. and Sohn, H. D. 1999. Electrophoretic patterns of hemolymph proteins of varieties with long and short life span in the silkworm Bombyx mori L. Korean J Seric Sci 41, 1-8.
  21. Kim, D. H., Han, S. B., Park, J. S., Han, M. J. 1994. Fermentation of antler and its biological activity. Korean J Pharmacogn 25, 233-237.
  22. Kim, I. J., Kim, H. K., Jong, J. H., Jeong, Y. K. and Ryu, C. H. 2002. Study of functional Chungkukang contain fibrinolytic enzyme. Korean J Life Sci 12, 357-362. https://doi.org/10.5352/JLS.2002.12.3.357
  23. Kwon, H. J., Lee, K. H., Kim, J. H., Chun, S. S., Cho, Y. J. and Cha, W. S. 2006. Effect of protease on the extraction and properties of the protein from silkworm pupa. J Korean Soc Appl Biol Chem 49, 304-308.
  24. Li, S. P., Zhang, G. H., Zeng, Q., Huang, Z. G., Wang, Y. T., Dong, T. T. and Tsim, K. W. 2006. Hypoglycemic activity of polysaccharide, with antioxidation, isolated from cultured Cordyceps mycelia. Phytomedicine 13, 428-433. https://doi.org/10.1016/j.phymed.2005.02.002
  25. Lowry, O. H., Rosebrogh, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265-271.
  26. Luthringer, C., Rayssiguier, Y., Gueux, E. and Berthelot, A. 1988. Effect of moderate magnesium deficiency on serum lipids, blood pressure and cardiovascular reactivity in normotensive rats. Br J Nutr 59, 243-250. https://doi.org/10.1079/BJN19880031
  27. Muller, W. E., Weiler, B. E., Charubala, R., Pfleiderer, W., Leserman, L., Sobol, R. W., Suhadolnik, R. J. and Schroder, H. C. 1991. Cordycepin analogues of 2', 5'-oligo-adenylate inhibition of reverse transcriptase. Biochemistry 30, 2027-2033. https://doi.org/10.1021/bi00222a004
  28. Owens, C. W. I. and Belcher, R. V. 1965. A colorimetric micro- method for the determination of glutathione. Biochem J 94, 705-711.
  29. Sung, J. M., Choi, Y. S., Lee, H. K., Kim, S. H., Kim, Y. O. and Sung, G. H. 1999. Production of fruitingbody using cultures of Entomopathogenic fungal species. Korean J Mycol 27, 15-19.
  30. Swain, T., Hillis, W. E. and Oritega, M. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. J Sci Food Agric 10, 83-88.
  31. Tsukahara, M., Shinzato, N., Tamaki, Y., Namihira, T. and Matsui, T. 2009. Red yeast rice fermentation by selected Monascus sp. with deep-red color, lovastatin production but no citrinin, and effect of temperature-shift cultivation on lovastatin production. Appl Biochem Biotechnol 158, 476-482. https://doi.org/10.1007/s12010-009-8553-8
  32. Weber, K. and Osborn, M. 1969. The reliability of molecular weight determination by sodium dodesyl sulphate-polyacrylamide gel electrophoresis. J Biol Chem 244, 4406-4412.
  33. Sugimura, Y. and Yamamoto, K. 1998. The protective effect of glutathione-enriched yeast extract on acetaminophen-induced liver damage in rats. J Jpn Soc Nutr Food Sci 51, 189-193. https://doi.org/10.4327/jsnfs.51.189
  34. Yun, J. S. 2009. Pathomorphological peculiarity of dometic silkworm, Bombyx mori. infected with Cordyceps, Paecilomyces tenuipes. Korean J Pesticide Sci 13, 177-184.
  35. Zhu, J. S., Halpern, G. M. and Jones, K. 1998. The scientific rediscovery of an ancient Chinese herbal medicine, Cordyceps sinensis Part I. J Altern Complement Med 4, 289-303. https://doi.org/10.1089/acm.1998.4.3-289

피인용 문헌

  1. Biological Activity and Chemical Characteristics of Cordyceps militaris Powder Fermented by Several Microscopic Organisms vol.25, pp.2, 2015, https://doi.org/10.5352/JLS.2015.25.2.197
  2. Microbiological and Physicochemical Quality Characteristics of Raw Noodle with Natural Food Preservatives vol.26, pp.5, 2016, https://doi.org/10.17495/easdl.2016.10.26.5.435