Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Application of Intracytoplasmic Sperm Injection in Mammals

  • Li, Xiao Xia (Department of Animal Science and Biotechnology, College of Agriculture Life Science, Chungnam National University) ;
  • Lee, Kyung Bon (Department of Animal Science and Biotechnology, College of Agriculture Life Science, Chungnam National University) ;
  • Lee, Ji Hye (Department of Animal Science and Biotechnology, College of Agriculture Life Science, Chungnam National University) ;
  • Kim, Keun Jung (Department of Animal Science and Biotechnology, College of Agriculture Life Science, Chungnam National University) ;
  • Park, Kang Sun (Department of Animal Science and Biotechnology, College of Agriculture Life Science, Chungnam National University) ;
  • Kim, Min Kyu (Department of Animal Science and Biotechnology, College of Agriculture Life Science, Chungnam National University)
  • Received : 2013.06.19
  • Accepted : 2013.06.24
  • Published : 2013.06.30
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Abstract

For more than two decades, the intracytoplasmic sperm injection (ICSI) technique has been used as a valuable tool to provide opportunities for studying fertilization, treating human infertility, and producing transgenic animals. Not only in facilitating fertilization but also in propagating mammalian species, ICSI has enhanced the potential of assisted reproductive technologies in human. Polyspermic fertilization has been one of major problems in pig reproduction, but the ICSI helped to solve the problem, and used widely to generate transgenic piglets. Although the ICSI technique is considered to be a very useful tool in assisted reproductive technologies, including generation of transgenic animals, there are some disadvantages using the technique. In this review, we describe the ICSI technique and its application in animal production and human infertility, and discuss advantage and disadvantage of the technique in mammals.

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References

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