Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Valproic Acid Regulates α-Synuclein Expression through JNK Pathway in Rat Primary Astrocytes

  • Kim, Jung Nam (Department of Neuroscience, SMART Institute Advanced Biomedical Sciences, School of Medicine, Konkuk University) ;
  • Kim, Min Kyeong (Department of Neuroscience, SMART Institute Advanced Biomedical Sciences, School of Medicine, Konkuk University) ;
  • Cho, Kyu Suk (Department of Neuroscience, SMART Institute Advanced Biomedical Sciences, School of Medicine, Konkuk University) ;
  • Choi, Chang Soon (Department of Neuroscience, SMART Institute Advanced Biomedical Sciences, School of Medicine, Konkuk University) ;
  • Park, Seung Hwa (Department of Anatomy, SMART Institute Advanced Biomedical Sciences, School of Medicine, Konkuk University) ;
  • Yang, Sung-Il (Department of Pharmacology, SMART Institute Advanced Biomedical Sciences, School of Medicine, Konkuk University) ;
  • Joo, So Hyun (Department of Neuroscience, SMART Institute Advanced Biomedical Sciences, School of Medicine, Konkuk University) ;
  • Park, Jin Hee (Department of Neuroscience, SMART Institute Advanced Biomedical Sciences, School of Medicine, Konkuk University) ;
  • Bahn, Geonho (Department of Psychiatry, College of Medicine, Kyung Hee University) ;
  • Shin, Chan Young (Department of Neuroscience, SMART Institute Advanced Biomedical Sciences, School of Medicine, Konkuk University) ;
  • Lee, He-Jin (Department of Anatomy, SMART Institute Advanced Biomedical Sciences, School of Medicine, Konkuk University) ;
  • Han, Seol-Heui (Department of Neuroscience, SMART Institute Advanced Biomedical Sciences, School of Medicine, Konkuk University) ;
  • Kwon, Kyoung Ja (Department of Neuroscience, SMART Institute Advanced Biomedical Sciences, School of Medicine, Konkuk University)
  • Received : 2013.01.17
  • Accepted : 2013.05.02
  • Published : 2013.05.31
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Abstract

Although the role of ${\alpha}$-synuclein aggregation on Parkinson's disease is relatively well known, the physiological role and the regulatory mechanism governing the expression of ${\alpha}$-synuclein are unclear yet. We recently reported that ${\alpha}$-synuclein is expressed and secreted from cultured astrocytes. In this study, we investigated the effect of valproic acid (VPA), which has been suggested to provide neuroprotection by increasing ${\alpha}$-synuclein in neuron, on ${\alpha}$-synuclein expression in rat primary astrocytes. VPA concentration-dependently increased the protein expression level of ${\alpha}$-synuclein in cultured rat primary astrocytes with concomitant increase in mRNA expression level. Likewise, the level of secreted ${\alpha}$-synuclein was also increased by VPA. VPA increased the phosphorylation of Erk1/2 and JNK and pretreatment of a JNK inhibitor SP600125 prevented the VPA-induced increase in ${\alpha}$-synuclein. Whether the increased ${\alpha}$-synuclein in astrocytes is involved in the reported neuroprotective effects of VPA awaits further investigation.

Keywords

References

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