한국섬유공학회지 (Textile Science and Engineering)

  • 제50권1호
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  • Pages.40-47
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  • 2013
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  • 1225-1089(pISSN)
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  • 2288-6419(eISSN)
한국섬유공학회 (The Korean Fiber Society)
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전기방사를 이용하여 제조한 PLGA/Graphene 복합 섬유지지체의 인장성질과 세포독성

Tensile Property and Cytotoxicity of Electrospun PLGA/Graphene Composite Scaffold

  • 강민지 (서울대학교 바이오시스템소재학부) ;
  • 김형환 (서울대학교 바이오시스템소재학부) ;
  • 박아름 (서울대학교 바이오시스템소재학부) ;
  • 김현정 (서울대학교 치과대학) ;
  • 박영환 (서울대학교 바이오시스템소재학부)
  • Kang, Min Ji (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Kim, Hyung Hwan (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Park, A Ruem (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Kim, Hyun Jung (Department of Dental Anesthesiology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Park, Young Hwan (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University)
  • 투고 : 2012.12.08
  • 심사 : 2013.01.30
  • 발행 : 2013.02.28
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, we investigated PLGA/graphene composite scaffold prepared by electrospinning method for its possible application in biomedical fields. Mixture of 16 wt% PLGA and 0.1~1.0 wt% well-dispersed graphene dissolved in 1,2-dichloroethane co-solvent was used as a dope solution for fabricating a mat type of electrospun PLGA/graphene composite scaffold. The morphological structure of the composite scaffold was examined by FE-SEM, indicating that monolayered graphene was well hybridized inside the PLGA microfiber (ca. 900~1000 nm fiber size). Indirect evidence of graphene inclusion in the composite scaffold was confirmed by X-ray diffraction analysis and thermal gravimetric analysis quantitatively. As a result of tensile test, the PLGA/graphene composite scaffold had an excellent tensile strength (2~2.5 times higher), breaking strain (2.5~3.5 times higher) and Young's modulus (1.2~2.4 times higher) compared to electrospun PLGA scaffold. Cytotoxicity and cell proliferation of the composite scaffold were also performed by MTT assay using MC3T3-E1 and NIH3T3 cell, showing that graphene-containing PLGA scaffold has no cytotoxicity and even much better cell adhesion and proliferation ability. Therefore, it is concluded that the electrospun PLGA/graphene composite scaffold can be potentially used in biomedical applications for tissue engineering, due to excellent mechanical properties as well as high cell compatibility.

키워드

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