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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Comparison of Volatile Compounds of Chamaecyparis obtusa Essential Oil and its Application on the Improvement of Atopic Dermatitis

추출방법에 따른 편백 정유의 향기 성분 비교 및 아토피 개선에의 응용

  • Lim, Geum-Sook (Interdisciplinary Program of Perfume and Cosmetics, Chonnam National University Graduate School) ;
  • Kim, Ran (Department of Herb Therapy & Cosmetics, Wonkwang Health Science University) ;
  • Cho, Hoon (Department of Polymer Science & Engineering, Chosun University) ;
  • Moon, Young-Sook (Research Center for Resistant Cells, Chosun University Medical School) ;
  • Choi, Chang-Nam (Interdisciplinary Program of Perfume and Cosmetics, Chonnam National University Graduate School)
  • 임금숙 (전남대학교 일반대학원 향장품학협동과정) ;
  • 김란 (원광보건대학교 허브테라피향장과) ;
  • 조훈 (조선대학교 공과대학 응용화학소재공학과) ;
  • 문영숙 (조선대학교 의과대학 내성세포연구센터) ;
  • 최창남 (전남대학교 일반대학원 향장품학협동과정)
  • Received : 2013.02.04
  • Accepted : 2013.03.28
  • Published : 2013.04.27
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Abstract

Volatile flavor compounds of Chamaecyparis obtuse essential oil were extracted by simultaneous steam distillation extraction (SDE) and supercritical fluid extraction (SFE) and analyzed by GC-MS. A total of 48 and 50 components were identified in essential oil by SDE and SFE, respectively. Monoterpenes, oxygenated monoterpenes, sesquiterpenes, oxygenated sesquiterpenes, and diterpenes in essential oil by SDE were 37.24, 10.9, 9.61, 0.22, and 0.22%, respectively. In the case of SFE, they were 19.1, 23.3, 22.66, 1.31, and 10.57%, respectively. Antioxidant activities were increased with the increase of essential oil up to $80{\mu}L/mL$, irrespective of extraction method. Especially, when the essential oil concentration extracted by SDE was increased from 20 to $80{\mu}L/mL$, the antioxidant activity was increased from 10.5 to 55.1%. However, over $80{\mu}L/mL$ of essential oil, an equilibrium state was maintained. In the case of essential oil extracted by SFE, it was decreased compared to that of SDE. For the improvement of atopic dermatitis, various cosmetics such as an ato-cide soap, ato-cide spray, and ato-cide lotion containing essential oil extracted by SFE were tested. About over 90% was useful for the improvement of atopic dermatitis after 4 weeks of clinical trial targeting 40 female adults. These results demonstrate that ato-cide soap, ato-cide spray, and ato-cide lotion containing essential oil extracted by SFE could be used in functional cosmetics.

Keywords

References

  1. Reineccius, G. A. (2007) Flavour-isolation techniques: Chemistry, Bioprocessing, and Sustainability. Berger RG (ed). pp. 409-414, Springer-Verlag, Berlin, Germany.
  2. Yun, K. S., J. H. Hong, and Y. H. Choi (2006) Characteristics of Elsholtzia splendens extracts on simultaneous steam distillation extraction conditions. Korean J. Food Preserv. 13: 623-628.
  3. Choi, Y. H., J. Kim, and K. P. Yoo (1999) Selective extraction of ephedrine from Ephedra sinica using mixture of $CO_{2}$ diethylamine and methanol. Chromatographia. 50: 673-679. https://doi.org/10.1007/BF02497302
  4. Choi, Y. H., J. H. Ryu, K. P. Yoo, Y. S. Chang, and J. Kim (1989) Supercritical carbon dioxide extraction of podophyllotoxin from Dysosma pleinatha roots. Planta Med. 64: 482-483.
  5. Lucien, F. P. and N. R. Foster (2000) Solubilities of solid mixtures in supercritical carbon dioxide: A review. J. Supercrit. Fluid. 17: 111-134. https://doi.org/10.1016/S0896-8446(99)00048-0
  6. Hubert, P. and O. G. Vitzthum (1978) Fluid extraction of hop, spices, and tobacco with supercritical gases. Angew. Chem. Int. Edit. 17: 710-715. https://doi.org/10.1002/anie.197807101
  7. Mishra, A. K. and N. K. Dubey (1994) Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities. Appl Environ Microbiol . 60(4):1101-1105.
  8. Price, S., L. Price, and S. Pe'noel D (1995) Aromatherapy for Health professionals. 1st ed., pp. 133-167. Chrchill Livingstone, New York.
  9. Hayashi, S., K. Yano, and T. Matsuura (1964) The monoterpene constituents of the essential oil from hinoki (Chamaecyparis obtusa). Bull.Chem.Soc.Japan. 37: 608-683.
  10. Kim, Y. Y. (2006) Health and phytoncide of forest, Res. J. Cheju National Univ. 35: 302-307.
  11. Lin, T. C., J. M. Fang, and Y. S. Cheng (1999) Terpenes and lignans from leaves from Chamaecyparis formosensis. Phytochem. 51: 793-801. https://doi.org/10.1016/S0031-9422(99)00074-6
  12. Hong, C. U., C. S. Kim, N. G. Kim, and Y. H. Kim (2001) Composition of essential oils from the leavesand the fruits of Chamaecyparis obtusa and Chamaecyparis pisifera. Korean Soc. Agric. Chem. Biotechnol. 44: 116-121.
  13. Jung, J. Y., J. W. Kim, Y. S. Kim, H. M. Park, B. H. Lee, M. S. Choi, and J. K. Yang (2012) Antifungal activity of extracts from Chamaecyparis obtusa and Pseudotsuga menziesii against Trichoderma spp. J. Agr. Life Sci. 45(4): 1-11.
  14. Chang, M. J. and S. R. Lee (2009) A comparative study on the compositions of Hwangryeonhaedok-tang's essential oils obtained by supercritical carbon dioxide extraction and hydrodistillation methods. J. Meridian Acupoint. 26: 211-223.
  15. Eskin, N. A. M. (1979) Terpenoids and flavonoids: Flavor and textures: The chemistry and biochemistry of selexted compounds, pp. 69-93. Academic press, New York, U.S.A.
  16. Nykanen, I., L. Nykanen, and M. Alkio (1991) Composition of angelica root oils obtained by supercritical $CO_{2}$ extraction and steam distillation. J. Ess.Oil Rs. 3: 229-236. https://doi.org/10.1080/10412905.1991.9697933
  17. Hayashi, S., K. Yano, and T. Matsuura (1964) The monoterpene consituents of the essential oil from hinoki (Chamaecyparis obtusa). Bull. Chem, Soc. Japan 37: 474-476. https://doi.org/10.1246/bcsj.37.474
  18. Shieh, B., Y. Iizuka, and Y. Matsubara (1981) Monoterpenenoid and sesquiterpenoid consituents of the essential oil of hinoki (Chamaecyparis obtuse). Agric. Biol. Chem. 45: 1497-1499. https://doi.org/10.1271/bbb1961.45.1497
  19. Carson, C. F. and T. V. Riley (1995) Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. J. Appli. Bacteriol. 78: 264-269. https://doi.org/10.1111/j.1365-2672.1995.tb05025.x
  20. Singh, P., R. Shukla, B. Prakash, A. Kumar, S. Singh, P. K. Mishra, and N. K. Dubey (2010) Chemical profile, antifungal, antiaflatoxigenic and antioxidant activity of Citrus maxima Burm. and Citrus sinensis (L.) Osbeck essential oils and their cyclic monoterpene, DL-limonene. Food Chem. Toxicol. 48: 1734-1740. https://doi.org/10.1016/j.fct.2010.04.001
  21. Rozza, A. L., D. M. Moraes, T., Kushima, H., Tanimoto, A., Mayo Marques, M. O., Bauab, T. M., Hiruma-Lima, and C. H. Pellizzon (2010) Ariane Leite Rozza, Thiago de Mello Moraes, Helio Kushima, Alexandre Tanimoto, Marcia Ortiz Mayo Marques, Tais Maria Bauab, Clelia Akiko Hiruma-Lima, Claudia Helena Pellizzon. 6: 1380-1387.
  22. Dorman, H. J., M. Kosar, K. Kahlos, Y. Holm, and R. Hiltunen (2003) Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars. J. Agr. Food Chem. 51: 4563-4569. https://doi.org/10.1021/jf034108k
  23. Decker, E. A. (1997) Phenolics: prooxidants or antioxidants. Nutrition Review. 55: 396-407.
  24. Germann. (2005) Terpenoids as plant antioxidants, Vitamins Hormones. 72: 505-535. https://doi.org/10.1016/S0083-6729(05)72015-X
  25. Choi, H. S., H. S. Song, H. Ukeda, and M. Sawamura (2000) Radical-scavenging activities of citruss essential oils and their components. J. Agr. Food Chem. 48: 4156-4161. https://doi.org/10.1021/jf000227d
  26. Gwak, K. S., M. J. Park, E. B. Jeung, J. W. Chang, and I. G. Choi (2006) Comparison of antifungal activities of monoterpenes and sesquiterpenes in essential oil from Chamaecyparis obtuse against dermatophytes. Mokchae Konghak. 34: 46-55.

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