Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Apoptotic Effect of Phellodendri Cortex Water Extract on MIA PaCa-2 Cells

췌장암 세포주 MIA PaCa-2에서 황백 물 추출물에 의한 Apoptosis 유도 및 작용기전

  • Lee, In Young (Department of Internal Medicine, College of Korean Medicine) ;
  • Jeong, Hwang San (Department of Internal Medicine, College of Korean Medicine) ;
  • Won, Jin Hee (Department of Internal Medicine, College of Korean Medicine)
  • 이인영 (원광대학교 한의과대학 내과학교실) ;
  • 정황산 (원광대학교 한의과대학 내과학교실) ;
  • 원진희 (원광대학교 한의과대학 내과학교실)
  • Received : 2013.03.06
  • Accepted : 2013.04.11
  • Published : 2013.04.25
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Abstract

The purpose of this study is to investigate the apoptotic effect of Phellodendri Cortex water extract (PCWE) on pancreatic cancer cells and to find out the regulating mechanisms. Human-derived pancreatic cancer cell line, MIA PaCa-2 cells were treated by PCWE with various concentrations and the cytotoxicity was determined by MTT assay. The activation of Annexin V, DNA fragmentation, cell cycle arrest and caspase activation were observed to investigate the role of PCWE in pancreatic cancer cells. Also, to find out the regulating mechanisms, we examined the ROS production. The treatment of PCWE induced the cell death in both concentration and time dependent manner. The treatment of PCWE also increased the expression of Annexin V, DNA fragmentation, cell cycle arrest, and cleavage of caspase, which means cell-death PCWE induced was apoptosis but not necrosis. The ROS production was increased by PCWE treatment and the blockade of ROS inhibited the PCWE-induced cell death. These results could suggest that PCWE induced apoptosis via ROS release in pancreatic cancer cell.

Keywords

References

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