Nutrition Research and Practice

  • 제7권3호
  • /
  • Pages.160-165
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  • 2013
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  • 1976-1457(pISSN)
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  • 2005-6168(eISSN)
한국영양학회 (The Korean Nutrition Society)
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Peanut sprout ethanol extract inhibits the adipocyte proliferation, differentiation, and matrix metalloproteinases activities in mouse fibroblast 3T3-L1 preadipocytes

  • Kim, Woo Kyoung (Department of Food Science and Nutrition, Dankook University) ;
  • Kang, Nam E (Department of Food and Nutrition, Eulji University) ;
  • Kim, Myung Hwan (Department of Food Engineering, Dankook University) ;
  • Ha, Ae Wha (Department of Food Science and Nutrition, Dankook University)
  • 투고 : 2013.01.28
  • 심사 : 2013.03.19
  • 발행 : 2013.06.01
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초록

3T3-L1 preadipocyte were differentiated to adipocytes, and then treated with 0, 10, 20, and $40{\mu}g/mL$ of peanut sprout ethanol extract (PSEE). The main component of PSEE is resveratrol which contained 5.55 mg/mL of resveratrol. The MTT assay, Oil-Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity, and the triglyceride concentration were determined in 3T3-L1 cells. MMP-2 and MMP-9 activities as well as mRNA expressions of C/EBP ${\beta}$ and C/EBP ${\alpha}$ were also investigated. As the concentration of PSEE in adipocytes increased, the cell proliferation was decreased in a dose-dependent manner from 4 days of incubation (P < 0.05). The GDPH activity (P < 0.05) and the triglyceride concentration (P < 0.05) were decreased as the PSEE treatment concentration increased. The mRNA expression of C/EBP${\beta}$ in 3T3-L1 cells was significantly low in groups of PSEE-treated, compared with control group (P < 0.05). The MMP-9 (P < 0.05) and MMP-2 (P < 0.05) activities were decreased in a dose-dependent manner as the PSEE concentration increased from $20{\mu}g/mL$. In conclusion, it was found that PSEE has an effect on restricting proliferation and differentiation of adipocytes.

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참고문헌

  1. Cornelius P, MacDougald OA, Lane MD. Regulation of adipocyte development. Annu Rev Nutr 1994;14:99-129. https://doi.org/10.1146/annurev.nu.14.070194.000531
  2. Camp HS, Ren D, Leff T. Adipogenesis and fat-cell function in obesity and diabetes. Trends Mol Med 2002;8:442-7. https://doi.org/10.1016/S1471-4914(02)02396-1
  3. Rosen ED, Walkey CJ, Puigserver P, Spiegelman BM. Transcriptional regulation of adipogenesis. Genes Dev 2000;14:1293-307.
  4. Bouloumie A, Sengenes C, Portolan G, Galitzky J, Lafontan M. Adipocyte produces matrix metalloproteinases 2 and 9: involvement in adipose differentiation. Diabetes 2001;50:2080-6. https://doi.org/10.2337/diabetes.50.9.2080
  5. Bhat KP, Pezzuto JM. Cancer chemopreventive activity of resveratrol. Ann N Y Acad Sci 2002;957:210-29. https://doi.org/10.1111/j.1749-6632.2002.tb02918.x
  6. de la Lastra CA, Villegas I. Resveratrol as an antioxidant and pro-oxidant agent: mechanisms and clinical implications. Biochem Soc Trans 2007;35:1156-60. https://doi.org/10.1042/BST0351156
  7. Das S, Alagappan VK, Bagchi D, Sharma HS, Maulik N, Das DK. Coordinated induction of iNOS-VEGF-KDR-eNOS after resveratrol consumption: a potential mechanism for resveratrol preconditioning of the heart. Vascul Pharmacol 2005;42:281-9. https://doi.org/10.1016/j.vph.2005.02.013
  8. Kopp P. Resveratrol, a phytoestrogen found in red wine. A possible explanation for the conundrum of the 'French paradox'? Eur J Endocrinol 1998;138:619-20. https://doi.org/10.1530/eje.0.1380619
  9. Hardie DG, Pan DA. Regulation of fatty acid synthesis and oxidation by the AMP-activated protein kinase. Biochem Soc Trans 2002;30:1064-70. https://doi.org/10.1042/bst0301064
  10. Szkudelska K, Nogowski L, Szkudelski T. Resveratrol, a naturally occurring diphenolic compound, affects lipogenesis, lipolysis and the antilipolytic action of insulin in isolated rat adipocytes. J Steroid Biochem Mol Biol 2009;113:17-24. https://doi.org/10.1016/j.jsbmb.2008.11.001
  11. Dal-Pan A, Blanc S, Aujard F. Resveratrol suppresses body mass gain in a seasonal non-human primate model of obesity. BMC Physiol 2010;10:11. https://doi.org/10.1186/1472-6793-10-11
  12. Gu X, Creasy L, Kester A, Zeece M. Capillary electrophoretic determination of resveratrol in wines. J Agric Food Chem 1999;47:3223-7. https://doi.org/10.1021/jf981211e
  13. Wang KH, Lai YH, Chang JC, Ko TF, Shyu SL, Chiou RY. Germination of peanut kernels to enhance resveratrol biosynthesis and prepare sprouts as a functional vegetable. J Agric Food Chem 2005;53:242-6. https://doi.org/10.1021/jf048804b
  14. Kang HI, Kim JY, Park KW, Kang JS, Choi MR, Moon KD, Seo KI. Resveratrol content and nutritional components in peanut sprouts. Korean J Food Preserv 2010;17:384-90.
  15. Kang HI, Kim JY, Kwon SJ, Park KW, Kang JS, Seo KI. Antioxidative effects of peanut sprout extracts. J Korean Soc Food Sci Nutr 2010;39:941-6. https://doi.org/10.3746/jkfn.2010.39.7.941
  16. Lin BS, Lien TF, Chao MR, Lai TY, Chang JC, Chou SJ, Liao HF, Chiou RY. Toxicological and nutraceutical assessments of peanut sprouts as daily supplements to feed Sprague-Dawley rats for 18 weeks. J Sci Food Agric 2008;88:2201-7. https://doi.org/10.1002/jsfa.3335
  17. Green H, Kehinde O. An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion. Cell 1975;5:19-27. https://doi.org/10.1016/0092-8674(75)90087-2
  18. Lee MS, Kim CT, Kim CJ, Cho YJ, Kim Y. Effects of Portulaca oleracea L. extract on lipolysis and hormone sensitive lipase (HSL) gene expression in 3T3-L1 adipocytes. Korean J Nutr 2006;39: 742-7.
  19. Kim MJ, Kim Y, Chung JH, Kim JW, Kim HK. The effect of caffeine on 3T3-L1 adipocyte differentiation: a nutrigenomical approach. Korean J Nutr 2005;38:649-55.
  20. Chon JW, Sung JH, Hwang EJ, Park YK. Chlorella methanol extract reduces lipid accumulation in and increases the number of apoptotic 3T3-L1 cells. Ann N Y Acad Sci 2009;1171:183-9. https://doi.org/10.1111/j.1749-6632.2009.04895.x
  21. Tomiyama K, Nakata H, Sasa H, Arimura S, Nishio E, Watanabe Y. Wortmannin, a specific phosphatidylinositol 3-kinase inhibitor, inhibits adipocytic differentiation of 3T3-L1 cells. Biochem Biophys Res Commun 1995;212:263-9. https://doi.org/10.1006/bbrc.1995.1965
  22. Ramírez-Zacarías JL, Castro-Muñozledo F, Kuri-Harcuch W. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 1992;97:493-7. https://doi.org/10.1007/BF00316069
  23. Na MH, Seo EY, Kim WK. Effects of $\alpha$-lipoic acid on cell proliferation and apoptosis in MDA-MB-231 human breast cells. Nutr Res Pract 2009;3:265-71. https://doi.org/10.4162/nrp.2009.3.4.265
  24. Kwon SY, Kang KJ. The effect of conjugated linoleic acid isomers on the cell proliferation, apoptosis and expressions of uncoupling protein (UCP) genes during differentiation of 3T3-L1 preadipocytes. Korean J Nutr 2004;37:533-9.
  25. Kyoya T, Ishida A, Nakashima K, Nakajima I, Toyoda A, Nakamura Y, Katsumata M. The effects of concentrations of lysine in media on differentiation of 3T3-L1 preadipocytes. Anim Sci J 2011;82:565-70. https://doi.org/10.1111/j.1740-0929.2011.00882.x
  26. Olofsson LE, Orho-Melander M, William-Olsson L, Sjoholm K, Sjöström L, Groop L, Carlsson B, Carlsson LM, Olsson B. CCAAT/enhancer binding protein alpha (C/EBPalpha) in adipose tissue regulates genes in lipid and glucose metabolism and a genetic variation in C/EBPalpha is associated with serum levels of triglycerides. J Clin Endocrinol Metab. 2008;93:4880-6. https://doi.org/10.1210/jc.2008-0574
  27. Cho KJ, Moon HE, Moini H, Packer L, Yoon DY, Chung AS. Alpha-lipoic acid inhibits adipocyte differentiation by regulating pro-adipogenic transcription factors via mitogen-activated protein kinase pathways. J Biol Chem 2003;278:34823-33. https://doi.org/10.1074/jbc.M210747200
  28. Hong MK, Cho KY, Oh SJ, Kim KM, Yu SJ, Jung SS. Implications of the activation of matrix metalloproteinase-2 (MMP-2) on the metastasis in breast cancer. J Korean Surg Soc 2002;62:18-25.
  29. Rural Development Administration, National Institute of Crop Science [Internet]. Suwon: Rural Development Administration; 2010 [cited 2010 October 14]. Available from: http://rda.korea.kr/gonews.
  30. Shin HD. Anti-obesity effect of vegetable sprouts in 3T3-L1 adipocytes and rats [master's thesis]. Gwangju: Chosun University; 2007.
  31. Wise LS, Green H. Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells. J Biol Chem 1979;254:273-5.
  32. Rayalam S, Yang JY, Ambati S, Della-Fera MA, Baile CA. Resveratrol induces apoptosis and inhibits adipogenesis in 3T3-L1 adipocytes. Phytother Res 2008;22:1367-71. https://doi.org/10.1002/ptr.2503
  33. Zhang XH, Huang B, Choi SK, Seo JS. Anti-obesity effect of resveratrol-amplified grape skin extracts on 3T3-L1 adipocytes differentiation. Nutr Res Pract 2012;6:286-93. https://doi.org/10.4162/nrp.2012.6.4.286
  34. Kang NE, Ha AW, Kim JY, Kim WK. Resveratrol inhibits the protein expression of transcription factors related adipocyte differentiation and the activity of matrix metalloproteinase in mouse fibroblast 3T3-L1 preadipocytes. Nutr Res Pract 2012;6:499-504. https://doi.org/10.4162/nrp.2012.6.6.499
  35. Park HJ, Yang JY, Ambati S, Della-Fera MA, Hausman DB, Rayalam S, Baile CA. Combined effects of genistein, quercetin, and resveratrol in human and 3T3-L1 adipocytes. J Med Food 2008;11:773-83. https://doi.org/10.1089/jmf.2008.0077
  36. Folkman J, Klagsbrun M. Angiogenic factors. Science 1987;235:442-7. https://doi.org/10.1126/science.2432664
  37. Alexander CM, Selvarajan S, Mudgett J, Werb Z. Stromelysin-1 regulates adipogenesis during mammary gland involution. J Cell Biol 2001;152:693-703. https://doi.org/10.1083/jcb.152.4.693
  38. Gao D, Zhang X, Jiang X, Peng Y, Huang W, Cheng G, Song L. Resveratrol reduces the elevated level of MMP-9 induced by cerebral ischemia-reperfusion in mice. Life Sci 2006;78:2564-70. https://doi.org/10.1016/j.lfs.2005.10.030

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