The Journal of Korean Academy of Prosthodontics (대한치과보철학회지)

The Korean Academy of Prosthodonitics (대한치과보철학회)
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The effect of alendronates administration duration on the healing of extraction socket in rats: pilot study

알렌드로네이트의 투여기간이 발치와의 치유에 미치는 영향: 예비실험

  • Kim, Jee-Hwan (Department of Prosthodontics, College of Dentistry, Yonsei University) ;
  • Hong, Jong-Hwan (Department of Prosthodontics, College of Dentistry, Yonsei University) ;
  • Choi, Hyun-Min (Department of Prosthodontics, College of Dentistry, Yonsei University) ;
  • Park, Young-Bum (Department of Prosthodontics, College of Dentistry, Yonsei University) ;
  • Moon, Hong-Seok (Department of Prosthodontics, College of Dentistry, Yonsei University)
  • 김지환 (연세대학교 치과대학 보철학교실) ;
  • 홍종환 (연세대학교 치과대학 보철학교실) ;
  • 최현민 (연세대학교 치과대학 보철학교실) ;
  • 박영범 (연세대학교 치과대학 보철학교실) ;
  • 문홍석 (연세대학교 치과대학 보철학교실)
  • Received : 2013.06.13
  • Accepted : 2013.07.10
  • Published : 2013.07.31
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Abstract

Purpose: The purpose of this study was to investigate the effect of different administration duration of alendronate on initial wound healing and new bone formation of extraction socket in rats. Materials and methods: Fifteen male Sprague-Dawley rats (body weight 130-140 g, 4 weeks old, male) were divided into control group (no alendronate administration) and experimental group (alendronate administration). Experimental group was subdivided into 1 week administrated group, 2 week administrated group, 4 week administrated group and 6 week administrated group according to duration of administration. For the experimental groups, during the designated time period (at the time of extraction, 1 week before extraction, 3 week before extraction and 5 week before extraction) till 1 week after extraction, rats were subcutaneously injected with Alendronate at the dose of 1.0 mg/Kg three times a week. Each specimen from 6 week experimental group and control group were used for microarray analysis, and other specimens were used for histological analysis. The rate of new bone formation within the extraction site and bone loss activity was analyzed using TRAP staining. Statistical analysis was performed using Kruskal Wallis test. (${\alpha}=.05$) Results: After one week from the time of extraction, the rate of new bone formation within extraction site for the control group ($16.77%{\pm}1.36%$) compared to the 4 week experimental group ($14.99%{\pm}6.26%$) was lower. However, no statistically significant difference was found. Increase in the number of inactive lacuna (empty lacuna) and decrease in the number of TRAP positive cell were identified with increased duration of administration. There was no significant difference. Conclusion: The results of this study showed as the duration of Alendronate administration increased the rate of new bone formation decreased with loss of bone activity and reduced number of osteoclast.

연구 목적: 본 연구에서는 알렌드로네이트 투여기간의 차이가 백서의 상악 제 1대구치 발치와의 초기 창상치유에 미치는 영향을 알아보고자 하였다. 연구 재료 및 방법: 4주령 Sprague Dawley Rat (Body weight 130-140 g) 15마리를 이용하여 알렌드로네이트(Merial Inc., Parramatta, Australia) 투여군(실험군)과 투여하지 않은 대조군으로 나누고 실험군은 다시 투여기간에 따라 1주 투여군부터 2주 투여군, 4주 투여군, 6주 투여군까지 총 4개의 실험군으로 나눴다. 실험군에 정해진 시기(발치 5 주전, 발치 3주전, 발치 1주전, 발치 시)부터 발치 1주 후까지 알렌드로네이트를 1 mg/kg의 농도로 주 3회 피하주사 한 후 6주 투여군과 대조군에서 한 개체씩을 이용하여 미세배열(microarray)분석을 실시하였고, 다른 시편들을 이용하여 조직학적 분석을 시행하였다. 발치와 내에서의 신생골의 형성 비율과 발치와 주위 골에서 활성을 잃은 뼈에 대해 분석하였고, 파골세포 표지자인 TRAP 염색을 하여 분석하였다. 통계적인 분석으로 크루스컬-왈리스 검정(Kruskal Wallis test)을 실시하였다(${\alpha}=.05$). 결과:발치 후 1주 시점에서의 발치와 내에서의 신생골의 생성 비율은 대조군보다 ($16.77%{\pm}1.36%$) 4주 투여군($14.99%{\pm}6.26%$)에서 더 낮게 나오는 경향은 있었지만 유의 하지는 않았다. 투여기간이 증가할수록 활성을 잃은 골수강(empty lacuna)의 수는 증가하였고, TRAP positive cell 수는 감소하는 경향이 있으나 유의하지는 않았다. 결론: 본 예비실험을 통해서 알렌드로네이트의 투여기간이 증가할수록 신생골의 생성 비율이 감소되고 활성을 잃은 뼈들이 많아지며 파골세포의 수는 줄어드는 경향이 있음을 알 수 있었으나 통계적으로 유의차는 없었다.

Keywords

References

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