생명과학회지 (Journal of Life Science)

  • 제23권6호
  • /
  • Pages.789-795
  • /
  • 2013
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

발효누에분말 투여가 orotic acid 유발 흰쥐에 미치는 지방간 개선 효과

Improvement Effect of Fermented Silkworm (Bombyx mori L.) Powder against Orotic Acid-induced Fatty Liver in Rats

  • 안희영 (동아대학교 대학원 의생명과학과) ;
  • 차재영 (대선주조(주)기술연구소) ;
  • 박규림 (동아대학교 대학원 의생명과학과) ;
  • 김유라 (동아대학교 생명공학과) ;
  • 조영수 (동아대학교 생명공학과)
  • Ahn, Hee-Young (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Cha, Jae-Young (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Park, Kyu-Rim (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Kim, Yu-Ra (Department of Biotechnology, Dong-A University) ;
  • Cho, Young-Su (Department of Biotechnology, Dong-A University)
  • 투고 : 2013.04.23
  • 심사 : 2013.06.21
  • 발행 : 2013.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

단백질 분해 활성, 이화학적 특성 및 생리활성을 검토한 결과 우수한 효능을 나타낸 Bacillus subtilis 및 Aspergillus kawachii 균주를 이용하여 Orotic acid-유발 지방간의 개선 효과를 알아보기 위해 발효된 누에 분말을 식이에 5% 수준으로 첨가하였다. 발효 누에 분말은 Orotic acid 지방간 유발 흰쥐의 간 조직 및 혈청 중성지질 농도를 낮춰주고, 혈청 지질농도를 정상 수준으로 올려주어 지방간 개선에 도움을 주었다. SP 투여군과 AFSP 투여군에 반해, BFSP 투여군에서 지질대사와 관련된 개선 효과를 보였고, 간 조직의 병리학적 관찰결과에서도 BFSP 투여군이 간소엽을 구성하고 있는 간 세포들이 전반적으로 균일하게 배열되어 있어 정상군(N)의 간 소견과 비슷한 양상을 보여 지방간의 개선효과가 있는 것으로 보였다. 이러한 결과를 바탕으로 발효 누에 분말은 향후 지방간 개선효능을 가지는 건강식품 개발 가능성이 높은 소재로 판단되어 진다.

Current study was to investigate the potential effects of silkworm, Bombyx mori L. fermented with Bacillus subtilis (BFSP) and Aspergillus kawachii (AFSP) at the 5% (w/w) levels in Sprague-Dawley rats, which was intoxicated with 1% (w/w) orotic acid (OA) for 10 days. The rat group administered silkworm powder showed improvements in fatty liver condition. Consumption of fermented silkworm powder reduced triglyceride concentrations in the liver tissues and serum and increased the serum lipid concentrations to normal levels, thereby aiding in improving fatty liver conditions. These effects were more pronounced in the BFSP than that in SP or AFSP in orotic acid-induced hepatotoxicity and oxidative stress. Based on these results, fermented silkworms are considered to be a material with significant potential for development into a functional health food that can improve fatty liver conditions.

키워드

참고문헌

  1. Bennett, J. W. 2001. Aspergillus and koji: history, practice and molecular biology. SIM News 51, 65-71.
  2. Bunang, Y., Wang, Y. M., Cha, J. Y., Nagao, K. and Yanagita, T. 2005. Dietary phosphatidylcholine alleviates fatty liver induced by orotic acid. J Korean Soc Food Sci Nutr 21, 867-873.
  3. Cha, J. Y., Heo, J. S. and Cho, Y. S. 2003. Effect of zinc-enriched yeast FF-10 strain on the alcoholic hepatotoxicity in alcohol feeding rats. Food Sci Biotechnol 17, 1207-1213.
  4. Cha, J. Y., Cho, Y. S., Kim, I., Anno, T., Rahman, S. M. and Yanagita, T. 2001. Effect of hesperetin, a citrus flavonoid, on the liver triacylglycerol content and phosphatidate phosphohydrolase activity in orotic acid-fed rats. Plant Foods Hum Nutr 56, 349-358. https://doi.org/10.1023/A:1011884200848
  5. Cha, J. Y., Kim, Y. S., Kang, P. D., Ahn, H. Y., Eom, K. E. and Cho, Y. S. 2010. Biological activity and chemical characteristics of fermented silkworm powder by mold. J Life Sci 20, 237-244. https://doi.org/10.5352/JLS.2010.20.2.237
  6. Cha, J. Y. and Cho, Y. S. 2001. Effects of orotic acid and di-(2-ethylhexylphthalate on microsomal triglyceride transfer protein (MTP) and mRNA levels in liver and intestine of rats. Korean J Food Sci Technol 33, 492-496.
  7. Cha, J. Y., Jun, B. S. and Cho, Y. S. 2004. Prevention of orotic acid-induced fatty liver in rats by capsaicin. Food Sci Biotechnol 13, 597-602.
  8. Cha, J. Y., Maeda, Y., Oogami, K., Yamamoto, K. and Yanagita, T. 1998. Association between hepatic triacylglycerol accumulation induced by administering orotic acid and enhenced phospatidate phosphohydrase activity in rats. Biosci Biotechnol Biochem 62, 508-513. https://doi.org/10.1271/bbb.62.508
  9. Cha, J. Y., Kim, Y. S., Ahn, H. Y., Eom, K. E., Park, B. K. and Cho, Y. S. 2009. Biological activity of fermented silkworm powder. J Life Sci 19, 1468-1477. https://doi.org/10.5352/JLS.2009.19.10.1468
  10. Duncan, D. B. 1957. Multiple range test for correlated and heteroscedastic means. Biometrics 13, 164-176. https://doi.org/10.2307/2527799
  11. Esaki, H., Kawakishi, S., Morimitsu, Y. and Osawa, T. 1999. New potent antioxid ative o-dihydroxylisoflavones in fermented Japanese soybean products. Biosci Biotechnol Biochem 63, 1637-1639. https://doi.org/10.1271/bbb.63.1637
  12. Folch, J., Lees, M. and Sloan-Stanley, G. H. 1957. A simple method for isolation and purification of total lipids from animal tissues. J Biol Chem 226, 497-509.
  13. Hebbachi, A. M., Seelaender, M. C., Baker, B. W. and Gibbons, G. F. 1997. Decreased secretion of very-low-density lipoprotein triglyceride and apolipoprotein B is associated with decreased intercellular triglyceride lipolysis in hepatocytes derived from rats fed orotic acid or n-3 fatty acids. Biochem J 325, 711-719.
  14. Kang, G. D., Lee, K. H., Do, S. G., Kim, C. S., Suh, J. G., Oh, Y. S. and Nham, J. H. 2001. Effect of silk fibroin on the protection of alcoholic hepatotoxicity in the liver of alcohol preference mouse. Int J Indust Entomol 2, 15-18.
  15. Kang, P. D., Kim, J. W., Sohn, B. H., Kim, K. Y., Jung, I. Y., Kim, M. J. and Ryu, K. S. 2000. Accumulating pattern of ${\alpha}$-glycosidase inhibitor in various silkworm varities. Korean J Seric Sci 48, 25-27.
  16. Kim, Y. S., Kim, K. Y., Kang, P. D., Cha, J. Y., Heo, J. S., Park, B. K. and Cho, Y. S. 2008. Effect of silkworm (Bombyx mori L.) excrement powder on the alcoholic hepatotoxicity in rats. J Life Sci 18, 1342-1347. https://doi.org/10.5352/JLS.2008.18.10.1342
  17. Lieber, C. S. 1997. Ethanol metabolism, cirrhosis and alcoholism. Clin Chim Acta 257, 59-84. https://doi.org/10.1016/S0009-8981(96)06434-0
  18. McGrowan, M. W., Artiss, J. D., Strandbergh, D. R. and Zak, B. 1983. A peroxidase-coupled method for the colorimetric determination of serum triglycerides. Clin Chem 29, 538-542.
  19. Park, Y. J., Heo, J. C., An, S. M., Yun, E. Y., Han, S. M., Hwang, J. S., Kang, S. W., Yun, C. Y. and Lee, S. H. 2005. High throughput-compatible screening of antioxidative substances by insect extract library. Korean J Food Preserv 12, 482-488.
  20. Pottenger, L. A. and Getz, G. S. 1971. Serum lipoprotein accumulation in the livers of orotic acid fed rats. J Lipid Res 12, 450.
  21. Ryu, H. Y., Heo, J. C., Hwang, J. S., Kang, S. W., Yun, C. Y., Lee, S. H. and Sohn, H. Y. 2008. Screening of thrombin inhibitor and its DPPH radical scavenging activity from wide insects. J Life Sci 18, 363-368. https://doi.org/10.5352/JLS.2008.18.3.363
  22. Silverman, J. F., Pories, W. J. and Caro, J. F. 1989. Liver pathology in diabetes mellitus and morbid obesity. Clincal, pathological, and biochemical concentrations. Pathol Annu 24, 275-302.
  23. Yoon, J. W., Lee, S. G. and Lee, K. B. 2005. Effects of silkworm extract powder on plasma lipids and glucose in rat. Korean J Food Nutr 18, 140-145.