Current Research on Agriculture and Life Sciences
- 제31권2호
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- Pages.94-100
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- 2013
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- 2287-271X(pISSN)
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- 2288-0356(eISSN)
Efficient Plant Regeneration from Petal Segment of Standard-Type Chrysanthemum
- Chhetri, Mahesh (Department of Horticultural Science, College of Agriculture and Life Science, Kyungpook National University) ;
- Jeon, Su-Min (Department of Horticultural Science, College of Agriculture and Life Science, Kyungpook National University) ;
- Naing, Aung Htay (Department of Horticultural Science, College of Agriculture and Life Science, Kyungpook National University) ;
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Kim, Chang Kil
(Department of Horticultural Science, College of Agriculture and Life Science, Kyungpook National University)
- 투고 : 2013.06.04
- 심사 : 2013.06.30
- 발행 : 2013.06.30
초록
An efficient plant regeneration protocol is developed for a standard-type chrysanthemum. When petal segments derived from flower buds (4 or 8cm in diameter) were used as the culture material, the highest shoot regeneration frequency (96%) was obtained on a Murashige and Skoog (MS) medium supplemented with 0.5 mg/L IAA, 2 mg/L BA, 3% sucrose, and a 0.8% agar. Pre-culturing the explants under dark conditions for 14 days produced better results for the shoot regeneration frequency than the explants cultured under a continuous 16 h photoperiod (