Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Phylogenetic Relationships of the Genus Hemerocallis in Korea using rps16-trnK Sequences in Chloroplast DNA

엽록체 rps16-trnK 서열에 의한 한국 내 원추리속 식물종의 계통 관계

  • 허만규 (동의대학교 자연과학대학 분자생물학과) ;
  • 권오성 (동의대학교 자연과학대학 분자생물학과) ;
  • 이병룡 (서원대학교 생물교육과)
  • Received : 2013.01.24
  • Accepted : 2013.06.28
  • Published : 2013.07.30
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Abstract

The genus Hemerocallis (family Xanthorthoeaceae) is a herbaceous species, some of which are very important in herbal medicines. We evaluated the rps16-trnK region of the chloroplast DNA of a representative sample of eight taxa in Korea to estimate phylogenetic relationships within the taxa of this genus. Due to differences in the number of inserted nucleotides, the aligned data for Hemerocallis ranged from 729 (H. aurantiaca) to 742 nucleotides (H. fulva var. kwanso), with a mean of 736. Although several small indels and 20 inserts were present, sequence variation within the Hemerocallis genus was mostly due to nucleotide substitutions. All rps16-trnK trees generated in Korea exhibited a well-solved topology, with high bootstrap support, irrespective of the methods (parsimony) and the setting used. The node of H. minor and H. littorea was strongly supported, with a high bootstrap value in three trees, and these two taxa were sistered with H. thunbergii. The number of chromosomes was not congruent with that found in a previous study with RAPD, but the number was in agreement with the results of this study.

원추리속 식물은 초본류이며 이 속의 일부 종은 약용으로 중요하다. 이 속의 8개 분류군에 대해 엽록체의 rps16-trnK 부위로 계통관계를 평가하였다. 배당된 서열은 원추리(H. aurantiaca)에서 729 핵산 수로 가장 적었으며 왕원추리(H. fulva var. kwanso)에서 742 핵산 수로 가장 많았다. 그 차이는 염기 삽입에 기인하였다. 비록 일부 인델(indel)과 20개 염기의 삽입이 발견되었지만 서열 내 변이는 염기 치환이 많았다. rps16 - trnK에 의한 한국내 원추리속 분류군들은 높은 지지도로 잘 분리되었다. 애기원추리(H. minor)와 홍도원추리(H. littorea)는 높은 지지도로 같은 분지군을 형성하였으며 이 분지군은 노랑원원추리와 자매군을 형성하였다. 염색체의 수는 기존 보고된 RAPD의 결과와는 일치하지 않으나 본 연구와는 일치하였다.

Keywords

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