Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Phenolic Content, DPPH Radical Scavenging, and Tyrosinase Inhibitory Activities of Ecklonia cava Extracted with the Ultrasonic Wave Method

초음파 추출법에 의한 감태 추출물의 페놀성 화합물 함량, DPPH 라디칼 소거 활성 및 tyrosinase 저해 활성 분석

  • Kim, So Jung (Kyeongbuk Institute for Marine Bio-Industry) ;
  • Kim, Donggiun (Department of Biological Science, Silla University) ;
  • Park, Jongbum (Department of Biological Science, Silla University) ;
  • Lee, Taek-Kyun (South Sea Environment Research Department, Korea Institute of Ocean Science and Technology)
  • 김소정 (경북해양바이오산업연구원) ;
  • 김동균 (신라대학교 생명과학과) ;
  • 박종범 (신라대학교 생명과학과) ;
  • 이택견 (한국해양과학기술원 남해특성연구부)
  • Received : 2013.06.19
  • Accepted : 2013.07.12
  • Published : 2013.07.30
Download PDF
⟨ Previous Next ⟩

Abstract

The applicability of the ultrasonic wave method to the extraction of useful components from seaweeds was investigated. Extracts from freeze-dried Ecklonia cava powder were prepared with hot water ($65^{\circ}C$), water ($24^{\circ}C$), 50% ethanol, and 100% ethanol, and ultrasonic extraction was also performed. The content of phenolic compounds and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and tyrosinase inhibitory activity of the extracts were analyzed, and differences in the data obtained by the ultrasonic extraction and the traditional extraction methods were compared. The phenolic content in the E. cava extract by ultrasonic extraction (142.80 mg/g) was approximately 14 times higher than the phenolic content in the hot water extract (10.03 mg/g). The DPPH radical scavenging and the tyrosinase inhibitory activities of the ultrasonic extract were approximately 4 times and 14 times higher than the hot water extracts, respectively. The correlation between the phenolic content and the DPPH radical scavenging activity ($R^2$=99.47) and between the phenolic content and the tyrosinase inhibitory activity ($R^2$=99.99) was very high. These results indicate that ultrasonic extraction is more suitable than traditional extraction for the extraction of useful components from E. cava.

해조류 유래 유용성분 추출을 위한 초음파 추출법의 적용성을 검토하였다. 동결건조한 감태 분말로부터 열수($65^{\circ}C$), 물($24^{\circ}C$), 50% 에탄올 및 100% 에탄올 뿐만 아니라 초음파 추출을 수행하여, 추출물을 제조하였다. 추출물의 페놀성 화합물 함량, DPPH 라디칼 소거 활성 및 tyrosinase 저해 활성을 분석하고, 초음파 추출과 전통적인 추출법의 차이를 비교하였다. 초음파 추출에 의한 감태 추출물의 페놀성 화합물의 함량(142.80 mg/g)은 열수 추출물의 페놀성 화합물 함량(10.03 mg/g)의 약 14배 이상 높았다. 열수 추출물에 비해 초음파 추출물의 DPPH 라디칼 소거 활성은 약 4배, tyrosinase 저해 활성은 약 14배 이상 높은 것으로 나타났다. 특히 초음파 추출법에 의한 추출물에서의 페놀성 화합물 함량과 DPPH 라디칼 소거활성 및 페놀성 화합물 함량과 tyrosinase 저해 활성간의 상관관계가 매우 높은 것(각각 $R^2$=99.47 및 99.99)으로 나타났다. 이상의 결과는 전통적인 용매 추출법보다 초음파 추출법이 감태의 유용성분 추출에 더 적합함을 의미한다.

Keywords

References

  1. Cha, S. H., Ahn, G. N., Heo, S. J., Kim, K. N., Lee, K. W., Song, C. B., Cho, S. K. and Jeon, Y. J. 2006. Screening of extracts from marine green and brown algae in Jeju for potential marine angiotensin-1 converting enzyme (ACE) inhibitory activity. J Korean Soc Food Sci Nutr 35, 307-317. https://doi.org/10.3746/jkfn.2006.35.3.307
  2. Chung, K. W., Kim, W. I., Hong, I. K. and Park, K. A. 2000. Ultrasonic energy effects on squalene extraction from amaranth seed. Appl Chem 4, 149-152.
  3. Cushnie, T. T. P. and Lamb, A. J. 2005. Antimicrobial activity of flavonoids. Int J Antimicrob Agents 26, 343-356. https://doi.org/10.1016/j.ijantimicag.2005.09.002
  4. Devi, G. K., Manivannan, K., Thirumaran, G., Rajathi, F. A. A. and Anantharaman, P. 2011. In vitro antioxidant activities of selected seaweeds from Southeast coast of India. Asian Pac J Trop Med 4, 205-211. https://doi.org/10.1016/S1995-7645(11)60070-9
  5. Fujimura, T., Tsukahara, K., Moriwaki, S., Kitahara, T., Sano, T. and Takema, Y. 2002. Treatment of human skin with an extract of Fucus vesiculosus changes its thickness and mechanical properties. J Cosmet Sci 53, 1-9.
  6. Ibanez, E., Herrero, M., Mendiola, J. A. and Castro-Puyana, M. 2012. Extraction and characterization of bioactive compounds with health benefits from marine resources: Macro and micro algae, cyanobacteria, and invertebrates. pp. 55-98. In: M. Hayes (ed.), Marine bioactive compounds: sources, characterization and applications. Springer Science+Business Media.
  7. Kim, D. H., Kim, H. J. and Chung, B. W. 2006. Extraction of anti-oxidative substance from Haematococcus pluvialis using ultrasonification. J Eng Res 37, 79-86.
  8. Kim, J. H., Kim, D. H., You, J. H., Kim, C. H., Kwon, M. C., Seong, N. S., Lee, S. E. and Lee, H. Y. 2005. Immune-regulatory activities of various fractions from Ehpedrae Sinica Stapf, Rubus Coreanus Miq. and Angelica gigas Nakai extracts with ultrasonification. Korean J Med Crop Sci 13, 161-170.
  9. Kim, J. K., Cha, W. S., Park, J. H. and Oh, S. L. 1997. Inhibition effect against tyrosinase of condensed tannins from Korea green tea. Korean J Food Sci Technol 29, 173-177.
  10. Kim, K. N., Lee, K. W., Song, C. B. and Jeon, Y. J. 2006. Cytotoxic activities of green and brown seaweeds collected from Jeju island against four tumor cell lines. J Food Sci Nutr 11, 17-24. https://doi.org/10.3746/jfn.2006.11.1.017
  11. Kim, S., Woo, S., Yun, H., Yum, S., Choi, E., Do, J.-R., Jo, J.-H., Kim, D., Lee, S. and Lee, T.-K. 2005. Total Phenolic Contents and Biological Activities of Korean Seaweed Extracts. Food Sci Biotechnol 14, 798-802.
  12. Kim, Y. S. and Choi, J. M. 2007. Effect of ultrasonic treatment on colorants extraction dyeability of safflower. J Korean Home Econ Assoc 45, 13-20.
  13. Knorr, D., Zenker, M., Heinz, V. and Lee, D. U. 2004. Application and potential of ultrasonics in food processing. Trends Food Sci Technol 15, 261-266. https://doi.org/10.1016/j.tifs.2003.12.001
  14. Kumar, C. S., Ganesan, P., Suresh, P. V. and Bhaskar, N. 2008. Seaweeds as a source of nutritionally beneficial compounds-a review. J Food Sci Technol 45, 1-13.
  15. Kumar, M., Gupta, V., Kumari, P., Reddy, C. R. K. and Jha, B. 2011. Assessment of nutrient composition and antioxidant potential of Caulerpaceae seaweeds. J Food Comp Anal 24, 270-278. https://doi.org/10.1016/j.jfca.2010.07.007
  16. Kwon, K. N., Choi, H. S. and Cha, B. S. 2009. Effects of microwave and ultrasonication on chitin extraction time. Korean J Food Nutr 22, 8-13.
  17. Lee, K. J. and Row, K. H. 2006. Enhanced extraction of isoflavones from Korean soybean by ultrasonic wave. Korean J Chem Eng 23, 779-783. https://doi.org/10.1007/BF02705927
  18. Lee, S. H., Kim, K. N., Cha, S. H., Ahn, G. N. and Jeon, Y. J. 2006. Comparison of antioxidant activities of enzymatic and methanolic extracts from Ecklonia cava stem and leave. J Korean Sci Food Sci Nutr 35, 1139-1146. https://doi.org/10.3746/jkfn.2006.35.9.1139
  19. Lu, Y. and Foo, L. Y. 2000. Antioxidant and radical scavenging activities of polyphenols apple pomace. Food Chem 68, 81-85. https://doi.org/10.1016/S0308-8146(99)00167-3
  20. Meenakshi, S., Umayaparvathi, S., Arumugam, M. and Balasubramanian, T. 2012. In vitro antioxidant properties and FTIR analysis of two seaweeds of Gulf of Mannar. Asian Pac J Trop Med S66-S70.
  21. Paniwnyk, L., Beaufoy, E., Lorimer, J. P. and Mason, T. J. 2001. The extraction of rutin from flower buds of Sophora japonica. Ultrason Sonochem 8, 299-301. https://doi.org/10.1016/S1350-4177(00)00075-4
  22. Park, J. H., Lee, H. S., Mun, H. C., Kim, D. H., Seong, N. S., Jung, H. G., Bang, J. K. and Lee, H. Y. 2004. Improvement of Anticancer Activation of Ultrasonificated Extracts from Acanthopanax senticosus Harms, Ephedra sinica Stapf, Rubus coreanus Miq. and Artemisia capillaris Thunb. Korean J Med Crop Sci 12, 273-278.
  23. Scheuer, P. J. 1990. Bioorganic marine chemistry. pp. 123. Springer Verlag.
  24. Singh, N. and Rajini, P. S. 2004. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 85, 611-616. https://doi.org/10.1016/j.foodchem.2003.07.003
  25. Woo, J. H., Shin, S. L., Chang, W. D. and Lee, C. H. 2009. Comparison of antioxidant effects by different extraction methods in flowers of Aster scaber, Aster maackii, Coreopsis lanceolata and Coreopsis tinctoria. Korean J Plant Res 22, 381-388.
  26. Xia, T., Shi, S. and Wan, X. 2006. Impact of ultrasonic assisted extraction on the chemical and sensory quality of tea infusion. J Food Eng 74, 557-560. https://doi.org/10.1016/j.jfoodeng.2005.03.043
  27. Ye, H., Wang, K., Zhou, C., Liu, J. and Zeng, X. 2008. Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum. Food Chem 111, 428-432. https://doi.org/10.1016/j.foodchem.2008.04.012
  28. Yu, S. H., Chong, M. S., Kim, H. J. and Lee, K. N. 2007. Studies on the extraction method and polysaccharide of tricholoma matsutake using the supersonic wave and microwave. Korean J Orient Physiol Pathol 21, 1431-1436.

Cited by

  1. A Study on Functional components, antioxidant activity of Gastrodiae Rhizoma and Gastrodiae Elata floral axis vol.30, pp.1, 2015, https://doi.org/10.6116/kjh.2015.30.1.19.
  2. Enhancement of Cognitive Functions byAronia melanocarpaElliot Through an Intermittent Ultrasonication Extraction Process vol.19, pp.3, 2016, https://doi.org/10.1089/jmf.2015.3519