한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제26권3호
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  • Pages.383-388
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  • 2013
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
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Plant Regeneration from Anther Culture of Panax ginseng

  • Lee, Hee-Young (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Khorolragchaa, Altanzul (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Sun, Myung-Suk (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Kim, Young-Joon (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Kim, Yu-Jin (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Kwon, Woo-Seang (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Yang, Deok-Chun (Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
  • 투고 : 2013.06.05
  • 심사 : 2013.06.21
  • 발행 : 2013.06.30
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초록

The research concerned of the regeneration of plants from embryos obtained from anther cultures of ginseng (Panax ginseng C. A. Meyer). The aim was to determine the influence of the regeneration medium on the efficiency of the regeneration process. We conducted to determine the optimum conditions such as cold pretreatment, plant growth regulators and carbon sources on anther culture of P. ginseng. Highest callus formation rate was obtained when flower buds pretreated at $4^{\circ}C$ for 1 day. Among the treated growth regulators with various degrees of concentration in Murashige and Skoog's (MS) medium, 4.53 ${\mu}M$ of 2.4-dichlorophenoxyacetic acid and 4.44 ${\mu}M$ of 6-benzylaminopurine gives the most responsive callus with the frequency of 73.89% and 129.53 g of fresh weight. When we used 3-9% of sucrose and maltose among the different kinds and various concentrations of carbohydrates, callus was formed highest 67.29% in the medium with 3% of sucrose. Shoots induced from callus supplemented with 28.9 ${\mu}M$ of gibberellic acid and rooted in Gamborg's B5 medium supplemented with 14.7 ${\mu}M$ of indole-3-butyric acid.

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참고문헌

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피인용 문헌

  1. Biotechnological Interventions for Ginsenosides Production vol.10, pp.4, 2020, https://doi.org/10.3390/biom10040538