생명과학회지 (Journal of Life Science)

  • 제23권6호
  • /
  • Pages.838-846
  • /
  • 2013
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

플라즈마 메디신: 저온 상압 플라즈마는 어떻게 의학분야에 적용될 수 있는가?

Plasma Medicine: How can Nonthermal Atmospheric Plasma be Applied to Medicine?

  • 박상례 (부산대학교 치의학 전문대학원 구강해부학 교실) ;
  • 홍진우 (부산대학교 한의학 전문대학원) ;
  • 이해준 (부산대학교 전자전기공학부) ;
  • 김규천 (부산대학교 치의학 전문대학원 구강해부학 교실)
  • Park, Sang Rye (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Hong, Jin Woo (Department of Korean Internal Medicine, School of Korean Medicine, Pusan National University) ;
  • Lee, Hae June (Department of Electronics Engineering, Pusan National University) ;
  • Kim, Gyoo Cheon (Department of Oral Anatomy, School of Dentistry, Pusan National University)
  • 투고 : 2013.06.05
  • 심사 : 2013.06.24
  • 발행 : 2013.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

플라즈마는 물질의 제4의 상태로서 이온화된 가스로 불리며 다양한 활성종 및 수산화기, 하전입자, 이온, 자유 전자, 산소등을 발생시키는 특징을 가지고 있다. 20세기 후반에 플라즈마는 산업현장에서 활발히 사용되고 있으며, 최근 저온 상압 플라즈마 장치가 개발되면서 의생명 분야에 적용되기 시작했다. 저온 상압 플라즈마는 인체조직에 열 손상을 입히지 않을 뿐만 아니라, 암 치료, 살균, 치아미백, 지혈, 상처치유 등에서 높은 효율성을 보이고 있다. 이때 발생되는 활성종은 포유세포나 병원균에 영향을 미치는 것으로 알려져 있다. 또한, 다학제간의 연구를 통해 저온 상압 플라즈마의 활용범위는 다양한 영역으로 넓어지고 있으며, 새로운 첨단 의료기술로서의 가치가 높아지고 있다. 저온 상압 플라즈마가 포유세포와 미생물에 적용된 이후, 지난 10여 년간 급속한 발전을 통해 최근 플라즈마 메디신이란 학문영역으로 성장했다. 본 논문은 저온 플라즈마가 적용되고 있는 분야에 대해 소개하고, 플라즈마 메디신에 대한 이해를 돕고자 한다.

As a forth state of material, plasma is ionized gas, which generates characteristically various reactive species. After late of $20^{th}$ century, plasma has been widely used in industry. After nonthermal atmospheric plasma was developed, it has been applied to biomedical fields. Nonthermal atmospheric plasma does not give thermal damages to human tissues, and it shows the high efficiency in cancer treatment, sterilization, tooth bleaching, coagulation, and wound healing. Because the application of plasma to biomedicine has been expanded through interdisciplinary studies, its value of high medical technology is increasing now. Since nonthermal atmospheric plasma was first applied to the mammalian cells and microorganisms, many valuable studies has been performed for about last 10 years, so that now the new research area called 'plasma medicine' has been formed. This article introduces the recent data resulted from plasma medicine and helps to understand the plasma medicine.

키워드

참고문헌

  1. Badylak, S. F. 2002. The extracellular matrix as a scaffold for tissue reconstruction. Cell Dev Biol 133, 77-83.
  2. Berger, S. B., Coelho, A. S., Oliveira, V. A., Cavalli, V. and Giannini, M. 2008. Enamel susceptibility to red wine staining after 35% hydrogen peroxide bleaching. J Appl Oral Sci 16, 201-204. https://doi.org/10.1590/S1678-77572008000300007
  3. Chauncey, H. H., Glass, R. L. and Alman, J. E. 1989. Dental caries. Principal cause of tooth extraction in a sample of US male adults. Caries Res 23, 200-205. https://doi.org/10.1159/000261178
  4. Chen, W., Huang, J., DU, N., Liu, X. D., LV, G. H., WANG, X. Q., Zhang, G. P., Guo, L. H. and Yang, S. Z. 2012. Deactivation of enterococcus faecalis bacteria by an atmospheric cold plasma brush. Chin Phys Lett 29, 075203. https://doi.org/10.1088/0256-307X/29/7/075203
  5. Choi, B. B., Choi, Y. S., Lee, H. J., Lee, J. K., Kim, U. K. and Kim, G. C. 2012. Nonthermal plasma mediated cancer cell death: targeted cancer treatment. J Therm Sci Tech-Jpn 7, 399-404. https://doi.org/10.1299/jtst.7.399
  6. Choi, J. H., Lee, H. W., Lee, J. K., Hong, J. W. and Kim, G. C. 2013. Low-temperature atmospheric plasma increases the expression of anti-aging genes of skin cells without causing cellular damages. Arch Dermatol Res 305, 133-140. https://doi.org/10.1007/s00403-012-1259-8
  7. Davydov, A. I., Kuchukhid, S. T., Shekhter, A. B., Khanin, A. G., Pekshev, A. V. and Pankratov, V. V. 2004. Clinical evaluation of intraoperative application of air-plasma flow enriched by nitrogen monoxide in operations on the uterus and adnexa. Probl Gynecol Obstet Perinatal 3, 12-17.
  8. Ehlbeck, J., Schnabel, U., Polak, M., Winter, J., Woedtke, T., Brandenburg, R., Hagen, T. and Weltmann, K. D. 2011. Low temperature atmospheric pressure plasma sources for microbial decontamination. J Phys D Appl Phys 44, 013002. https://doi.org/10.1088/0022-3727/44/1/013002
  9. Fridman, G., Shereshevsky, A., Jost, M. M., Brooks, A. D., Fridman, A., Gutsol, A., Vasilets, V. and Friedman, G. 2007. Floating electrode dielectric barrier discharge plasma in air promoting apoptotic behavior in melanoma skin cancer cell lines. Plasma Chem Plasma Process 27, 163-176. https://doi.org/10.1007/s11090-007-9048-4
  10. Fridman, G., Friedman, G., Gutsol, A., Shekhter, A. B., Victor, N., Vasilets, V. N. and Fridman, A. 2008. Applied plasma medicine. Review. Plasma Process Polym 5, 503-533. https://doi.org/10.1002/ppap.200700154
  11. Grigorian, A. S., Grudyanov, A. I., Frolova, O. A., Antipova, Z. P., Yerokhin, A. I., Shekhter, A. B. and Pekshev, A. V. 2001. Application of a new biological factor, exogenous nitric oxide, for the surgical treatment of periodontis. Stomatologiia 80, 80-83.
  12. Gregory, F., Marie, P., Manjula, B., Halim, A., Alexander, F., Alexander, G. and Ari, B. 2006. Blood coagulation and living tissue sterilization by floating-electrode dielectric barrier discharge in air. Plasma Chem Plasma Process 26, 425-442. https://doi.org/10.1007/s11090-006-9024-4
  13. Haywood, V. B. 1999. Current status and recommendations for dentist-prescribed, at-home tooth whitening. Contemp Esthet Restor Pract 3, 2-9.
  14. Heinlin, J., Morfill, G., Landthaler, M., Stolz, W., Isbary, G., Zimmermann, J. L., Shimizu, T. and Karrer, S. 2010. Plasma medicine: possible applications in dermatology. J Dtsch Dermatol Ges 8, 968-976.
  15. Jablonowski, L., Koban, I., Berg, M. H., Kindel, E., Duske, K., Schroder, K., Weltmann, K. D. and Kocher, T. 2013. Elimination of E. Faecalis by a new non-thermal atmospheric pressure plasma handheld device for endodontic treatment. A preliminary investigation. Plasma Process Polym. Early View (Online Version of Record published before inclusion in an issue)
  16. Jebur, M. S. 2012. Impacts of virulence factors of Streptococcus mutans isolates on the pathogenesis of acute vaginitis. Int J Nurs Midwifery 4, 16-20.
  17. Kalghatgi, S., Kelly, C. M., Cerchar, E., Torabi, B., Alekseev, O., Fridman, A., Friedman, G. and Azizkhan-Clifford, J. 2011. Effects of non-thermal plasma on mammalian cells. PLoS One 21, e16270.
  18. Kalghatgi, S., Sameer, U., Fridman, G., Cooper, M., Nagaraj, G., Peddinghaus, M., Balasubramanian, M., Vasilets, V. N., Gutsol, A. F., Fridman, A. and Friedman, G. 2007. Mechanism of blood coagulation by nonthermal atmospheric pressure dielectric barrier discharge plasma. IEEE Trans Plasma Sci 35, 1559-1566. https://doi.org/10.1109/TPS.2007.905953
  19. Kang, S. K., Choi, M. Y., Koo, I. G., Kim, P. Y., Kim, Y. S., Kim, G. J., Mohamed, A. A., Collins, G. J. and Lee, J. K. 2011. Reactive hydroxyl radical-driven oral bacterial inactivation by radio frequency atmospheric plasma. Appl Phys Lett 98, 143702. https://doi.org/10.1063/1.3574639
  20. Kim, C. H. New conversing technology; plasma medicine. 2010. Korean J Otorhinolaryngol-Head Neck Surg 53, 593-602. https://doi.org/10.3342/kjorl-hns.2010.53.10.593
  21. Kim, C. H., Kwon, S., Bahn, J. H., Lee, K., Jun, S.I., Rack, P. D. and Baek, S. J. 2010. Effects of atmospheric nonthermal plasma on invasion of colorectal cancer cells. Appl Phys Lett 96, 243701. https://doi.org/10.1063/1.3449575
  22. Kim, D., Gweon, B., Kim, D. B., Choe, W. and Shin, J. H. 2009. A feasibility study for the cancer therapy using cold plasma. 13th Int Conf Biomed Eng 23, 355-357.
  23. Kim, G. C., Kim, G. J., Park, S. R., Jeon, S. M., Seo, H. J., Iza, F. and Lee, J. K. 2009. Air plasma coupled with antibody-conjugated nanoparticles: a new weapon against cancer. J Phys D Appl Phys 42, 032005. https://doi.org/10.1088/0022-3727/42/3/032005
  24. Kim, G. J., Kim, W., Kim, K. T. and Lee, J. K. 2010. DNA damage and mitochondria dysfunction in cell apoptosis induced by nonthermal air plasma. Appl Phys Lett 96, 021502. https://doi.org/10.1063/1.3292206
  25. Koban, I., Matthes, R., Hübner, N. O., Welk, A., Meisel, P., Holtfreter, B., Sietmann, R., Kindel, E., Weltmann, K. D., Kramer, A. and Kocher, T. 2010. Treatment of Candida albicans biofilms with low-temperature plasma induced by dielectric barrier discharge and atmospheric pressure plasma jet. New J Phys 12, 073039. https://doi.org/10.1088/1367-2630/12/7/073039
  26. Kong, M. G., Keidar, M. and Ostrikov, K. 2011. Plasmas meet nanoparticles-where synergies can advance the frontier of medicine. J Phys D Appl Phys 44, 174014-174018. https://doi.org/10.1088/0022-3727/44/17/174014
  27. Laroussi, M. 2009. Low-temperature plasmas for medicine?. IEEE Trans Plasma Sci 37, 714-725. https://doi.org/10.1109/TPS.2009.2017267
  28. Lee, H. J., Shon, C. H., Kim, Y. S., Kim, S., Kim, G. C. and Kong, M. G. 2009. Degradation of adhesion molecules of G361 melanoma cells by a non-thermal atmospheric pressure microplasma. New J Phys 11, 1-13.
  29. Lee, H. W., Kim, G. J., Kim, J. M., Park, J. K., Lee, J. K. and Kim, G. C. 2009. Tooth bleaching with nonthermal atmospheric pressure plasma. J Endod 35, 587-591. https://doi.org/10.1016/j.joen.2009.01.008
  30. Lee, H. W., Nam, S. H., Mohamed, A. A., Kim, G. C. and Lee, J. K. 2010. Atmospheric pressure plasma jet composed of three electrodes: application to tooth bleaching. Plasma Process Polym 7, 274-280. https://doi.org/10.1002/ppap.200900083
  31. Lim, M. Y., Lum, S. O., Poh, R. S., Lee, G. P. and Lim, K. C. 2004. An in vitro comparison of the bleaching efficacy of 35% carbamide peroxide with established intracoronal bleaching agents. Int Endod J 37, 483-488. https://doi.org/10.1111/j.1365-2591.2004.00829.x
  32. Luddin, N. and Ahmed, H. M. 2013. The antibacterial activity of sodium hypochlorite and chlorhexidine against Enterococcus faecalis: A review on agar diffusion and direct contact methods. J Conserv Dent 16, 9-16. https://doi.org/10.4103/0972-0707.105291
  33. Mahasneh, A., Darby, M., Tolle, S. L., Hynes, W., Laroussi, M. and Karakas, E. 2011. Inactivation of Porphyromonas gingivalis by low-temperature atmospheric pressure plasma. Plasma Med 1, 191-204. https://doi.org/10.1615/PlasmaMed.2012002854
  34. Marchal, F., Robert, H., Merbahi, N., Fontagné-Faucher, C., Yousfi, M., Romain, C. E., Eichwald, O., Rondel, C. and Gabriel, B. 2012. Inactivation of Gram-positive biofilms by low-temperature plasma jet at atmospheric pressure. J Phys D Appl Phys 45, 345202. https://doi.org/10.1088/0022-3727/45/34/345202
  35. Morfill, G. E., Kong, M. G. and Zimmermann, J. L. 2009. Focus on plasma medicine. New J Phys 11, 115011. https://doi.org/10.1088/1367-2630/11/11/115011
  36. Morfill, G. E., Shimizu, T., Steffes, B. and Schmidt, H. U. 2009. Nosocomial infections-a new approach towards preventive medicine using plasmas. New J Phys 11, 115019. https://doi.org/10.1088/1367-2630/11/11/115019
  37. Murakami, T., Niemi, K., Gans, T., O'Connell, D. and Graham, W. G. 2013. Chemical kinetics and reactive species in atmospheric pressure helium-oxygen plasmas with humid- air impurities. Plasma Sources Sci Technol 22, 015003. https://doi.org/10.1088/0963-0252/22/1/015003
  38. Nam, S. H., Lee, H. W., Cho, S. H., Lee, J. K., Jeon, Y. C. and Kim, G. C. 2013. High-efficiency tooth bleaching using nonthermal atmospheric pressure plasma with low concentration of hydrogen peroxide. J Appl Oral Sci (In press)
  39. Park, J. K., Nam, S. H., Kwon, H. C, Mohamed, A. A., Lee, J. K. and Kim, G. C. 2011. Feasibility of nonthermal atmospheric pressure plasma for intracoronal bleaching. Int Endod J 44, 170-175. https://doi.org/10.1111/j.1365-2591.2010.01828.x
  40. Park, S. J., Choi, J., Park, G. Y., Lee, S. K., Cho, Y. S., Yun, J. I., Jeon, S. M., Kim, K. T., Lee, J. K. and Sim, J. Y. 2010. Inactivation of S. mutans using an atmospheric plasma driven by a palm-size-integrated microwave power module. IEEE Trans Plasma Sci 8, 1956-1962.
  41. Petersen, P. E., Bourgeois, D., Ogawa, H., Estupinan-Day. S. and Ndiaye, C. 2005. The global burden of oral diseases and risks to oral health. Bull World Health Organ 83, 661-669.
  42. Sensenig, R., Kalghatgi, S., Cerchar, E., Fridman, G., Shereshevsky, A., Torabi, B., Arjunan, K. P., Podolsky, E., Fridman, A., Friedman, G., Azizkhan-Clifford, J. and Brooks, A. D. 2011. Non-thermal plasma induces apoptosis in melanoma cells via production of intracellular reactive oxygen species. Ann Biomed Eng 39, 674-687. https://doi.org/10.1007/s10439-010-0197-x
  43. Shekhter, A. B., Serezhenkov, V. A., Rudenko, T. G., Pekshev, A. V. and Vanin, A. F. 2005. Beneficial effect of gaseous nitric oxide on the healing of skin wounds. Nitric oxide 12, 210-219. https://doi.org/10.1016/j.niox.2005.03.004
  44. Sladek, R. E., Filoche, S. K., Sissons, C. H. and Stoffels, E. 2007. Treatment of Streptococcus mutans biofilms with a nonthermal atmospheric plasma. Lett Appl Microbiol 45, 318-323. https://doi.org/10.1111/j.1472-765X.2007.02194.x
  45. Sottile, J., Hocking, D. C. and Langenbach, K. J. 2000. Fibronectin polymerization stimulates cell growth by RGD-dependent and -independent mechanisms. J Cell Sci 113, 4287-4299.
  46. Stefan, R., Lehmann, A., Hannig, M., Barbara, S. F., Schubert, A., Feldmann, U. and Schindler, A. 2010. Killing of adherent oral microbes by a non-thermal atmospheric plasma jet. J Med Microbiol 59, 206-212. https://doi.org/10.1099/jmm.0.013714-0
  47. Stoffels, E., Kieft I. E., Sladek, R. E. J., van den Bedem, L. J. M., van der Laan, E. P. and teinbuch, M. 2006. Plasma needle for in vivo medical treatment: recent developments and perspectives. Plasma Sources Sci Technol 15, S169-S180 https://doi.org/10.1088/0963-0252/15/4/S03
  48. Sureshkumar, A., Sankar, R., Mandal, M. and Neogi, S. 2010. Effective bacterial inactivation using low temperature radio frequency plasma. Int J Pharm 396, 17-22. https://doi.org/10.1016/j.ijpharm.2010.05.045
  49. Tenuta, L. M., Zamataro, C. B., Del Bel Cury, A. A., Tabchoury, C. P. and Cury, J. A. 2009. Mechanism of fluoride dentifrice effect on enamel demineralization. Caries Res 43, 278-285. https://doi.org/10.1159/000217860
  50. Thiyagarajan, M., Gonzales, X. F. and Anderson, H. 2013. Regulated cellular exposure to non-thermal plasma allows preferentially directed apoptosis in acute monocytic leukemia cells. Stud Health Technol Inform 184, 436-442.
  51. Tiwari P. K., Kang, S. K., Kim G. J., Choi, J., Mohamed A. A. and Lee, J. K. 2009. Modeling of nanoparticle-mediated electric field enhancement inside biological cells exposed to AC electric fields. Jpn J Appl Phys 48, 087001. https://doi.org/10.1143/JJAP.48.087001
  52. Langmuir, I. 1928. Oscillations in ionized gases. Proc Natl Acad Sci USA 14, 627-637. https://doi.org/10.1073/pnas.14.8.627
  53. Tuhvatulin, A. I., Sysolyatina, E. V., Scheblyakov, D.V., Logunov, D.Y., Vasiliev, M. M., Yurova, M. A., Danilova, M. M., Petrov, O. F., Naroditsky, B. S., Morfill, G. E., Grigoriev, A.I., Fortov, V. E., Gintsburg, A. L. and Ermolaeva, S. A. 2012. Non-thermal plasma causes p53-dependent apoptosis in human colon carcinoma cells. Acta Naturae 4, 82-87.
  54. Upadhyaya, C. and Humagain, M. 2009. The pattern of tooth loss due to dental caries and periodontal disease among patients attending dental department (OPD), Dhulikhel Hospital, Kathmandu University Teaching Hospital (KUTH), Nepal. Kathmandu Univ Med J 7, 59-62.
  55. Vandamme, M., Robert, E., Pesnel, S., Barbosa, E., Dozias, S., Sobilo, J., Lerondel, S., Pape, A. L. and Pouvesle, J. M. 2010. Antitumor effect of plasma treatment on U87 Glioma Xenografts: Preliminary results. Plasma Process Polym 7, 264-273. https://doi.org/10.1002/ppap.200900080
  56. Watts, A. and Addy, M. 2001. Tooth discolouration and staining: a review of the literature. Britdent J 190, 309-316.
  57. Williams, J. M., Trope, M., Caplan, D. J. and Shugars, D. C. 2006. Detection and quantitation of E. faecalis by Real-time PCR (qPCR), reverse transcription-PCR (RT-PCR), and cultivation during endodontic treatment. J Endod 32, 715-721. https://doi.org/10.1016/j.joen.2006.02.031
  58. Yang, B., Chen, J., Yu, Q., Li, H., Lin, M., Mustapha, A., Hong, L. and Wang, Y. 2011. Oral bacterial deactivation using a low-temperature atmospheric argon plasma brush. J Dent 39, 48-56. https://doi.org/10.1016/j.jdent.2010.10.002
  59. Yusupov, M., Neyts, E. C., Khalilov, U., Snoeckx, R., van Duin, A. C. T. and Bogaerts, A. 2012. Atomic-scale simulations of reactive oxygen plasma species interacting with bacterial cell walls. New J Phys 14, 093043. https://doi.org/10.1088/1367-2630/14/9/093043
  60. Zhang, X., Li, M., Zhou, R., Feng, K. and Yang, S. 2008. Ablation of liver cancer cells in vitro by a plasma needle. Appl Phys Lett 93, 021502. https://doi.org/10.1063/1.2959735

피인용 문헌

  1. Dentinal Tubules Occluding Effect Using Nonthermal Atmospheric Plasma vol.43, pp.2, 2018, https://doi.org/10.11620/IJOB.2018.43.2.083