The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Protective Effects of Ukgan-san in $CoCl_2$-induced Cell Death of C6 Glial Cells

$CoCl_2$로 유도된 C6 신경교세포의 사멸에 대한 억간산(抑肝散)의 보호 효과

  • Cho, Mun-Young (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Shin, Yong-Jeen (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Ha, Ye-Jin (Traditional korean medicine research support team, Korea health industry development institute) ;
  • Woo, Chan (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Kim, Ta-Jung (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • You, Ju-Yeon (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Choi, Yong-Seok (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Choi, Jung-Hoon (Hamsoa Oriental Medical Clinic) ;
  • Shin, Sun-Ho (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University)
  • 조문영 (원광대학교 한의과대학 내과학교실) ;
  • 신용진 (원광대학교 한의과대학 내과학교실) ;
  • 하예진 (한국보건산업진흥원 한의약연구지원팀) ;
  • 우찬 (원광대학교 한의과대학 내과학교실) ;
  • 김태정 (원광대학교 한의과대학 내과학교실) ;
  • 유주연 (원광대학교 한의과대학 내과학교실) ;
  • 최용석 (원광대학교 한의과대학 내과학교실) ;
  • 최정훈 (함소아 한의원) ;
  • 신선호 (원광대학교 한의과대학 내과학교실)
  • Published : 2013.06.30
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Abstract

Objectives : In this study, we made an effort to investigate the protective mechanism of Ukgan-san (UGS) extracts on hypoxia-induced C6 glial cell death. Methods : The cell viability was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MMT) assay and cell morphological changes were analysed with microscope after staining with crystal violet (CV). Reactive oxygen species (ROS) formation was assessed by flow cytometer after staining with 2'7'-dichlorofluorescein diacetate (DCF-DA). We also analyzed expression of hypoxia-inducible factor-1 alpha (HIF-$1{\alpha}$) and p53, processing of procaspase-3 and procyclic acidic repetitive protein (PARP) by western blot method. Results : We estimated the elevated cell viability by UGS extract on $CoCl_2$-induced C6 glial cells. UGS attenuated $CoCl_2$-induced ROS formation in C6 glial cells and also showed a protective activity compared to antioxidants and exhibited abrogation of LDH-released by $CoCl_2$. UGS suppressed the typical apoptotic cell death markers, caspase-3 and PARP activation. UGS inhibited $CoCl_2$-induced HIF-1${\alpha}$ expression which is known as a major regulator for hypoxia-induced cell death, and suppressed p53 expression. Conclusions : These results suggest that UGS extract contains protective constituents for hypoxia-induced C6 glial cell death.

Keywords

References

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