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Tensile Property and Cytotoxicity of Electrospun PLGA/Graphene Composite Scaffold

전기방사를 이용하여 제조한 PLGA/Graphene 복합 섬유지지체의 인장성질과 세포독성

  • Kang, Min Ji (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Kim, Hyung Hwan (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Park, A Ruem (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Kim, Hyun Jung (Department of Dental Anesthesiology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Park, Young Hwan (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University)
  • 강민지 (서울대학교 바이오시스템소재학부) ;
  • 김형환 (서울대학교 바이오시스템소재학부) ;
  • 박아름 (서울대학교 바이오시스템소재학부) ;
  • 김현정 (서울대학교 치과대학) ;
  • 박영환 (서울대학교 바이오시스템소재학부)
  • Received : 2012.12.08
  • Accepted : 2013.01.30
  • Published : 2013.02.28

Abstract

In this study, we investigated PLGA/graphene composite scaffold prepared by electrospinning method for its possible application in biomedical fields. Mixture of 16 wt% PLGA and 0.1~1.0 wt% well-dispersed graphene dissolved in 1,2-dichloroethane co-solvent was used as a dope solution for fabricating a mat type of electrospun PLGA/graphene composite scaffold. The morphological structure of the composite scaffold was examined by FE-SEM, indicating that monolayered graphene was well hybridized inside the PLGA microfiber (ca. 900~1000 nm fiber size). Indirect evidence of graphene inclusion in the composite scaffold was confirmed by X-ray diffraction analysis and thermal gravimetric analysis quantitatively. As a result of tensile test, the PLGA/graphene composite scaffold had an excellent tensile strength (2~2.5 times higher), breaking strain (2.5~3.5 times higher) and Young's modulus (1.2~2.4 times higher) compared to electrospun PLGA scaffold. Cytotoxicity and cell proliferation of the composite scaffold were also performed by MTT assay using MC3T3-E1 and NIH3T3 cell, showing that graphene-containing PLGA scaffold has no cytotoxicity and even much better cell adhesion and proliferation ability. Therefore, it is concluded that the electrospun PLGA/graphene composite scaffold can be potentially used in biomedical applications for tissue engineering, due to excellent mechanical properties as well as high cell compatibility.

Keywords

References

  1. T. Kuilla, S. Bhadra, D. Yao, N. H. Kim, S. Bose, and J. H. Lee, "Recent Advances in Graphene Based Polymer Composites", Prog Polym Sci, 2010, 35, 1350-1375. https://doi.org/10.1016/j.progpolymsci.2010.07.005
  2. K. S. Kim, Y. Zhao, H. Jang, S. Y. Lee, J. M. Kim, K. S. Kim, J. H. Ahn, P. Kim, J. Y. Choi, and B. H. Hong, "Large-scale Pattern Growth of Graphene Films for Stretchable Transparent Electrodes", Nature, 2009, 457, 706-710. https://doi.org/10.1038/nature07719
  3. X. Lu, M. Yu, H. Huang, and R. S. Rouff, "Tailoring Graphite with the Goal of Achieving Single Sheets", Nanotechnology, 1990, 10, 269-272.
  4. C. D. Reddy, S. Rajendran, and K. M. Liew, "Equilibrium Configuration and Continuum Elastic Properties of Finite Sized Graphene", Nanotechnology, 2006, 17, 864-870. https://doi.org/10.1088/0957-4484/17/3/042
  5. T. R. Nayak, H. Andersen, V. S. Makam, C. Khaw, S. Bae, X. Xu, P. L. R. Ee, J. H. Ahn, B. H. Hong, G. Pastorin, and B. Ozyilmaz, "Graphene for Controlled and Accelerated Osteogenic Differentiation of Human Mesenchymal Stem Cells", ACSNano, 2011, 5(6), 4670-4678.
  6. N. Li, X. Zhang, Q. Song, R. Su, Q. Zhang, T. Kong, L. Liu, G. Jin, M. Tang, and G. Cheng, "The Promotion of Neurite Sprouting and Outgrowth of Mouse Hippocampal Cellsin Culture by Graphene Substrates", Biomaterials, 2011, 32, 9374-9382. https://doi.org/10.1016/j.biomaterials.2011.08.065
  7. Y. Liang, D. Wu, X. Feng, and K. Mullen, "Dispersion of Graphene Sheets in Organic Solvent Supported by Ionic Interactions", Adv Mater, 2009, 21, 1679-1683. https://doi.org/10.1002/adma.200803160
  8. B. W. Chieng, N. A. Ibrahim, W. Z. Yunus, M. Z. Hussein, and V. S. Silverajah, "Graphene Nanoplatelets as Novel Reinforcement Filler in Poly(lactic acid)/Epoxidized Palm Oil Green Nanocomposites: Mechanical Properties", Int J Mol Sci, 2012, 13, 10920-10934. https://doi.org/10.3390/ijms130910920
  9. Y. Zhang, S. F. Ali, E. Dervishi, Y. Xu, Z. Li, D. Casciano, and A. S. Biris, "Cytotoxicity Effects of Graphene and Single-Wall Carbon Nanotubes in Neural Phaeochromocytoma-Derived PC12 Cells", ACSNano, 2010, 4, 3181-3186.
  10. K. Wang, J. Ruan, H. Song, J. Zhang, Y. Wo, S. Guo, and D. Cui, "Biocompatibility of Graphene Oxide", Nanoscale Res Lett, 2011, 6, 2-8.
  11. D. Zhang, A. Tian, X. Xue, M. Wang, B. Qiu, and A. Wu, "The Effect of Temozolomide/Poly(lactide-co-glycolide) (PLGA)/Nano-Hydroxyapatite Microspheres on Glioma U87 Cells Behavior", Int J Mol Sci, 2012, 13, 1109-1125. https://doi.org/10.3390/ijms13011109
  12. D. Gray, A. McCaughan, and B. Mookerji, "Crystal Structure of Graphite, Graphene and Silicon", Lecture, Massachusetts Institute of Technology, Cambridge, MA, 2009.
  13. H. Chang and Y. Wang, "Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds", Regenerative Medicine and Tissue Engineering, 2011, 27, 569-588.