Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Protective Effect of Fisetin (3,7,3',4'-Tetrahydroxyflavone) against γ-Irradiation-Induced Oxidative Stress and Cell Damage

  • Piao, Mei Jing (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Kim, Ki Cheon (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Chae, Sungwook (Aging Research Center, Korea Institute of Oriental Medicine) ;
  • Keum, Young Sam (Department of Biochemistry, College of Pharmacy, Dongguk University) ;
  • Kim, Hye Sun (Cancer Research Institute, Seoul National University College of Medicine) ;
  • Hyun, Jin Won (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University)
  • Received : 2013.02.07
  • Accepted : 2013.03.20
  • Published : 2013.05.31
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Abstract

Ionizing radiation can induce cellular oxidative stress through the generation of reactive oxygen species, resulting in cell damage and cell death. The aim of this study was to determine whether the antioxidant effects of the flavonoid fisetin (3,7,3',4'-tetrahydroxyflavone) included the radioprotection of cells exposed to ${\gamma}$-irradiation. Fisetin reduced the levels of intracellular reactive oxygen species generated by ${\gamma}$-irradiation and thereby protected cells against ${\gamma}$-irradiation-induced membrane lipid peroxidation, DNA damage, and protein carbonylation. In addition, fisetin maintained the viability of irradiated cells by partially inhibiting ${\gamma}$-irradiation-induced apoptosis and restoring mitochondrial membrane potential. These effects suggest that the cellular protective effects of fisetin against ${\gamma}$-irradiation are mainly due to its inhibition of reactive oxygen species generation.

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References

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