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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Production and Characterization of β-Glucan Type Oligomer Produced with Enzymatic Hydrolysis of Capsosiphon fulvescens

효소 가수분해를 통한 매생이 유래 β-Glucan 형태의 Oligomer 생산 및 분석

  • Kim, Hyun-Woo (Department of Chemical & Biochemical Engineering, Chosun University) ;
  • Lee, Jung-Heon (Department of Chemical & Biochemical Engineering, Chosun University)
  • 김현우 (조선대학교 생명화학공학과) ;
  • 이중헌 (조선대학교 생명화학공학과)
  • Received : 2013.03.26
  • Accepted : 2013.04.30
  • Published : 2013.06.27
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Abstract

${\beta}$-Glucan type oligomers which have angiotensin I converting enzyme (ACE) inhibitory activity were isolated and characterized from Capsosiphon fulvescens. After C. fulvescens was hydrolysis with Alcalase at $50^{\circ}C$, supernatant was harvested and separated with ultrafiltration membrane (MWCO 2 kDa). Oligomers which were less than 2 kDa of molecular weight were harvested for characterization. The nutrient composition of Alcalase hydrolysate was 89.9% carbohydrate, 4.2% protein and 5.9% sulfate. After ultrafiltration, the nutrient composition of oligomers was changed to 99.88% carbohydrate, 0.07% protein and 0.05% sulfate. The carbohydrate composition of oligomer was glucose (97.2%) and mannose (1.5%). The ACE inhibitory activities of Alcalase hydrolysate and oligomer were 72.1% and 82%, respectively. The molecular weight of oligomer was about 1 kDa. The oligomer was analyzed with FT-IR, $^1H$-NMR and methylation. The oligomers were ${\beta}$-1,3-glucans with ${\beta}$-(1,3)-linked glucose units.

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References

  1. Nagayama, K., Y. Iwamura, T. Shibata, I. Hirayama, and T. Nakamura (2002) Bactericidal activity of phlorotannins from the brown alga Ecklonia kurome. J. Antimicrob. Chemother. 50: 889-893. https://doi.org/10.1093/jac/dkf222
  2. Okai, Y., K. H. Okai, S. Ishizaka, K. Ohtani, I. M. Yuasa, and U. Yamashita (1998) Possible immunodulating activities in an extract of edible brown alga, Hijikia fusiforme (Hijiki). J. Sci. Food Agric. 76: 56-62. https://doi.org/10.1002/(SICI)1097-0010(199801)76:1<56::AID-JSFA927>3.0.CO;2-L
  3. Chen, C. Y. and H. N. Chou (2002) Screening of red algae filaments as a potential alternative source of eicosapentaenoic acid. Mar. Biotechnol. 4: 189-192. https://doi.org/10.1007/s10126-002-0002-4
  4. Frolich, L. and P. Riederer (1995) Free radical mechanisms in dementia of Alzheimer type and the potential for antioxidative treatment. Drug Res. 45: 443-449.
  5. Jung, K. J., C. H. Jung, J. H. Pyeun, and Y. J. Choi (2005) Changes of food components in Mesangi (Capsosiphon fulvecense), Gashiparae (Enteromorpha prolifera), and Cheonggak (Codium fragile) depending on harvest times. J. Korean Soc. Food Sci. Nutr. 34: 687-693. https://doi.org/10.3746/jkfn.2005.34.5.687
  6. Kwon, M. J. and T. J. Nam (2006) Effects of Mesangi (Capsosiphon fulvecens) powder on lipid metabolism in high cholesterol fed rats. J. Korean Soc. Food Sci. Nutr. 35: 530-535. https://doi.org/10.3746/jkfn.2006.35.5.530
  7. Jeong, K. S. and N. G. Lee (2010) A study on physiological activity and antioxidative activity of Maesangi (Capsosiphon fulvescens) extract. J. Environ. Sci. 19: 407-414. https://doi.org/10.5322/JES.2010.19.4.407
  8. Cho, M., I. J. Kang, M. H. Won, H. S. Lee, and S. You (2010) The antioxidant properties of ethanol extracts and their solvent-partitioned fractions from various green seaweeds. J. Med. Food. 13: 1232-1239. https://doi.org/10.1089/jmf.2010.1124
  9. Kim, M. H., Y. T. Chung, J. H. Lee, Y. S. Park, M. K. Shin, H. S. Kim, D. H. Kim, and H. Y. Lee (2000) Hepatic detoxification activity and reduction of serum alcohol concentration of Hovenia dulcis THUNB from Korea and China. Korean J. Medicinal Crop. Sci. 8: 225-233.
  10. Misuno, T., T. Hagiwara, T. Nakamura, H. Ito, K. Shimura, T. Sumiya, and A. Asakura (1990) Antitumor activity and some properties of water-soluble polysaccharides from "Himematsutake," the fruiting body of Agaricus blazei Murill. Agric. Biol. Chem. 54: 2889-2896. https://doi.org/10.1271/bbb1961.54.2889
  11. Hwang, Y. J., G. W. Noh, and S. H. Kim (2003) Effect of Inonotus obliquus extracts on proliferation and caspase-3 activity in human gastro-intestinal cancer cell lines. J. Korean Nutr. Soc. 36: 18-30.
  12. Komatsu, N., S. Okubo, S. Kikumoto, K. Kimura, G. Saito, and S. Sasaki (1969) Host mediated antitumor action of schizophyllan, a glucan produced by Schizophyllum commune. Gann. 60: 137-144.
  13. Kim, S. M., S. H. Kang, J. Y. Ma, and J. H. Kim (2006) A study on the extract and efficacy of bioactive compound from Hovenia dulcis. Korean J. Biotechnol. Bioeng. 21: 11-15.
  14. Kim, Y. M., I. H. Kim, and T. J. Nam (2011) Induction of apoptosis signaling by a glycoprotein of Capsosiphon fulvescens in AGS cell. Korean J. Fish Sci. 44: 216-224.
  15. Lee, J. H., Y. M. Lee, J. J. Lee, and M. Y. Lee (2006) Effects of Capsosiphon fulvescens extract on lipid metabolism in rats fed high cholesterol diet. J. Korean Soc. Food Sci. Nutr. 35: 402-409. https://doi.org/10.3746/jkfn.2006.35.4.402
  16. Heo, S. J., K. W. Lee, C. B. Song, and Y. J. Jeon (2003) Antioxidant activity of enzymatic extracts from brown seaweeds. Algae 18: 71-81. https://doi.org/10.4490/ALGAE.2003.18.1.071
  17. Dubois, M., K. A. Gills, J. K. Hamilton, P. A. Rebers, and F. Smith (1956) Colorimetric method for the determination of sugars and related substances. Anal. Chem. 28: 350-356. https://doi.org/10.1021/ac60111a017
  18. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  19. Dodgson, K. S. and R. G. Price (1962) A note on the determination of the ester sulfate content of sulfated polysaccharides. J. Biochem. 84: 106-110. https://doi.org/10.1042/bj0840106
  20. Lee, J. M., S. G. You, and S. M. Kim (2005) Functional activities of low molecular weight peptides purified from enzymatic hydrolysates of seaweeds. J. Korean Soc. Food Sci. Nutr. 34: 1124-1129. https://doi.org/10.3746/jkfn.2005.34.8.1124
  21. Kim, Y. M., J. R. Do, J. P. In, and J. H. Park (2005) Angiotensin I converting enzyme (ACE) inhibitory activities of laver (Porphyra tenera) protein hydrolysates. Korean J. Food Nutr. 18: 11-18.
  22. Kim, Y. M., Y. S. Choi, and J. H. Park (2006) Purification and chemical characterisation of Laminaran from Eisenia bicyclis in Korea. J. Korean Soc. Food Sci. Nutr. 35: 78-86. https://doi.org/10.3746/jkfn.2006.35.1.078
  23. Tredwell, L., J. A. Nicholas, L. R. Peter, and M. H. James (2003) NMR confirmation of an alkali-insoluble glucan from Kluyveromyces marxianus cultivated on a lactose-based medium. World J. Microbilogy Biotechnology. 19: 349-355. https://doi.org/10.1023/A:1023961411821
  24. Na, Y. S., W. J. Kim, S. M. Kim, J. K. Park, S. M. Lee, S. O. Kim, A. Synytsya, and Y. I. Park (2010) Purification, characterization and immunostimulating activity of water soluble polysaccharide isolated from Capsosiphon fulvescens. Int. Immunopharmacol. 10: 364-370. https://doi.org/10.1016/j.intimp.2009.12.011
  25. Zvyagintseva, T. N., N. M. Shevchenko, I. V. Nazarova, A. S. Scobum, P. A. Luk'yanov, L. A. Elyakova (2000) Inhibition of complement activation by water soluble polysaccharides of some fareastern brown seaweeds. Comp. Biochem. Physiol. C Pharmacol. Toxicol. Endocrinol. 126: 209-215. https://doi.org/10.1016/S0742-8413(00)00114-6
  26. Vlasta, M. G., B. Romano, and J. P. Gerwin (2001) Vibrational spectroscopic characterization of wild growing mushrooms and toadstools. Spectrochimica. Acta Part A 57: 2815-2829. https://doi.org/10.1016/S1386-1425(01)00584-4

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