Journal of Korean Dental Science

Korean Academy of Dental Science (대한치의학회)
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Transglutaminase 2 mRNA Expression in Salivary Gland Tumor Cell Line

  • Chun, Yoon Kwon (Department of Oral Pathology, The Oral Aging Research Center, College of Dentistry, Dankook University) ;
  • Lee, Chong Heon (Department of Oral Pathology, The Oral Aging Research Center, College of Dentistry, Dankook University)
  • Received : 2013.06.07
  • Accepted : 2013.06.14
  • Published : 2013.06.30
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Abstract

Purpose: Transglutaminase 2 (TGase 2) is expressed by tumor necrosis factor-${\alpha}$ in various carcinoma. The role of TGase 2 expression in salivary gland tumors is not clear yet. Established slaivary gland tumor (SGT)cell line has been used to study the pathogenesis of salivary gland adenocarcinoma on a cellular level in vitro. The pupose of this study were to examine mRNA expression of TGase 2 in SGT cell line compared to other tumor cell lines, and to apply these results to the pathogenesis of salivary gland tumor. Materials and Methods: After SGT, SCC-15, HN 4, and HeLa tumor cell lines were cultured under preconfl uency, and 3 days after postconfl uency, the cells were harvested for total RNA extraction and cDNA preparation. Result: Reverse transcription polymerase chain reaction for semiquantitative mRNA analysis was done. TGase 2 mRNA expression was not induced by confl uency in all the cell lines. TGase 2 mRNA expression was variable but markedly enhanced in SGT cell line. Conclusion: mRNA expression of TGase 2 should play an important role in the pathogenesis of SGT cell line originated from ductal cell.

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References

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