Journal of Environmental Impact Assessment (환경영향평가)

Korean Society of Environmental Impact Assessment (한국환경영향평가학회)
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Study on potential environmental risk of virus resistant LM plants using co-inoculation of Zucchini green mottle mosaic virus (ZGMMV) and Cucumber mosaic virus (CMV)

ZGMMV와 CMV 동시 접종을 통한 바이러스 저항성 LM 식물의 잠재적 환경 위해성 연구

  • Song, Hae-Ryong (Biosafety Research Team, National Institute of Environmental Research (NIER)) ;
  • Kim, Taesung (Biosafety Research Team, National Institute of Environmental Research (NIER)) ;
  • Kim, Sun-Jung (Biosafety Research Team, National Institute of Environmental Research (NIER)) ;
  • Kim, Yong-Hyun (Biosafety Research Team, National Institute of Environmental Research (NIER)) ;
  • Kim, Ki-Jeong (Biosafety Research Team, National Institute of Environmental Research (NIER)) ;
  • Chung, Hyen-Mi (Biosafety Research Team, National Institute of Environmental Research (NIER)) ;
  • Choi, Hee Lak (Biosafety Research Team, National Institute of Environmental Research (NIER)) ;
  • Yoon, Junheon (Biosafety Research Team, National Institute of Environmental Research (NIER))
  • 송해룡 (국립환경과학원 바이오안전연구팀) ;
  • 김태성 (국립환경과학원 바이오안전연구팀) ;
  • 김선정 (국립환경과학원 바이오안전연구팀) ;
  • 김용현 (국립환경과학원 바이오안전연구팀) ;
  • 김기정 (국립환경과학원 바이오안전연구팀) ;
  • 정현미 (국립환경과학원 바이오안전연구팀) ;
  • 최희락 (국립환경과학원 바이오안전연구팀) ;
  • 윤준헌 (국립환경과학원 바이오안전연구팀)
  • Received : 2012.11.10
  • Accepted : 2013.02.01
  • Published : 2013.04.30
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Abstract

Plant virus coat (CP) gene-mediated protection is one of the best known approaches to protect against virus resistant transgenic plants. Transgenic N. benthamiana plants containing the CP gene of Zucchini green mottle mosaic virus (ZGMMV) were used for the environmental risk assessment of the living modified (LM) plants with plant virus resistance. The most optimal co-infection method of both ZGMMV and CMV (Cucumber mosaic virus) on Non-LM and CP-expressing LM tobacco plants was established and co-infection of CMV and ZGMMV was confirmed by polymerase chain reaction (PCR). To address the effects of LM tobacco plants on the mutation of the virus, in-vitro transcripts of CP and Replicase (Rep) derived from CMV and/or ZGMMV were inoculated onto Non-LM or LM tobacco plants. Mutation frequency of CP and Rep from CMV and ZGMMV was examined through six serial passages in Non-LM and LM tobacco plants. Little actual frequency of mutation was estimated, probably due to the limited number of transgenic plants tested in this study. However, it does not suggest environmental safety of these CP-mediated LM plants. Further study at a larger scale is needed to evaluate the environmental risk associated with the CP-expressing LM plants.

Keywords

References

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