DOI QR코드

DOI QR Code

Mycoflora of Soybeans Used for Meju Fermentation

  • Kim, Dae-Ho (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Seon-Hwa (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kwon, Soon-Wo (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Jong-Kyu (Tree Pathology and Mycology Laboratory, Division of Forest Environment Protection, Kangwon National University) ;
  • Hong, Seung-Beom (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration)
  • Received : 2013.03.20
  • Accepted : 2013.05.02
  • Published : 2013.06.30

Abstract

Diverse fungi are present in Korean traditional meju and they are known to play an important role in fermented soybean products. To determine the origin of the fungi in meju, we examined the mycoflora of soybeans from 10 traditional meju factories. The samples were untreated or treated with sodium hypochlorite, and placed on malt extract agar (MEA), dichloran 18% glycerol agar (DG18), and dichloran rose bengal chloramphenicol agar (DRBC) medium. A total of 794 fungal strains were isolated and they were identified as 41 genera and 86 species. From sodium hypochlorite untreated soybeans, the genera, Cladosporium (55%), Eurotium (51%), Fusarium (33%), Penicillium (22%), and Aspergillus (exclusion of Eurotium) (20%), were mainly isolated, and Eurotium herbariorum (22%), Eurotium repens (18%), Cladosporium tenuissimum (18%), F. fujikuroi (18%), Aspergillus oryzae/flavus (7%), and Penicillium steckii (6%) were the predominant species. In case of sodium hypochloritetreated soybeans, Eurotium (31%) and Cladosporium (5%) were frequently isolated, but Aspergillus (excluding Eurotium), Penicillium and Fusarium which were frequently isolated from untreated soybeans, were rarely isolated. Eurotium herbariorum (21%), Eurotium repens (8%), and Cladosporium tenuissimum (3%) were the predominant species. Of the 41 genera and 86 species isolated from soybeans, 13 genera and 33 species were also found in meju. These results suggest that the fungi on soybeans may influence the mycoflora of meju.

Keywords

References

  1. Ann YG. Jang (fermented soybean) in official and royal documents in Chosun dynasty period. Korean J Food Nutr 2012;25:368-82. https://doi.org/10.9799/ksfan.2012.25.2.368
  2. Kim GY, Moon HK, Lee SW, Moon JN, Yoon WJ. Effect of mixed soybeans materials on quality characteristics of traditional soybean paste (Doenjang) during aging. Korean J Food Cookery Sci 2010;26:314-22.
  3. Yoo KM, Hwang JY, Lee SM. Development of soybean snacks and analysis of bioactive compounds. Korean J Food Nutr 2011;24:702-7. https://doi.org/10.9799/ksfan.2011.24.4.702
  4. Kim DH, Lee M, Baek SY, Lee JK, Samson RA, Hong SB. Identification and extracellular enzyme activities of Penicillium strains isolated from meju. J Microbiol 2013 (in press).
  5. Hong SB, Kim DH, Lee M, Baek SY, Kwon SW, Samson RA. Taxonomy of Eurotium species isolated from meju. J Microbiol 2011;49:669-74. https://doi.org/10.1007/s12275-011-0376-y
  6. Hong SB, Kim DH, Lee M, Baek SY, Kwon SW, Houbraken J, Samson RA. Zygomycota associated with traditional meju, a fermented soybean starting material for soy sauce and soybean paste. J Microbiol 2012;50:386-93. https://doi.org/10.1007/s12275-012-1437-6
  7. Lee SS. Meju fermentation for a raw material of Korean traditional soy products. Kor J Mycol 1995;23:161-75.
  8. Yum KJ, Park EU. Occurrence and distribution of soybean seed-borne fungi in Korea. Korean J Plant Pathol 1989;5:287-93.
  9. Samson RA, Hoekstra ES, Lund F, Filtenborg O, Frisvad JC. Methods for the detection, isolation and characterization of food-borne fungi. In: Samson RA, Hoekstra ES, Frisvad JC, editors. Introduction to food- and airborne fungi. 7th ed. Utrecht: Centraalbureau voor Schimmelcultures; 2004. p. 283-97.
  10. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved gens from filamentous ascomycetes. Appl Environ Microbiol 1995; 61:1323-30.
  11. Bensch K, Groenewald JZ, Dijksterhuis J, Starink-Willemse M, Andersen B, Summerell BA, Shin HD, Dugan FM, Schroers HJ, Braun U, et al. Species and ecological diversity within the Cladosporium cladosporioides complex (Davidiellaceae, Capnodiales). Stud Mycol 2010;67:1-94. https://doi.org/10.3114/sim.2010.67.01
  12. Geiser DM, Jiménez-Gasco MM, Kang S, Makalowska I, Veeraraghavan N, Ward TJ, Zhang N, Kuldau GA, O'Donnell K. FUSARIUM-ID v. 1.0: a DNA sequence database for identifying Fusarium. Eur J Plant Pathol 2004;110:473-9. https://doi.org/10.1023/B:EJPP.0000032386.75915.a0
  13. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungus ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
  14. Samson RA, Hoekstra ES, Frisvad JC. Identification of the common food- and airborne fungi. In: Samson RA, Hoekstra ES, Frisvad JC, editors. Introduction to food- and airborne fungi. 7th ed. Utrecht: Centraalbureau voor Schimmelcultures; 2004. p. 1-282.
  15. Pitt JI, Hocking AD. Fungi and food spoilage. 3rd ed. New York: Springer; 2009. p. 53-353.
  16. Pitt JI, Hocking AD. Fungi and food spoilage. 3rd ed. New York: Springer; 2009. p. 19-51.
  17. Cho WD, Shin HD. List of plant diseases in Korea. 4th ed. Suwon: Korean Society of Plant Pathology; 2004. p. 55-9.
  18. Li S, Hartman GL, Boykin DL. Aggressiveness of Phomopsis longicolla and other Phomopsis spp. on soybean. Plant Dis 2010;94:1035-40. https://doi.org/10.1094/PDIS-94-8-1035
  19. Seaman WL, Shoemaker RA, Peterson EA. Pathogenicity of Corynespora cassiicola on soybean. Can J Bot 1965;43:1461-9. https://doi.org/10.1139/b65-154
  20. Pitt JI, Hocking AD, Bhudhasamai K, Miscamble BF, Wheeler KA, Tanboon-Ek P. The normal mycoflora of commodities from Thailand. 2. Beans, rice, small grains and other commodities. Int J Food Microbiol 1994;23:35-53. https://doi.org/10.1016/0168-1605(94)90220-8